Iridovirus infections among Missouri River sturgeon

Initial characterization, transmission, and evidence for establishment of a carrier state

Tomofumi Kurobe, E. MacConnell, C. Hudson, T. S. McDowell, F. O. Mardones, Ronald Hedrick

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Iridovirus infections of the integument were associated with disease and mortality among hatchery-reared populations of juvenile pallid sturgeon Scaphirhynchus albus and shovelnose sturgeon S. platorynchus from the Missouri River. Virus-infected cells in the integument of fins and body were greatly enlarged, possessed pleomorphic and eccentric nuclei, and exhibited an amphophilic to eosinophilic staining of the cytoplasm in hematoxylin-and-eosin-stained sections. Virus particles found in the host cell cytoplasm were composed of an outer hexagonal capsid measuring 254 nm in diameter and surrounding a dense nucleoid. Despite numerous attempts, the virus could not be propagated on routine cell lines used in fish viral diagnostics or from established cell lines from white sturgeon Acipenser transmontanus, pallid sturgeon, or shovelnose sturgeon. Bath exposures of healthy juvenile pallid sturgeon to a crude extract or a 0.45-μm-filtered extract from the fins of infected fish resulted in transmission of the virus and mortality. At water temperatures of 15°C, the first deaths occurred at approximately 1month;mortality peaked between 50 and 60 d postexposure, after which surviving fish recovered. Presence of the virus was confirmed among dead and moribund pallid sturgeon by both histology and detection of viral DNA by polymerase chain reaction methods. Feeding of infected tissues and cohabitation with virus-infected shovelnose sturgeon also resulted in successful virus transmission to juvenile pallid sturgeon. Virus infections among experimentally exposed pallid sturgeon that recovered from clinical episodes persisted for at least 8.5 months, and these apparently healthy fish transmitted the virus and disease to juvenile pallid sturgeon by cohabitation. The newly describedMissouri River sturgeon iridovirus (MRSIV) as found in pallid sturgeon and shovelnose sturgeon shares many properties with a group of iridoviruses associated with serious skin and gill infections in several species of sturgeon.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalJournal of Aquatic Animal Health
Volume23
Issue number1
DOIs
StatePublished - Apr 13 2011

Fingerprint

Iridovirus
Missouri River
carrier state
sturgeon
virus
river
infection
viruses
cohabitation
Acipenser transmontanus
cytoplasm
fish
virus transmission
mortality
integument
fins
Iridoviridae
cell lines
virus particle
Scaphirhynchus albus

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Iridovirus infections among Missouri River sturgeon : Initial characterization, transmission, and evidence for establishment of a carrier state. / Kurobe, Tomofumi; MacConnell, E.; Hudson, C.; McDowell, T. S.; Mardones, F. O.; Hedrick, Ronald.

In: Journal of Aquatic Animal Health, Vol. 23, No. 1, 13.04.2011, p. 9-18.

Research output: Contribution to journalArticle

@article{d6ffe500d95a43578085d3612a992d73,
title = "Iridovirus infections among Missouri River sturgeon: Initial characterization, transmission, and evidence for establishment of a carrier state",
abstract = "Iridovirus infections of the integument were associated with disease and mortality among hatchery-reared populations of juvenile pallid sturgeon Scaphirhynchus albus and shovelnose sturgeon S. platorynchus from the Missouri River. Virus-infected cells in the integument of fins and body were greatly enlarged, possessed pleomorphic and eccentric nuclei, and exhibited an amphophilic to eosinophilic staining of the cytoplasm in hematoxylin-and-eosin-stained sections. Virus particles found in the host cell cytoplasm were composed of an outer hexagonal capsid measuring 254 nm in diameter and surrounding a dense nucleoid. Despite numerous attempts, the virus could not be propagated on routine cell lines used in fish viral diagnostics or from established cell lines from white sturgeon Acipenser transmontanus, pallid sturgeon, or shovelnose sturgeon. Bath exposures of healthy juvenile pallid sturgeon to a crude extract or a 0.45-μm-filtered extract from the fins of infected fish resulted in transmission of the virus and mortality. At water temperatures of 15°C, the first deaths occurred at approximately 1month;mortality peaked between 50 and 60 d postexposure, after which surviving fish recovered. Presence of the virus was confirmed among dead and moribund pallid sturgeon by both histology and detection of viral DNA by polymerase chain reaction methods. Feeding of infected tissues and cohabitation with virus-infected shovelnose sturgeon also resulted in successful virus transmission to juvenile pallid sturgeon. Virus infections among experimentally exposed pallid sturgeon that recovered from clinical episodes persisted for at least 8.5 months, and these apparently healthy fish transmitted the virus and disease to juvenile pallid sturgeon by cohabitation. The newly describedMissouri River sturgeon iridovirus (MRSIV) as found in pallid sturgeon and shovelnose sturgeon shares many properties with a group of iridoviruses associated with serious skin and gill infections in several species of sturgeon.",
author = "Tomofumi Kurobe and E. MacConnell and C. Hudson and McDowell, {T. S.} and Mardones, {F. O.} and Ronald Hedrick",
year = "2011",
month = "4",
day = "13",
doi = "10.1080/08997659.2011.545697",
language = "English (US)",
volume = "23",
pages = "9--18",
journal = "Journal of Aquatic Animal Health",
issn = "0899-7659",
publisher = "American Fisheries Society",
number = "1",

}

TY - JOUR

T1 - Iridovirus infections among Missouri River sturgeon

T2 - Initial characterization, transmission, and evidence for establishment of a carrier state

AU - Kurobe, Tomofumi

AU - MacConnell, E.

AU - Hudson, C.

AU - McDowell, T. S.

AU - Mardones, F. O.

AU - Hedrick, Ronald

PY - 2011/4/13

Y1 - 2011/4/13

N2 - Iridovirus infections of the integument were associated with disease and mortality among hatchery-reared populations of juvenile pallid sturgeon Scaphirhynchus albus and shovelnose sturgeon S. platorynchus from the Missouri River. Virus-infected cells in the integument of fins and body were greatly enlarged, possessed pleomorphic and eccentric nuclei, and exhibited an amphophilic to eosinophilic staining of the cytoplasm in hematoxylin-and-eosin-stained sections. Virus particles found in the host cell cytoplasm were composed of an outer hexagonal capsid measuring 254 nm in diameter and surrounding a dense nucleoid. Despite numerous attempts, the virus could not be propagated on routine cell lines used in fish viral diagnostics or from established cell lines from white sturgeon Acipenser transmontanus, pallid sturgeon, or shovelnose sturgeon. Bath exposures of healthy juvenile pallid sturgeon to a crude extract or a 0.45-μm-filtered extract from the fins of infected fish resulted in transmission of the virus and mortality. At water temperatures of 15°C, the first deaths occurred at approximately 1month;mortality peaked between 50 and 60 d postexposure, after which surviving fish recovered. Presence of the virus was confirmed among dead and moribund pallid sturgeon by both histology and detection of viral DNA by polymerase chain reaction methods. Feeding of infected tissues and cohabitation with virus-infected shovelnose sturgeon also resulted in successful virus transmission to juvenile pallid sturgeon. Virus infections among experimentally exposed pallid sturgeon that recovered from clinical episodes persisted for at least 8.5 months, and these apparently healthy fish transmitted the virus and disease to juvenile pallid sturgeon by cohabitation. The newly describedMissouri River sturgeon iridovirus (MRSIV) as found in pallid sturgeon and shovelnose sturgeon shares many properties with a group of iridoviruses associated with serious skin and gill infections in several species of sturgeon.

AB - Iridovirus infections of the integument were associated with disease and mortality among hatchery-reared populations of juvenile pallid sturgeon Scaphirhynchus albus and shovelnose sturgeon S. platorynchus from the Missouri River. Virus-infected cells in the integument of fins and body were greatly enlarged, possessed pleomorphic and eccentric nuclei, and exhibited an amphophilic to eosinophilic staining of the cytoplasm in hematoxylin-and-eosin-stained sections. Virus particles found in the host cell cytoplasm were composed of an outer hexagonal capsid measuring 254 nm in diameter and surrounding a dense nucleoid. Despite numerous attempts, the virus could not be propagated on routine cell lines used in fish viral diagnostics or from established cell lines from white sturgeon Acipenser transmontanus, pallid sturgeon, or shovelnose sturgeon. Bath exposures of healthy juvenile pallid sturgeon to a crude extract or a 0.45-μm-filtered extract from the fins of infected fish resulted in transmission of the virus and mortality. At water temperatures of 15°C, the first deaths occurred at approximately 1month;mortality peaked between 50 and 60 d postexposure, after which surviving fish recovered. Presence of the virus was confirmed among dead and moribund pallid sturgeon by both histology and detection of viral DNA by polymerase chain reaction methods. Feeding of infected tissues and cohabitation with virus-infected shovelnose sturgeon also resulted in successful virus transmission to juvenile pallid sturgeon. Virus infections among experimentally exposed pallid sturgeon that recovered from clinical episodes persisted for at least 8.5 months, and these apparently healthy fish transmitted the virus and disease to juvenile pallid sturgeon by cohabitation. The newly describedMissouri River sturgeon iridovirus (MRSIV) as found in pallid sturgeon and shovelnose sturgeon shares many properties with a group of iridoviruses associated with serious skin and gill infections in several species of sturgeon.

UR - http://www.scopus.com/inward/record.url?scp=79953783617&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953783617&partnerID=8YFLogxK

U2 - 10.1080/08997659.2011.545697

DO - 10.1080/08997659.2011.545697

M3 - Article

VL - 23

SP - 9

EP - 18

JO - Journal of Aquatic Animal Health

JF - Journal of Aquatic Animal Health

SN - 0899-7659

IS - 1

ER -