Isoform-specific binding of the tyrosine phosphatase PTPσ to a ligand in developing muscle

Gustavo Sajnani-Perez, John K. Chilton, A. Radu Aricescu, Fawaz Haj, Andrew W. Stoker

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


PTPσ is a receptor tyrosine phosphatase that is expressed widely in the developing nervous system and that controls the growth and retinotopic mapping of retinal axons. PTPσ is also expressed in motor neurons where its function is unclear. Given that invertebrate relatives of PTPσ can control motor axon guidance, target contact, and synaptogenesis, we have asked if extracellular ligands exist for cPTPσ, the avian PTPσ orthologue, in the neuromuscular system. Of the two major isoforms cPTPσ1 and cPTPσ2, only the shorter cPTPσ1 isoform is expressed in developing spinal motor neurons and their axons. We show that ectodomains of cPTPσ1, but not of cPTPσ2, bind specifically to developing skeletal myotubes. The putative myotube ligand is not related to the previously described binding of cPTPσ to heparan sulfates within the proteoglycans agrin and collagen XVIII, since heparinase treatment of myotubes does not alter cPTPσ1 binding and since most mutations that abolish binding of cPTPσ1 to heparin do not affect myotube binding. The expression of cPTPσ1 in motor axons and its direct binding to target myotubes suggest an isoform-specific role for axonally expressed cPTPσ1 during establishment or maintenance of neuromuscular contacts.

Original languageEnglish (US)
Pages (from-to)37-48
Number of pages12
JournalMolecular and Cellular Neuroscience
Issue number1
StatePublished - Jan 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience


Dive into the research topics of 'Isoform-specific binding of the tyrosine phosphatase PTPσ to a ligand in developing muscle'. Together they form a unique fingerprint.

Cite this