Ion-molecule reactions as probes of gas-phase structures of peptides and proteins

M. Kirk Green, Carlito B Lebrilla

Research output: Contribution to journalArticle

219 Citations (Scopus)

Abstract

A review with over 100 references describes the recent applications of ion-molecule reactions to the study of gas-phase protonated peptides and proteins. The topic is focused specifically on the proton transfer and hydrogen-deuterium exchange reactions of amino acids, peptides, and proteins. A brief background is given of the various methods used for assigning proton affinities and gas-phase basicities. The methods used for measuring the kinetics of deuterium incorporation of charged ion in the presence of a background pressure of deuterating reagents are also described. Ion-molecule reactions are used to determine, among other things, the gas-phase basicities and proton affinities of amino acids, peptides, and proteins, the sites of protonation, intra- and intermolecular interactions, and conformational differences and changes in gas-phase ionic species. Singly charged and multiply charged ions are both covered.

Original languageEnglish (US)
Pages (from-to)53-71
Number of pages19
JournalMass Spectrometry Reviews
Volume16
Issue number2
StatePublished - Mar 1997

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Phase structure
peptides
Amino Acids, Peptides, and Proteins
Gases
Ions
vapor phases
proteins
Protons
Peptides
Molecules
probes
Deuterium
Alkalinity
amino acids
protons
affinity
molecules
deuterium
ions
Proteins

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Ion-molecule reactions as probes of gas-phase structures of peptides and proteins. / Green, M. Kirk; Lebrilla, Carlito B.

In: Mass Spectrometry Reviews, Vol. 16, No. 2, 03.1997, p. 53-71.

Research output: Contribution to journalArticle

Green, M. Kirk ; Lebrilla, Carlito B. / Ion-molecule reactions as probes of gas-phase structures of peptides and proteins. In: Mass Spectrometry Reviews. 1997 ; Vol. 16, No. 2. pp. 53-71.
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