Iron-dependent modifications of the flower transcriptome, proteome, metabolome, and hormonal content in an <

Damien Sudre, Elain Gutierrez-Carbonell, Giuseppe Lattanzio, Rubén Rellán-Álvarez, Frédéric Gaymard, Gert Wohlgemuth, Oliver Fiehn, Ana Álvarez-Fernández, Angel M. Zamarreño, Eva Bacaicoa, Daniela Duy, Jose María García-Mina, Javier Abadía, Katrin Philippar, Ana Flor López-Millán, Jean François Briat

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Iron homeostasis is an important process for flower development and plant fertility. The role of plastids in these processes has been shown to be essential. To document the relationships between plastid iron homeostasis and flower biology further, a global study (transcriptome, proteome, metabolome, and hormone analysis) was performed of Arabidopsis flowers from wild-type and triple atfer1-3-4 ferritin mutant plants grown under iron-sufficient or excess conditions. Some major modifications in specific functional categories were consistently observed at these three omic levels, although no significant overlaps of specific transcripts and proteins were detected. These modifications concerned redox reactions and oxidative stress, as well as amino acid and protein catabolism, this latter point being exemplified by an almost 10-fold increase in urea concentration of atfer1-3-4 flowers from plants grown under iron excess conditions. The mutant background caused alterations in Fehaem redox proteins located in membranes and in hormone-responsive proteins. Specific effects of excess Fe in the mutant included further changes in these categories, supporting the idea that the mutant is facing a more intense Fe/redox stress than the wild type. The mutation and/or excess Fe had a strong impact at the membrane level, as denoted by the changes in the transporter and lipid metabolism categories. In spite of the large number of genes and proteins responsive to hormones found to be regulated in this study, changes in the hormonal balance were restricted to cytokinins, especially in the mutant plants grown under Fe excess conditions.

Original languageEnglish (US)
Pages (from-to)2665-2688
Number of pages24
JournalJournal of Experimental Botany
Volume64
Issue number10
DOIs
StatePublished - Jul 2013

    Fingerprint

Keywords

  • Ferritin
  • flowers
  • functional genomics
  • iron

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Sudre, D., Gutierrez-Carbonell, E., Lattanzio, G., Rellán-Álvarez, R., Gaymard, F., Wohlgemuth, G., Fiehn, O., Álvarez-Fernández, A., Zamarreño, A. M., Bacaicoa, E., Duy, D., García-Mina, J. M., Abadía, J., Philippar, K., López-Millán, A. F., & Briat, J. F. (2013). Iron-dependent modifications of the flower transcriptome, proteome, metabolome, and hormonal content in an < Journal of Experimental Botany, 64(10), 2665-2688. https://doi.org/10.1093/jxb/ert112