Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis

Amy S. Weinmann, Pearlly S. Yan, Matthew J. Oberley, Tim Hui Ming Huang, Peggy J. Farnham

Research output: Contribution to journalArticle

387 Citations (Scopus)

Abstract

Previously, identification of promoters regulated by mammalian transcription factors has relied upon overexpression studies. Here we present the identification of a large set of promoters that are bound by E2F in physiological conditions. Probing a human CpG microarray with chromatin immunoprecipitated using an antibody to E2F4, we have identified 68 unique target loci; 15% are bidirectional promoters and 25% recruit E2F via a mechanism distinct from the defined consensus site. Interestingly, although E2F has been shown previously to regulate genes involved in cell cycle progression, many of the new E2F target genes encode proteins involved in DNA repair or recombination. We suggest that human CpG microarrays, in combination with chromatin immunoprecipitation, will allow rapid identification of target promoters for many mammalian transcription factors.

Original languageEnglish (US)
Pages (from-to)235-244
Number of pages10
JournalGenes and Development
Volume16
Issue number2
DOIs
StatePublished - Jan 15 2002
Externally publishedYes

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CpG Islands
Chromatin Immunoprecipitation
Microarray Analysis
Transcription Factors
DNA Repair
Genetic Recombination
Chromatin
Cell Cycle
Antibodies
Genes
Proteins

Keywords

  • Chromatin immunoprecipitation
  • CpG island microarray
  • E2F
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis. / Weinmann, Amy S.; Yan, Pearlly S.; Oberley, Matthew J.; Huang, Tim Hui Ming; Farnham, Peggy J.

In: Genes and Development, Vol. 16, No. 2, 15.01.2002, p. 235-244.

Research output: Contribution to journalArticle

Weinmann, Amy S. ; Yan, Pearlly S. ; Oberley, Matthew J. ; Huang, Tim Hui Ming ; Farnham, Peggy J. / Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis. In: Genes and Development. 2002 ; Vol. 16, No. 2. pp. 235-244.
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