Is there a Relation between Chlamydia Infection and Primary Biliary Cirrhosis?

Patrick S Leung, Ogyi Park, Shuji Matsumura, Affab A. Ansari, Ross L. Coppel, M. Eric Gershwin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Over the past two decades, a number of studies have failed to provide direct evidence of specific microbial chronic infection in primary biliary cirrhosis (PBC). However, a recent report suggests that there is a specific association of Chlamydia pneumoniae in patients with PBC and that C. pneumoniae or similar antigens might play a role in the pathogenesis of disease. To determine if Chlamydia infection is associated with PBC, we applied a combination of immunological and molecular approaches to investigate (a) the serological reactivity against two common Chlamydia human pathogens, C. pneumoniae and C. trachomatis, by immunoblotting, (b) the presence of Chlamydia in liver samples of patients with PBC and controls by PCR amplification of Chlamydia specific 16S rRNA and (c) the presence of Chlamydia proteins in liver samples of patients with PBC and controls by immunohistochemical staining. By immunoblotting, C. trachomatis and C. pneumoniae specific serological antibodies were found in 52/57 (91.2%) AMA positive PBC, 7/33 (21/2%) of AMA negative PBC, 1/25 (4%) PSC, 0/15 (0%) Sjorgen's syndrome and 0/20 (0%) systemic lupus erythematosus patients and 0/20 (0%) healthy volunteers at 1:200 sera dilution. PBC sera reacted to Chlamydia and E. coli lysates in western blots up to a maximum of 10-4 dilution. However, PCR amplification of the Chlamydia specific 16S rRNA gene was negative in 25/25 PBC livers but positive in 1/4 PSC liver, 3/6 in other liver disease controls and 1/4 normal liver samples. While two commercially available specific monoclonal antibodies stained positive controls (Chlamydia infected HEp-2 cells) they failed to detect Chlamydia antigens in PBC livers. The detection of Chlamydia specific antibodies but not Chlamydia rRNA gene and Chlamydia antigens in PBC suggests that Chlamydia infection is not involved in PBC.

Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalClinical and Developmental Immunology
Volume10
Issue number2-4
DOIs
StatePublished - Jun 2003

Fingerprint

Chlamydia Infections
Biliary Liver Cirrhosis
Chlamydia
Chlamydophila pneumoniae
Liver
rRNA Genes
Immunoblotting
Antigens
Polymerase Chain Reaction
Antibodies
Serum
Systemic Lupus Erythematosus
Liver Diseases
Healthy Volunteers
Western Blotting
Monoclonal Antibodies

Keywords

  • Chlamydia
  • Cross reactivity
  • Infection
  • Primary biliary cirrhosis

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Developmental Biology

Cite this

Is there a Relation between Chlamydia Infection and Primary Biliary Cirrhosis? / Leung, Patrick S; Park, Ogyi; Matsumura, Shuji; Ansari, Affab A.; Coppel, Ross L.; Gershwin, M. Eric.

In: Clinical and Developmental Immunology, Vol. 10, No. 2-4, 06.2003, p. 227-233.

Research output: Contribution to journalArticle

Leung, Patrick S ; Park, Ogyi ; Matsumura, Shuji ; Ansari, Affab A. ; Coppel, Ross L. ; Gershwin, M. Eric. / Is there a Relation between Chlamydia Infection and Primary Biliary Cirrhosis?. In: Clinical and Developmental Immunology. 2003 ; Vol. 10, No. 2-4. pp. 227-233.
@article{2f4ec6bdfe844573b6e7bf1b6ead0b86,
title = "Is there a Relation between Chlamydia Infection and Primary Biliary Cirrhosis?",
abstract = "Over the past two decades, a number of studies have failed to provide direct evidence of specific microbial chronic infection in primary biliary cirrhosis (PBC). However, a recent report suggests that there is a specific association of Chlamydia pneumoniae in patients with PBC and that C. pneumoniae or similar antigens might play a role in the pathogenesis of disease. To determine if Chlamydia infection is associated with PBC, we applied a combination of immunological and molecular approaches to investigate (a) the serological reactivity against two common Chlamydia human pathogens, C. pneumoniae and C. trachomatis, by immunoblotting, (b) the presence of Chlamydia in liver samples of patients with PBC and controls by PCR amplification of Chlamydia specific 16S rRNA and (c) the presence of Chlamydia proteins in liver samples of patients with PBC and controls by immunohistochemical staining. By immunoblotting, C. trachomatis and C. pneumoniae specific serological antibodies were found in 52/57 (91.2{\%}) AMA positive PBC, 7/33 (21/2{\%}) of AMA negative PBC, 1/25 (4{\%}) PSC, 0/15 (0{\%}) Sjorgen's syndrome and 0/20 (0{\%}) systemic lupus erythematosus patients and 0/20 (0{\%}) healthy volunteers at 1:200 sera dilution. PBC sera reacted to Chlamydia and E. coli lysates in western blots up to a maximum of 10-4 dilution. However, PCR amplification of the Chlamydia specific 16S rRNA gene was negative in 25/25 PBC livers but positive in 1/4 PSC liver, 3/6 in other liver disease controls and 1/4 normal liver samples. While two commercially available specific monoclonal antibodies stained positive controls (Chlamydia infected HEp-2 cells) they failed to detect Chlamydia antigens in PBC livers. The detection of Chlamydia specific antibodies but not Chlamydia rRNA gene and Chlamydia antigens in PBC suggests that Chlamydia infection is not involved in PBC.",
keywords = "Chlamydia, Cross reactivity, Infection, Primary biliary cirrhosis",
author = "Leung, {Patrick S} and Ogyi Park and Shuji Matsumura and Ansari, {Affab A.} and Coppel, {Ross L.} and Gershwin, {M. Eric}",
year = "2003",
month = "6",
doi = "10.1080/10446670310001642429",
language = "English (US)",
volume = "10",
pages = "227--233",
journal = "Journal of Immunology Research",
issn = "2314-8861",
publisher = "Hindawi Publishing Corporation",
number = "2-4",

}

TY - JOUR

T1 - Is there a Relation between Chlamydia Infection and Primary Biliary Cirrhosis?

AU - Leung, Patrick S

AU - Park, Ogyi

AU - Matsumura, Shuji

AU - Ansari, Affab A.

AU - Coppel, Ross L.

AU - Gershwin, M. Eric

PY - 2003/6

Y1 - 2003/6

N2 - Over the past two decades, a number of studies have failed to provide direct evidence of specific microbial chronic infection in primary biliary cirrhosis (PBC). However, a recent report suggests that there is a specific association of Chlamydia pneumoniae in patients with PBC and that C. pneumoniae or similar antigens might play a role in the pathogenesis of disease. To determine if Chlamydia infection is associated with PBC, we applied a combination of immunological and molecular approaches to investigate (a) the serological reactivity against two common Chlamydia human pathogens, C. pneumoniae and C. trachomatis, by immunoblotting, (b) the presence of Chlamydia in liver samples of patients with PBC and controls by PCR amplification of Chlamydia specific 16S rRNA and (c) the presence of Chlamydia proteins in liver samples of patients with PBC and controls by immunohistochemical staining. By immunoblotting, C. trachomatis and C. pneumoniae specific serological antibodies were found in 52/57 (91.2%) AMA positive PBC, 7/33 (21/2%) of AMA negative PBC, 1/25 (4%) PSC, 0/15 (0%) Sjorgen's syndrome and 0/20 (0%) systemic lupus erythematosus patients and 0/20 (0%) healthy volunteers at 1:200 sera dilution. PBC sera reacted to Chlamydia and E. coli lysates in western blots up to a maximum of 10-4 dilution. However, PCR amplification of the Chlamydia specific 16S rRNA gene was negative in 25/25 PBC livers but positive in 1/4 PSC liver, 3/6 in other liver disease controls and 1/4 normal liver samples. While two commercially available specific monoclonal antibodies stained positive controls (Chlamydia infected HEp-2 cells) they failed to detect Chlamydia antigens in PBC livers. The detection of Chlamydia specific antibodies but not Chlamydia rRNA gene and Chlamydia antigens in PBC suggests that Chlamydia infection is not involved in PBC.

AB - Over the past two decades, a number of studies have failed to provide direct evidence of specific microbial chronic infection in primary biliary cirrhosis (PBC). However, a recent report suggests that there is a specific association of Chlamydia pneumoniae in patients with PBC and that C. pneumoniae or similar antigens might play a role in the pathogenesis of disease. To determine if Chlamydia infection is associated with PBC, we applied a combination of immunological and molecular approaches to investigate (a) the serological reactivity against two common Chlamydia human pathogens, C. pneumoniae and C. trachomatis, by immunoblotting, (b) the presence of Chlamydia in liver samples of patients with PBC and controls by PCR amplification of Chlamydia specific 16S rRNA and (c) the presence of Chlamydia proteins in liver samples of patients with PBC and controls by immunohistochemical staining. By immunoblotting, C. trachomatis and C. pneumoniae specific serological antibodies were found in 52/57 (91.2%) AMA positive PBC, 7/33 (21/2%) of AMA negative PBC, 1/25 (4%) PSC, 0/15 (0%) Sjorgen's syndrome and 0/20 (0%) systemic lupus erythematosus patients and 0/20 (0%) healthy volunteers at 1:200 sera dilution. PBC sera reacted to Chlamydia and E. coli lysates in western blots up to a maximum of 10-4 dilution. However, PCR amplification of the Chlamydia specific 16S rRNA gene was negative in 25/25 PBC livers but positive in 1/4 PSC liver, 3/6 in other liver disease controls and 1/4 normal liver samples. While two commercially available specific monoclonal antibodies stained positive controls (Chlamydia infected HEp-2 cells) they failed to detect Chlamydia antigens in PBC livers. The detection of Chlamydia specific antibodies but not Chlamydia rRNA gene and Chlamydia antigens in PBC suggests that Chlamydia infection is not involved in PBC.

KW - Chlamydia

KW - Cross reactivity

KW - Infection

KW - Primary biliary cirrhosis

UR - http://www.scopus.com/inward/record.url?scp=0346502827&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346502827&partnerID=8YFLogxK

U2 - 10.1080/10446670310001642429

DO - 10.1080/10446670310001642429

M3 - Article

C2 - 14768955

AN - SCOPUS:0346502827

VL - 10

SP - 227

EP - 233

JO - Journal of Immunology Research

JF - Journal of Immunology Research

SN - 2314-8861

IS - 2-4

ER -