Involucrin in Laryngeal Dysplasia

A Marker for Differentiation

Michael J. Kaplan, Stacey E. Mills, Robert H. Rice, Michael E. Johns

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Involucrin is a major structural subunit of the cross-linked protein envelope that encases keratin in maturing squamous cells. Intracytoplasmic involucrin is identifiable via immunoperoxidase techniques as these cells migrate from the basal layer to the more superficial layers of the stratified epithelium. Normal squamous epithelia and mildly dysplastic epithelia show uniform staining in the suprabasal and superficial layers of the mucosa but show no staining in the basal layer. Moderate to severe dysplasias and invasive carcinomas demonstrate irregular or focal staining in all three layers. Thirtythree microscopic samples from 27 glottic laryngeal biopsy specimens were reviewed. The histochemically abnormal differentiation identified via involucrin staining correlated with accepted histologic criteria for dysplasia. Involucrin staining may provide objective information to assist the pathologist in differentiating degrees of dysplasia in laryngeal biopsy specimens.

Original languageEnglish (US)
Pages (from-to)713-716
Number of pages4
JournalArchives of Otolaryngology
Volume110
Issue number11
DOIs
StatePublished - 1984
Externally publishedYes

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Differentiation Antigens
Staining and Labeling
Epithelium
Biopsy
Keratins
Immunoenzyme Techniques
Tongue
Mucous Membrane
Epithelial Cells
involucrin
Carcinoma
Proteins

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Involucrin in Laryngeal Dysplasia : A Marker for Differentiation. / Kaplan, Michael J.; Mills, Stacey E.; Rice, Robert H.; Johns, Michael E.

In: Archives of Otolaryngology, Vol. 110, No. 11, 1984, p. 713-716.

Research output: Contribution to journalArticle

Kaplan, Michael J. ; Mills, Stacey E. ; Rice, Robert H. ; Johns, Michael E. / Involucrin in Laryngeal Dysplasia : A Marker for Differentiation. In: Archives of Otolaryngology. 1984 ; Vol. 110, No. 11. pp. 713-716.
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