Is Ffh required for export of secretory proteins?

Jinoh Kim, Sharyn Rusch, Joen Luirink, Debra A. Kendall

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

In Escherichia coli, protein export from the cytoplasm may occur via the signal recognition particle (SRP)-dependent pathway or the Sec-dependent pathway. Membrane proteins utilize the SRP-dependent route, whereas many secretory proteins use the cytoplasmic Sec machinery. To examine the possibility that signal peptide hydrophobicity governs which targeting route is utilized, we used a series of PhoA signal sequence mutants which vary only by incremental hydrophobicity changes. We show that depletion of SRP, but not trigger factor, affects all the mutants examined. These results suggest secretory proteins with a variety of signal sequences, as well as membrane proteins, require SRP for export.

Original languageEnglish (US)
Pages (from-to)245-248
Number of pages4
JournalFEBS Letters
Volume505
Issue number2
DOIs
StatePublished - Sep 14 2001
Externally publishedYes

Keywords

  • Escherichia coli
  • phoA
  • Protein export
  • Secretory protein
  • Signal recognition particle
  • Trigger factor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Kim, J., Rusch, S., Luirink, J., & Kendall, D. A. (2001). Is Ffh required for export of secretory proteins? FEBS Letters, 505(2), 245-248. https://doi.org/10.1016/S0014-5793(01)02784-3