Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation

James Hildreth, Rimas J. Orentas

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Cell fusion (syncytium formation) is a major cytopathic effect of infection by human immunodeficiency virus (HIV) and may also represent an important mechanism of CD4+ T-cell depletion in individuals infected with HIV. Syncytium formation requires the interaction of CD4 on the surface of uninfected cells with HIV envelope glycoprotein gp120 expressed on HIV-infected cells. However, several observations suggest that molecules other than CD4 play a role in HIV-induced cell fusion. The leukocyte adhesion receptor LFA-1 is involved in a broad range of leukocyte interactions mediated by diverse receptor-ligand systems including CD4-class II major histocompatibility complex (MHC) molecules. Possible mimicry of class II MHC molecules by gp120 in its interaction with CD4 prompted an examination of the role of LFA-1 in HIV-induced cell fusion. A monoclonal antibody against LFA-1 completely inhibited HIV-induced syncytium formation. The antibody did not block binding of gp120 to CD4. This demonstrates that a molecule other than CD4 is also involved in cell fusion mediated by HIV.

Original languageEnglish (US)
Pages (from-to)1075-1078
Number of pages4
JournalScience
Volume244
Issue number4908
StatePublished - 1989
Externally publishedYes

Fingerprint

Leukocyte-Adhesion Receptors
Lymphocyte Function-Associated Antigen-1
Giant Cells
HIV
Cell Fusion
CD4 Antigens
Major Histocompatibility Complex
Glycoproteins
Leukocytes
Monoclonal Antibodies

ASJC Scopus subject areas

  • General

Cite this

Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation. / Hildreth, James; Orentas, Rimas J.

In: Science, Vol. 244, No. 4908, 1989, p. 1075-1078.

Research output: Contribution to journalArticle

@article{2d86faaa3abd4e2cb4c8cb2da39abf35,
title = "Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation",
abstract = "Cell fusion (syncytium formation) is a major cytopathic effect of infection by human immunodeficiency virus (HIV) and may also represent an important mechanism of CD4+ T-cell depletion in individuals infected with HIV. Syncytium formation requires the interaction of CD4 on the surface of uninfected cells with HIV envelope glycoprotein gp120 expressed on HIV-infected cells. However, several observations suggest that molecules other than CD4 play a role in HIV-induced cell fusion. The leukocyte adhesion receptor LFA-1 is involved in a broad range of leukocyte interactions mediated by diverse receptor-ligand systems including CD4-class II major histocompatibility complex (MHC) molecules. Possible mimicry of class II MHC molecules by gp120 in its interaction with CD4 prompted an examination of the role of LFA-1 in HIV-induced cell fusion. A monoclonal antibody against LFA-1 completely inhibited HIV-induced syncytium formation. The antibody did not block binding of gp120 to CD4. This demonstrates that a molecule other than CD4 is also involved in cell fusion mediated by HIV.",
author = "James Hildreth and Orentas, {Rimas J.}",
year = "1989",
language = "English (US)",
volume = "244",
pages = "1075--1078",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4908",

}

TY - JOUR

T1 - Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation

AU - Hildreth, James

AU - Orentas, Rimas J.

PY - 1989

Y1 - 1989

N2 - Cell fusion (syncytium formation) is a major cytopathic effect of infection by human immunodeficiency virus (HIV) and may also represent an important mechanism of CD4+ T-cell depletion in individuals infected with HIV. Syncytium formation requires the interaction of CD4 on the surface of uninfected cells with HIV envelope glycoprotein gp120 expressed on HIV-infected cells. However, several observations suggest that molecules other than CD4 play a role in HIV-induced cell fusion. The leukocyte adhesion receptor LFA-1 is involved in a broad range of leukocyte interactions mediated by diverse receptor-ligand systems including CD4-class II major histocompatibility complex (MHC) molecules. Possible mimicry of class II MHC molecules by gp120 in its interaction with CD4 prompted an examination of the role of LFA-1 in HIV-induced cell fusion. A monoclonal antibody against LFA-1 completely inhibited HIV-induced syncytium formation. The antibody did not block binding of gp120 to CD4. This demonstrates that a molecule other than CD4 is also involved in cell fusion mediated by HIV.

AB - Cell fusion (syncytium formation) is a major cytopathic effect of infection by human immunodeficiency virus (HIV) and may also represent an important mechanism of CD4+ T-cell depletion in individuals infected with HIV. Syncytium formation requires the interaction of CD4 on the surface of uninfected cells with HIV envelope glycoprotein gp120 expressed on HIV-infected cells. However, several observations suggest that molecules other than CD4 play a role in HIV-induced cell fusion. The leukocyte adhesion receptor LFA-1 is involved in a broad range of leukocyte interactions mediated by diverse receptor-ligand systems including CD4-class II major histocompatibility complex (MHC) molecules. Possible mimicry of class II MHC molecules by gp120 in its interaction with CD4 prompted an examination of the role of LFA-1 in HIV-induced cell fusion. A monoclonal antibody against LFA-1 completely inhibited HIV-induced syncytium formation. The antibody did not block binding of gp120 to CD4. This demonstrates that a molecule other than CD4 is also involved in cell fusion mediated by HIV.

UR - http://www.scopus.com/inward/record.url?scp=0024385412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024385412&partnerID=8YFLogxK

M3 - Article

VL - 244

SP - 1075

EP - 1078

JO - Science

JF - Science

SN - 0036-8075

IS - 4908

ER -