Involvement of lymphocyte function-associated antigen-1 (LFA-1) but not ICAM-1 in a radioactive leukocyte cell-mediated immunity (LA-CMI) assay.

B. Fekete, James Hildreth, J. F. Holland, J. G. Bekesi

Research output: Contribution to journalArticle

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Abstract

A number of monoclonal antibodies (Mab) specific to human leukocyte adherence molecules were investigated for their possible function in LA-CMI assay. These data indicate that LFA-1 appears to be directly involved in LA-CMI of human peripheral blood leukocytes since both MAb H52, specific to LFA-1 beta and MAb MHM24, specific to LA-1 alpha inhibited LA-CMI that retained distinct functional characteristics normally exhibited in LFA-1-dependent adhesion steps. That is, binding of cells onto tube wall required the presence of bivalent cations and metabolic energy production; it could be stimulated by phytohemagglutinin (PHA) and was partially dependent on intact cytoskeleton and temperature. MAbs specific to other adherence molecules such as ICAM-1, CD2, CD58, CD4, CD8, CD11b and LAM-1 did not affect leukocyte adherence in this model.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalJournal of Clinical and Laboratory Immunology
Volume31
Issue number3
StatePublished - Mar 1990
Externally publishedYes

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Lymphocyte Function-Associated Antigen-1
Intercellular Adhesion Molecule-1
Cellular Immunity
Leukocytes
L-Selectin
Phytohemagglutinins
Cytoskeleton
Cations
Monoclonal Antibodies
Temperature

ASJC Scopus subject areas

  • Immunology

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Involvement of lymphocyte function-associated antigen-1 (LFA-1) but not ICAM-1 in a radioactive leukocyte cell-mediated immunity (LA-CMI) assay. / Fekete, B.; Hildreth, James; Holland, J. F.; Bekesi, J. G.

In: Journal of Clinical and Laboratory Immunology, Vol. 31, No. 3, 03.1990, p. 145-149.

Research output: Contribution to journalArticle

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abstract = "A number of monoclonal antibodies (Mab) specific to human leukocyte adherence molecules were investigated for their possible function in LA-CMI assay. These data indicate that LFA-1 appears to be directly involved in LA-CMI of human peripheral blood leukocytes since both MAb H52, specific to LFA-1 beta and MAb MHM24, specific to LA-1 alpha inhibited LA-CMI that retained distinct functional characteristics normally exhibited in LFA-1-dependent adhesion steps. That is, binding of cells onto tube wall required the presence of bivalent cations and metabolic energy production; it could be stimulated by phytohemagglutinin (PHA) and was partially dependent on intact cytoskeleton and temperature. MAbs specific to other adherence molecules such as ICAM-1, CD2, CD58, CD4, CD8, CD11b and LAM-1 did not affect leukocyte adherence in this model.",
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