Abstract
A review with over 100 references describes the recent applications of ion-molecule reactions to the study of gas-phase protonated peptides and proteins. The topic is focused specifically on the proton transfer and hydrogen-deuterium exchange reactions of amino acids, peptides, and proteins. A brief background is given of the various methods used for assigning proton affinities and gas-phase basicities. The methods used for measuring the kinetics of deuterium incorporation of charged ion in the presence of a background pressure of deuterating reagents are also described. Ion-molecule reactions are used to determine, among other things, the gas-phase basicities and proton affinities of amino acids, peptides, and proteins, the sites of protonation, intra- and intermolecular interactions, and conformational differences and changes in gas-phase ionic species. Singly charged and multiply charged ions are both covered.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 53-71 |
| Number of pages | 19 |
| Journal | Mass Spectrometry Reviews |
| Volume | 16 |
| Issue number | 2 |
| State | Published - Mar 1997 |
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ASJC Scopus subject areas
- Spectroscopy
Cite this
Ion-molecule reactions as probes of gas-phase structures of peptides and proteins. / Green, M. Kirk; Lebrilla, Carlito B.
In: Mass Spectrometry Reviews, Vol. 16, No. 2, 03.1997, p. 53-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ion-molecule reactions as probes of gas-phase structures of peptides and proteins
AU - Green, M. Kirk
AU - Lebrilla, Carlito B
PY - 1997/3
Y1 - 1997/3
N2 - A review with over 100 references describes the recent applications of ion-molecule reactions to the study of gas-phase protonated peptides and proteins. The topic is focused specifically on the proton transfer and hydrogen-deuterium exchange reactions of amino acids, peptides, and proteins. A brief background is given of the various methods used for assigning proton affinities and gas-phase basicities. The methods used for measuring the kinetics of deuterium incorporation of charged ion in the presence of a background pressure of deuterating reagents are also described. Ion-molecule reactions are used to determine, among other things, the gas-phase basicities and proton affinities of amino acids, peptides, and proteins, the sites of protonation, intra- and intermolecular interactions, and conformational differences and changes in gas-phase ionic species. Singly charged and multiply charged ions are both covered.
AB - A review with over 100 references describes the recent applications of ion-molecule reactions to the study of gas-phase protonated peptides and proteins. The topic is focused specifically on the proton transfer and hydrogen-deuterium exchange reactions of amino acids, peptides, and proteins. A brief background is given of the various methods used for assigning proton affinities and gas-phase basicities. The methods used for measuring the kinetics of deuterium incorporation of charged ion in the presence of a background pressure of deuterating reagents are also described. Ion-molecule reactions are used to determine, among other things, the gas-phase basicities and proton affinities of amino acids, peptides, and proteins, the sites of protonation, intra- and intermolecular interactions, and conformational differences and changes in gas-phase ionic species. Singly charged and multiply charged ions are both covered.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031082985&partnerID=8YFLogxK
M3 - Article
VL - 16
SP - 53
EP - 71
JO - Mass Spectrometry Reviews
JF - Mass Spectrometry Reviews
SN - 0277-7037
IS - 2
ER -