Irreversible electroporation ablation of the liver can be detected with ultrasound B-mode and elastography

Joyce T. Au, T. Peter Kingham, Kyonghwa Jun, Dana Haddad, Sepideh Gholami, Kelly Mojica, Sebastien Monette, Paula Ezell, Yuman Fong

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Irreversible electroporation (IRE) is a novel ablation technique that induces permanent membrane permeability and cell death. We are interested in ultrasound B-mode and elastography to monitor IRE ablation in the liver. Methods: Yorkshire pigs underwent IRE ablation of the liver and were imaged with ultrasound B-mode and elastography. Histologic evaluation of cell death by triphenyltetrazolium chloride and hematoxylin and eosin staining was performed. Results: Elastography showed that liver ablated by IRE exhibited increased tissue stiffness with a peak strain ratio of 2.22. The IRE lesion had a discrete border without bubble artifact, and the lesion size significantly correlated with area of cell death on histology. IRE ablation was unaffected by presence of large blood vessels or bile ducts. Conclusion: IRE ablation led to increased tissue stiffness that was detectable by elastography and indicative of cell death. Elastography may complement B-mode ultrasonography to monitor IRE ablation of the liver.

Original languageEnglish (US)
Pages (from-to)787-793
Number of pages7
JournalSurgery (United States)
Volume153
Issue number6
DOIs
StatePublished - Jun 1 2013
Externally publishedYes

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Elasticity Imaging Techniques
Electroporation
Liver
Cell Death
Ablation Techniques
Hematoxylin
Eosine Yellowish-(YS)
Bile Ducts
Artifacts
Blood Vessels
Permeability
Ultrasonography
Histology
Swine
Staining and Labeling
Membranes

ASJC Scopus subject areas

  • Surgery

Cite this

Irreversible electroporation ablation of the liver can be detected with ultrasound B-mode and elastography. / Au, Joyce T.; Kingham, T. Peter; Jun, Kyonghwa; Haddad, Dana; Gholami, Sepideh; Mojica, Kelly; Monette, Sebastien; Ezell, Paula; Fong, Yuman.

In: Surgery (United States), Vol. 153, No. 6, 01.06.2013, p. 787-793.

Research output: Contribution to journalArticle

Au, JT, Kingham, TP, Jun, K, Haddad, D, Gholami, S, Mojica, K, Monette, S, Ezell, P & Fong, Y 2013, 'Irreversible electroporation ablation of the liver can be detected with ultrasound B-mode and elastography', Surgery (United States), vol. 153, no. 6, pp. 787-793. https://doi.org/10.1016/j.surg.2012.11.022
Au, Joyce T. ; Kingham, T. Peter ; Jun, Kyonghwa ; Haddad, Dana ; Gholami, Sepideh ; Mojica, Kelly ; Monette, Sebastien ; Ezell, Paula ; Fong, Yuman. / Irreversible electroporation ablation of the liver can be detected with ultrasound B-mode and elastography. In: Surgery (United States). 2013 ; Vol. 153, No. 6. pp. 787-793.
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AU - Au, Joyce T.

AU - Kingham, T. Peter

AU - Jun, Kyonghwa

AU - Haddad, Dana

AU - Gholami, Sepideh

AU - Mojica, Kelly

AU - Monette, Sebastien

AU - Ezell, Paula

AU - Fong, Yuman

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N2 - Background: Irreversible electroporation (IRE) is a novel ablation technique that induces permanent membrane permeability and cell death. We are interested in ultrasound B-mode and elastography to monitor IRE ablation in the liver. Methods: Yorkshire pigs underwent IRE ablation of the liver and were imaged with ultrasound B-mode and elastography. Histologic evaluation of cell death by triphenyltetrazolium chloride and hematoxylin and eosin staining was performed. Results: Elastography showed that liver ablated by IRE exhibited increased tissue stiffness with a peak strain ratio of 2.22. The IRE lesion had a discrete border without bubble artifact, and the lesion size significantly correlated with area of cell death on histology. IRE ablation was unaffected by presence of large blood vessels or bile ducts. Conclusion: IRE ablation led to increased tissue stiffness that was detectable by elastography and indicative of cell death. Elastography may complement B-mode ultrasonography to monitor IRE ablation of the liver.

AB - Background: Irreversible electroporation (IRE) is a novel ablation technique that induces permanent membrane permeability and cell death. We are interested in ultrasound B-mode and elastography to monitor IRE ablation in the liver. Methods: Yorkshire pigs underwent IRE ablation of the liver and were imaged with ultrasound B-mode and elastography. Histologic evaluation of cell death by triphenyltetrazolium chloride and hematoxylin and eosin staining was performed. Results: Elastography showed that liver ablated by IRE exhibited increased tissue stiffness with a peak strain ratio of 2.22. The IRE lesion had a discrete border without bubble artifact, and the lesion size significantly correlated with area of cell death on histology. IRE ablation was unaffected by presence of large blood vessels or bile ducts. Conclusion: IRE ablation led to increased tissue stiffness that was detectable by elastography and indicative of cell death. Elastography may complement B-mode ultrasonography to monitor IRE ablation of the liver.

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