Investigation of epidermotropism in canine mycosis fungoides: Expression of intercellular adhesion molecule-1 (ICAM-1) and beta-2 integrins

Thierry Olivry, Peter F Moore, Diane K. Naydan, Dimitry M. Danilenko, Verena K Affolter

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


In human mycosis fungoides (MF), interactions between LFA-1 (CD11a/CD18) and ICAM-1 (CD54) are involved in lymphocyte adhesion to keratinocytes. The purpose of this study was to evaluate the expression of ICAM-1, beta-2 integrins and class II major histocompatibility complex molecules (MHC II) on keratinocytes and infiltrating lymphocytes in canine MF. Sections of frozen skin biopsy specimens from normal dogs (n=3) and dogs with MF (n=17) were evaluated by immunohistochemistry for expression of ICAM-1, beta-2 integrins, and class II MHC molecules. Our results demonstrated that in canine MF, ICAM-1 was expressed variably on epidermal and follicular keratinocytes. The extent of keratinocyte ICAM-1 expression did not correlate with the degree of lymphocyte epithelial infiltration, nor with lymphocyte LFA-1 expression. This was especially evident in cases of Pagetoid reticulosis-like disease in which prominent lymphocyte epidermotropism was not accompanied by keratinocyte ICAM-1 expression. Keratinocyte class II MHC molecule expression did not correlate with keratinocyte ICAM-1 expression. In conclusion, in canine MF, the lack of statistically significant correlations between epithelial lymphocyte infiltration and keratinocyte ICAM-1 expression, and between keratinocyte ICAM-1 and lymphocyte LFA-1 staining, suggests that the LFA-1/ICAM-1 pathway is not the major adhesion mechanism between lymphocytes and keratinocytes. It is suspected that different ligands of the LFA-1 integrin (e.g. ICAM-2) or other adhesion molecules (e.g. CD2/LFA-3, VLA-1) might be involved in the epitheliotropism phenomenon in canine MF. These hypotheses cannot be evaluated in the dog at this time owing to the lack of specific monoclonal antibodies.

Original languageEnglish (US)
Pages (from-to)186-192
Number of pages7
JournalArchives of Dermatological Research
Issue number2
StatePublished - Feb 1995


  • Canine
  • Class II major histocompatibility complex
  • Cutaneous lymphoma
  • Dogs
  • Epitheliotropism
  • Intercellular adhesion molecule-1 (ICAM-1)
  • Keratinocytes
  • LFA-1
  • Lymphocytes
  • Mycosis fungoides

ASJC Scopus subject areas

  • Dermatology


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