Investigation of lymphocyte gene expression for use as biomarkers for zinc status in humans

Karl B. Andree, Jihye Kim, Catherine P. Kirschke, Jeffrey Gregg, HeeYoung Y. Paik, Hyojee Joung, Leslie Woodhouse, Janet C. King, Liping Huang

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


A bioassay for zinc status in humans has been sought due to the importance of zinc, an essential trace metal, for many divergent functions in the human body; however, a sensitive bioassay for zinc status in humans is lacking. To address this issue, we established gene expression profiles of human lymphoblastoid cells treated with 0 or 30 μmol/L ZnSO 4 using microarray technology. A limited number of genes were responsive to 30 μmol/L zinc based on the analysis of Affymetrix human genome U133A GeneChips. We also examined the gene expression patterns of zinc transporters in human lymphoblastoid cells using quantitative RT-PCR analysis. ZNT1 was upregulated in lymphoblastoid cells, whereas ZIP1 was downregulated in response to the increased zinc concentrations in the culture media. To evaluate the potential applications of using both zinc transporter genes as biomarkers of zinc status, we measured the expression levels of ZIP1 and ZNT1 in the peripheral leukocytes collected from 2 different age groups of Korean women. After administration of a zinc supplement (22 mg zinc gluconate/d for 27 d), ZIP1 expression decreased by 17% (P < 0.01) and 21% (P < 0.05) in the peripheral leukocytes collected from 15 young (20-25 y) and 10 elderly (64-75 y) subjects, respectively. ZNT1 expression was not affected by taking the zinc supplement. These data suggest a potential application of ZIP1 as a biomarker of zinc status in humans.

Original languageEnglish (US)
Pages (from-to)1716-1723
Number of pages8
JournalJournal of Nutrition
Issue number7
StatePublished - Jul 2004


  • Humans
  • Microarray
  • Quantitative RT-PCR
  • Zinc supplementation
  • Zinc transporte

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science


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