Is there a synergistic effect between mixed bacterial infection in biofilm formation on biliary stents?

Joseph Leung, Lei Liu Yan Lei Liu, T. Desta, E. Libby, J. F. Inciardi, K. Lam

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Background: Biliary sludge which forms as a result of bacterial adherence and biofilm formation in the biliary system is a recognized cause of block-age of plastic stents. Bacteriological cultures of sludge have revealed a mixed infection with gram-positive and gram-negative bacteria. Animal studies have shown that prophylactic ciprofloxacin, which selectively suppress gram-negative bacteria, results in prolonged stent patency despite colonization of the stents by gram-positive bacteria. Methods: We tested a possible synergistic effect between gram-negative and gram-positive bacteria in adherence and biofilm formation on plastic stents. Clinical isolates of Escherichia coli and Enterococcus were cultured in separate chemostats to achieve a steady growth. Adherence of the two bacteria on plastic stent surface were tested separately by perfusing infected bile with the respective bacteria through different modified Robbins devices containing 10F polyethylene stent pieces up to 4 days. In a second experiment, Enterococcus was perfused through stent pieces precolonized with E. coli for 24 hours. The stent pieces were then removed daily and analyzed by bacteriologic culture and scanning electron microscopy for bacterial adherence and biofilm formation. Results: Gram-negative E. coli were more adherent than gram- positive Enterococcus. Precolonization with E. coli facilitates subsequent attachment of Enterococcus. Conclusions: We concluded that there is a synergistic effect between gram-positive and gram-negative bacteria in adherence and biofilm formation.

Original languageEnglish (US)
Pages (from-to)250-257
Number of pages8
JournalGastrointestinal Endoscopy
Volume48
Issue number3
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Is there a synergistic effect between mixed bacterial infection in biofilm formation on biliary stents?'. Together they form a unique fingerprint.

  • Cite this