Prostaglandin E2(PGF2)is known to cown regulate specific macrophage capacities and functions. PGE2is a potent figame for several recently identified prostaglandin receptor subtypes designared J;P. These receptor subtype are found in several tissues, have been clened, characterized pharmacologically and structurally. and are linked to various distinct signal transduction pathways. To date it is not known which receptor subtype (s) macrophages may possess. In this study. we provide eveidence that thioglycolla elicied n peritoneal macrophages possess the EP2 receptor subtype during PGF2down regulation of tumor necrosis factor alpha (TNFα) production. The specific EP2 agn deoxy PGE and 14-deoxy 16,16 dimethyl PGE2were able to down regulate hpopolysaccharide (LPS)-induced macrophage TNF αproduction as effectovely as PGF2 The EP3/EP1 agonist sulprostone and the EP1/EP3 agonist 17 phenol trinor PGE2The EP3/EP1 agonist sulprostone and the EP1/EP3 agonist 17 phenol trinor PGE2were ineffective at down regulating TNF production Moreover. The EP1 receptor antagonist SC-9220 could not block the ability of PGE2to down regulaze TNF α production These data suggest that the EP receptor subtype involved in down regulatioon of TNFα prodaction is EP2. (Funded by NIH grant CA7050).
|Original language||English (US)|
|State||Published - 1997|
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology