Isoflurane potency in the northern leopard frog Rana pipiens is similar to that in mammalian species and is unaffected by decerebration

Linda S Barter, L. O. Mark, A. C. Smith, J. F. Antognini

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Amphibians are commonly used in biomedical research, including studies of mechanisms of anaesthetic action. There is, however, little published work describing the kinetics of inhaled anaesthetic agents or the potency of isoflurane in amphibians. Ten Northern leopard frogs were exposed to a constant isoflurane concentration of 1.0%, 1.2% or 1.5% atm for 4 h, and their response to a noxious stimulus was tested every 20 min. Each frog was anaesthetized with each concentration in random order and allowed at least 16 h to recover between anaesthetic exposures. Frogs were then pithed and the protocol was repeated. Frogs first displayed immobility during stimulus application at 80 min, and the proportion of animals becoming immobile steadily increased to reach a stable level at 4 h. The 50% effective dose for isoflurane in intact and pithed frogs did not differ, and was 1.15 and 1.25% atm, respectively. The potency of isoflurane in leopard frogs was similar to that reported in mammalian species. Cutaneous uptake of anaesthetic is effective given sufficient time, approximately 4 h in this study. Forebrain structures appear to be unimportant for the immobilizing action of isoflurane in the frog.

Original languageEnglish (US)
Pages (from-to)757-763
Number of pages7
JournalVeterinary Research Communications
Volume31
Issue number6
DOIs
StatePublished - Aug 2007

Keywords

  • Amphibian
  • Anaesthesia
  • Anaesthetic potency
  • Frog
  • Inhalants
  • Isoflurane

ASJC Scopus subject areas

  • veterinary(all)

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