Ion-molecule reactions as probes of gas-phase structures of peptides and proteins

M. Kirk Green, Carlito B Lebrilla

Research output: Contribution to journalArticlepeer-review

221 Scopus citations


A review with over 100 references describes the recent applications of ion-molecule reactions to the study of gas-phase protonated peptides and proteins. The topic is focused specifically on the proton transfer and hydrogen-deuterium exchange reactions of amino acids, peptides, and proteins. A brief background is given of the various methods used for assigning proton affinities and gas-phase basicities. The methods used for measuring the kinetics of deuterium incorporation of charged ion in the presence of a background pressure of deuterating reagents are also described. Ion-molecule reactions are used to determine, among other things, the gas-phase basicities and proton affinities of amino acids, peptides, and proteins, the sites of protonation, intra- and intermolecular interactions, and conformational differences and changes in gas-phase ionic species. Singly charged and multiply charged ions are both covered.

Original languageEnglish (US)
Pages (from-to)53-71
Number of pages19
JournalMass Spectrometry Reviews
Issue number2
StatePublished - Mar 1997

ASJC Scopus subject areas

  • Spectroscopy


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