Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides

Toru Takeo, Kiyoko Fukumoto, Tomoko Kondo, Yukie Haruguchi, Yumi Takeshita, Yuko Nakamuta, Shuuji Tsuchiyama, Hidetaka Yoshimoto, Norihiko Shimizu, Ming Wen Li, Kristy Kinchen, Jadine Vallelunga, Kevin C K Lloyd, Naomi Nakagata

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cold transport of epididymides from genetically modified mice is an efficient alternative to the shipment of live animals between research facilities. Mouse sperm from epididymides cold-stored for short periods can maintain viability. We previously reported that cold storage of mouse epididymides in Lifor® perfusion medium prolonged sperm motility and fertilization potential and that the sperm efficiently fertilized oocytes when reduced glutathione was added to the fertilization medium. Cryopreservation usually results in decreased sperm viability; an optimized protocol for cold storage of epididymides plus sperm cryopreservation has yet to be established. Here, we examined the motility and fertilization potential of cryopreserved, thawed (frozen-thawed) sperm from previously cold-stored mouse epididymides. We also examined the protective effect of sphingosine-1-phosphate (S1P) on sperm viability when S1P was added to the preservation medium during cold storage. We assessed viability of frozen-thawed sperm from mouse epididymides that had been cold-transported domestically or internationally and investigated whether embryos fertilized in vitro with these sperm developed normally when implanted in pseudo-pregnant mice. Our results indicate that frozen-thawed sperm from epididymides cold-stored for up to 48. h maintained high fertilization potential. Fertilization potential was reduced after cold storage for 72. h, but not if S1P was included in the cold storage medium. Live pups were born normally to recipients after in vitro fertilization using frozen-thawed sperm from cold-transported epididymides. In summary, we demonstrate an improved protocol for cold-storage of epididymides that can facilitate transport of genetically engineered-mice and preserve sperm viability after cryopreservation.

Original languageEnglish (US)
Pages (from-to)12-17
Number of pages6
JournalCryobiology
Volume68
Issue number1
DOIs
StatePublished - Feb 2014

Fingerprint

Cold storage
Epididymis
epididymis
fertilization (reproduction)
Fertilization
Spermatozoa
spermatozoa
mice
cold storage
sphingosine
viability
Cryopreservation
cryopreservation
phosphates
Glutathione
Animals
research facilities
in vitro fertilization
Sperm Motility
sperm motility

Keywords

  • Cold storage
  • Cold transport
  • Epididymides
  • In vitro fertilization
  • Motility
  • Sperm
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Takeo, T., Fukumoto, K., Kondo, T., Haruguchi, Y., Takeshita, Y., Nakamuta, Y., ... Nakagata, N. (2014). Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides. Cryobiology, 68(1), 12-17. https://doi.org/10.1016/j.cryobiol.2013.10.007

Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides. / Takeo, Toru; Fukumoto, Kiyoko; Kondo, Tomoko; Haruguchi, Yukie; Takeshita, Yumi; Nakamuta, Yuko; Tsuchiyama, Shuuji; Yoshimoto, Hidetaka; Shimizu, Norihiko; Li, Ming Wen; Kinchen, Kristy; Vallelunga, Jadine; Lloyd, Kevin C K; Nakagata, Naomi.

In: Cryobiology, Vol. 68, No. 1, 02.2014, p. 12-17.

Research output: Contribution to journalArticle

Takeo, T, Fukumoto, K, Kondo, T, Haruguchi, Y, Takeshita, Y, Nakamuta, Y, Tsuchiyama, S, Yoshimoto, H, Shimizu, N, Li, MW, Kinchen, K, Vallelunga, J, Lloyd, KCK & Nakagata, N 2014, 'Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides', Cryobiology, vol. 68, no. 1, pp. 12-17. https://doi.org/10.1016/j.cryobiol.2013.10.007
Takeo, Toru ; Fukumoto, Kiyoko ; Kondo, Tomoko ; Haruguchi, Yukie ; Takeshita, Yumi ; Nakamuta, Yuko ; Tsuchiyama, Shuuji ; Yoshimoto, Hidetaka ; Shimizu, Norihiko ; Li, Ming Wen ; Kinchen, Kristy ; Vallelunga, Jadine ; Lloyd, Kevin C K ; Nakagata, Naomi. / Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides. In: Cryobiology. 2014 ; Vol. 68, No. 1. pp. 12-17.
@article{2e89154832ab4dac9ca6f9676d64cf86,
title = "Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides",
abstract = "Cold transport of epididymides from genetically modified mice is an efficient alternative to the shipment of live animals between research facilities. Mouse sperm from epididymides cold-stored for short periods can maintain viability. We previously reported that cold storage of mouse epididymides in Lifor{\circledR} perfusion medium prolonged sperm motility and fertilization potential and that the sperm efficiently fertilized oocytes when reduced glutathione was added to the fertilization medium. Cryopreservation usually results in decreased sperm viability; an optimized protocol for cold storage of epididymides plus sperm cryopreservation has yet to be established. Here, we examined the motility and fertilization potential of cryopreserved, thawed (frozen-thawed) sperm from previously cold-stored mouse epididymides. We also examined the protective effect of sphingosine-1-phosphate (S1P) on sperm viability when S1P was added to the preservation medium during cold storage. We assessed viability of frozen-thawed sperm from mouse epididymides that had been cold-transported domestically or internationally and investigated whether embryos fertilized in vitro with these sperm developed normally when implanted in pseudo-pregnant mice. Our results indicate that frozen-thawed sperm from epididymides cold-stored for up to 48. h maintained high fertilization potential. Fertilization potential was reduced after cold storage for 72. h, but not if S1P was included in the cold storage medium. Live pups were born normally to recipients after in vitro fertilization using frozen-thawed sperm from cold-transported epididymides. In summary, we demonstrate an improved protocol for cold-storage of epididymides that can facilitate transport of genetically engineered-mice and preserve sperm viability after cryopreservation.",
keywords = "Cold storage, Cold transport, Epididymides, In vitro fertilization, Motility, Sperm, Sphingosine-1-phosphate",
author = "Toru Takeo and Kiyoko Fukumoto and Tomoko Kondo and Yukie Haruguchi and Yumi Takeshita and Yuko Nakamuta and Shuuji Tsuchiyama and Hidetaka Yoshimoto and Norihiko Shimizu and Li, {Ming Wen} and Kristy Kinchen and Jadine Vallelunga and Lloyd, {Kevin C K} and Naomi Nakagata",
year = "2014",
month = "2",
doi = "10.1016/j.cryobiol.2013.10.007",
language = "English (US)",
volume = "68",
pages = "12--17",
journal = "Cryobiology",
issn = "0011-2240",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides

AU - Takeo, Toru

AU - Fukumoto, Kiyoko

AU - Kondo, Tomoko

AU - Haruguchi, Yukie

AU - Takeshita, Yumi

AU - Nakamuta, Yuko

AU - Tsuchiyama, Shuuji

AU - Yoshimoto, Hidetaka

AU - Shimizu, Norihiko

AU - Li, Ming Wen

AU - Kinchen, Kristy

AU - Vallelunga, Jadine

AU - Lloyd, Kevin C K

AU - Nakagata, Naomi

PY - 2014/2

Y1 - 2014/2

N2 - Cold transport of epididymides from genetically modified mice is an efficient alternative to the shipment of live animals between research facilities. Mouse sperm from epididymides cold-stored for short periods can maintain viability. We previously reported that cold storage of mouse epididymides in Lifor® perfusion medium prolonged sperm motility and fertilization potential and that the sperm efficiently fertilized oocytes when reduced glutathione was added to the fertilization medium. Cryopreservation usually results in decreased sperm viability; an optimized protocol for cold storage of epididymides plus sperm cryopreservation has yet to be established. Here, we examined the motility and fertilization potential of cryopreserved, thawed (frozen-thawed) sperm from previously cold-stored mouse epididymides. We also examined the protective effect of sphingosine-1-phosphate (S1P) on sperm viability when S1P was added to the preservation medium during cold storage. We assessed viability of frozen-thawed sperm from mouse epididymides that had been cold-transported domestically or internationally and investigated whether embryos fertilized in vitro with these sperm developed normally when implanted in pseudo-pregnant mice. Our results indicate that frozen-thawed sperm from epididymides cold-stored for up to 48. h maintained high fertilization potential. Fertilization potential was reduced after cold storage for 72. h, but not if S1P was included in the cold storage medium. Live pups were born normally to recipients after in vitro fertilization using frozen-thawed sperm from cold-transported epididymides. In summary, we demonstrate an improved protocol for cold-storage of epididymides that can facilitate transport of genetically engineered-mice and preserve sperm viability after cryopreservation.

AB - Cold transport of epididymides from genetically modified mice is an efficient alternative to the shipment of live animals between research facilities. Mouse sperm from epididymides cold-stored for short periods can maintain viability. We previously reported that cold storage of mouse epididymides in Lifor® perfusion medium prolonged sperm motility and fertilization potential and that the sperm efficiently fertilized oocytes when reduced glutathione was added to the fertilization medium. Cryopreservation usually results in decreased sperm viability; an optimized protocol for cold storage of epididymides plus sperm cryopreservation has yet to be established. Here, we examined the motility and fertilization potential of cryopreserved, thawed (frozen-thawed) sperm from previously cold-stored mouse epididymides. We also examined the protective effect of sphingosine-1-phosphate (S1P) on sperm viability when S1P was added to the preservation medium during cold storage. We assessed viability of frozen-thawed sperm from mouse epididymides that had been cold-transported domestically or internationally and investigated whether embryos fertilized in vitro with these sperm developed normally when implanted in pseudo-pregnant mice. Our results indicate that frozen-thawed sperm from epididymides cold-stored for up to 48. h maintained high fertilization potential. Fertilization potential was reduced after cold storage for 72. h, but not if S1P was included in the cold storage medium. Live pups were born normally to recipients after in vitro fertilization using frozen-thawed sperm from cold-transported epididymides. In summary, we demonstrate an improved protocol for cold-storage of epididymides that can facilitate transport of genetically engineered-mice and preserve sperm viability after cryopreservation.

KW - Cold storage

KW - Cold transport

KW - Epididymides

KW - In vitro fertilization

KW - Motility

KW - Sperm

KW - Sphingosine-1-phosphate

UR - http://www.scopus.com/inward/record.url?scp=84896715833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896715833&partnerID=8YFLogxK

U2 - 10.1016/j.cryobiol.2013.10.007

DO - 10.1016/j.cryobiol.2013.10.007

M3 - Article

C2 - 24201107

AN - SCOPUS:84896715833

VL - 68

SP - 12

EP - 17

JO - Cryobiology

JF - Cryobiology

SN - 0011-2240

IS - 1

ER -