Abstract
We investigated unusual crow mortality in Bangladesh during January-February 2011 at two sites. Crows of two species, Corvus splendens and C. macrorhynchos, were found sick and dead during the outbreaks. In selected crow roosts, morbidity was ~1 % and mortality was ~4 % during the investigation. Highly pathogenic avian influenza virus H5N1 clade 2.3.2.1 was isolated from dead crows. All isolates were closely related to A/duck/India/02CA10/2011 (H5N1) with 99.8 % and A/crow/Bangladesh/11rs1984-15/2011 (H5N1) virus with 99 % nucleotide sequence identity in their HA genes. The phylogenetic cluster of Bangladesh viruses suggested a common ancestor with viruses found in poultry from India, Myanmar and Nepal. Histopathological changes and immunohistochemistry staining in brain, pancreas, liver, heart, kidney, bursa of Fabricius, rectum, and cloaca were consistent with influenza virus infection. Through our limited investigation in domesticated birds near the crow roosts, we did not identify any samples that tested positive for influenza virus A/H5N1. However, environmental samples collected from live-bird markets near an outbreak site during the month of the outbreaks tested very weakly positive for influenza virus A/H5N1 in clade 2.3.2.1-specific rRT-PCR. Continuation of surveillance in wild and domestic birds may identify evolution of new avian influenza virus and associated public-health risks.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 509-518 |
| Number of pages | 10 |
| Journal | Archives of Virology |
| Volume | 159 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2014 |
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- Virology
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Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh. / Khan, Salah Uddin; Berman, LaShondra S.; Haider, Najmul; Gerloff, Nancy; Rahman, Md Z.; Shu, Bo; Rahman, Mustafizur; Dey, Tapan Kumar; Davis, Todd C.; Das, Bidhan Chandra; Balish, Amanda; Islam, Ausraful; Teifke, Jens P.; Zeidner, Nord; Lindstrom, Steven; Klimov, Alexander; Donis, Ruben O.; Luby, Stephen P.; Shivaprasad, H L; Mikolon, Andrea B.
In: Archives of Virology, Vol. 159, No. 3, 03.2014, p. 509-518.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh
AU - Khan, Salah Uddin
AU - Berman, LaShondra S.
AU - Haider, Najmul
AU - Gerloff, Nancy
AU - Rahman, Md Z.
AU - Shu, Bo
AU - Rahman, Mustafizur
AU - Dey, Tapan Kumar
AU - Davis, Todd C.
AU - Das, Bidhan Chandra
AU - Balish, Amanda
AU - Islam, Ausraful
AU - Teifke, Jens P.
AU - Zeidner, Nord
AU - Lindstrom, Steven
AU - Klimov, Alexander
AU - Donis, Ruben O.
AU - Luby, Stephen P.
AU - Shivaprasad, H L
AU - Mikolon, Andrea B.
PY - 2014/3
Y1 - 2014/3
N2 - We investigated unusual crow mortality in Bangladesh during January-February 2011 at two sites. Crows of two species, Corvus splendens and C. macrorhynchos, were found sick and dead during the outbreaks. In selected crow roosts, morbidity was ~1 % and mortality was ~4 % during the investigation. Highly pathogenic avian influenza virus H5N1 clade 2.3.2.1 was isolated from dead crows. All isolates were closely related to A/duck/India/02CA10/2011 (H5N1) with 99.8 % and A/crow/Bangladesh/11rs1984-15/2011 (H5N1) virus with 99 % nucleotide sequence identity in their HA genes. The phylogenetic cluster of Bangladesh viruses suggested a common ancestor with viruses found in poultry from India, Myanmar and Nepal. Histopathological changes and immunohistochemistry staining in brain, pancreas, liver, heart, kidney, bursa of Fabricius, rectum, and cloaca were consistent with influenza virus infection. Through our limited investigation in domesticated birds near the crow roosts, we did not identify any samples that tested positive for influenza virus A/H5N1. However, environmental samples collected from live-bird markets near an outbreak site during the month of the outbreaks tested very weakly positive for influenza virus A/H5N1 in clade 2.3.2.1-specific rRT-PCR. Continuation of surveillance in wild and domestic birds may identify evolution of new avian influenza virus and associated public-health risks.
AB - We investigated unusual crow mortality in Bangladesh during January-February 2011 at two sites. Crows of two species, Corvus splendens and C. macrorhynchos, were found sick and dead during the outbreaks. In selected crow roosts, morbidity was ~1 % and mortality was ~4 % during the investigation. Highly pathogenic avian influenza virus H5N1 clade 2.3.2.1 was isolated from dead crows. All isolates were closely related to A/duck/India/02CA10/2011 (H5N1) with 99.8 % and A/crow/Bangladesh/11rs1984-15/2011 (H5N1) virus with 99 % nucleotide sequence identity in their HA genes. The phylogenetic cluster of Bangladesh viruses suggested a common ancestor with viruses found in poultry from India, Myanmar and Nepal. Histopathological changes and immunohistochemistry staining in brain, pancreas, liver, heart, kidney, bursa of Fabricius, rectum, and cloaca were consistent with influenza virus infection. Through our limited investigation in domesticated birds near the crow roosts, we did not identify any samples that tested positive for influenza virus A/H5N1. However, environmental samples collected from live-bird markets near an outbreak site during the month of the outbreaks tested very weakly positive for influenza virus A/H5N1 in clade 2.3.2.1-specific rRT-PCR. Continuation of surveillance in wild and domestic birds may identify evolution of new avian influenza virus and associated public-health risks.
UR - http://www.scopus.com/inward/record.url?scp=84896881322&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896881322&partnerID=8YFLogxK
U2 - 10.1007/s00705-013-1842-0
DO - 10.1007/s00705-013-1842-0
M3 - Article
C2 - 24081824
AN - SCOPUS:84896881322
VL - 159
SP - 509
EP - 518
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 3
ER -