Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh

Salah Uddin Khan, LaShondra S. Berman, Najmul Haider, Nancy Gerloff, Md Z. Rahman, Bo Shu, Mustafizur Rahman, Tapan Kumar Dey, Todd C. Davis, Bidhan Chandra Das, Amanda Balish, Ausraful Islam, Jens P. Teifke, Nord Zeidner, Steven Lindstrom, Alexander Klimov, Ruben O. Donis, Stephen P. Luby, H L Shivaprasad, Andrea B. Mikolon

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Abstract

We investigated unusual crow mortality in Bangladesh during January-February 2011 at two sites. Crows of two species, Corvus splendens and C. macrorhynchos, were found sick and dead during the outbreaks. In selected crow roosts, morbidity was ~1 % and mortality was ~4 % during the investigation. Highly pathogenic avian influenza virus H5N1 clade 2.3.2.1 was isolated from dead crows. All isolates were closely related to A/duck/India/02CA10/2011 (H5N1) with 99.8 % and A/crow/Bangladesh/11rs1984-15/2011 (H5N1) virus with 99 % nucleotide sequence identity in their HA genes. The phylogenetic cluster of Bangladesh viruses suggested a common ancestor with viruses found in poultry from India, Myanmar and Nepal. Histopathological changes and immunohistochemistry staining in brain, pancreas, liver, heart, kidney, bursa of Fabricius, rectum, and cloaca were consistent with influenza virus infection. Through our limited investigation in domesticated birds near the crow roosts, we did not identify any samples that tested positive for influenza virus A/H5N1. However, environmental samples collected from live-bird markets near an outbreak site during the month of the outbreaks tested very weakly positive for influenza virus A/H5N1 in clade 2.3.2.1-specific rRT-PCR. Continuation of surveillance in wild and domestic birds may identify evolution of new avian influenza virus and associated public-health risks.

Original languageEnglish (US)
Pages (from-to)509-518
Number of pages10
JournalArchives of Virology
Volume159
Issue number3
DOIs
StatePublished - Mar 2014

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Crows
Influenza in Birds
Bangladesh
Orthomyxoviridae
Birds
Disease Outbreaks
Influenza A virus
India
Bursa of Fabricius
Cloaca
Myanmar
H5N1 Subtype Influenza A Virus
Viruses
Nepal
Ducks
Mortality
Virus Diseases
Poultry
Rectum
Pancreas

ASJC Scopus subject areas

  • Virology

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Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh. / Khan, Salah Uddin; Berman, LaShondra S.; Haider, Najmul; Gerloff, Nancy; Rahman, Md Z.; Shu, Bo; Rahman, Mustafizur; Dey, Tapan Kumar; Davis, Todd C.; Das, Bidhan Chandra; Balish, Amanda; Islam, Ausraful; Teifke, Jens P.; Zeidner, Nord; Lindstrom, Steven; Klimov, Alexander; Donis, Ruben O.; Luby, Stephen P.; Shivaprasad, H L; Mikolon, Andrea B.

In: Archives of Virology, Vol. 159, No. 3, 03.2014, p. 509-518.

Research output: Contribution to journalArticle

Khan, SU, Berman, LS, Haider, N, Gerloff, N, Rahman, MZ, Shu, B, Rahman, M, Dey, TK, Davis, TC, Das, BC, Balish, A, Islam, A, Teifke, JP, Zeidner, N, Lindstrom, S, Klimov, A, Donis, RO, Luby, SP, Shivaprasad, HL & Mikolon, AB 2014, 'Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh', Archives of Virology, vol. 159, no. 3, pp. 509-518. https://doi.org/10.1007/s00705-013-1842-0
Khan, Salah Uddin ; Berman, LaShondra S. ; Haider, Najmul ; Gerloff, Nancy ; Rahman, Md Z. ; Shu, Bo ; Rahman, Mustafizur ; Dey, Tapan Kumar ; Davis, Todd C. ; Das, Bidhan Chandra ; Balish, Amanda ; Islam, Ausraful ; Teifke, Jens P. ; Zeidner, Nord ; Lindstrom, Steven ; Klimov, Alexander ; Donis, Ruben O. ; Luby, Stephen P. ; Shivaprasad, H L ; Mikolon, Andrea B. / Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh. In: Archives of Virology. 2014 ; Vol. 159, No. 3. pp. 509-518.
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abstract = "We investigated unusual crow mortality in Bangladesh during January-February 2011 at two sites. Crows of two species, Corvus splendens and C. macrorhynchos, were found sick and dead during the outbreaks. In selected crow roosts, morbidity was ~1 {\%} and mortality was ~4 {\%} during the investigation. Highly pathogenic avian influenza virus H5N1 clade 2.3.2.1 was isolated from dead crows. All isolates were closely related to A/duck/India/02CA10/2011 (H5N1) with 99.8 {\%} and A/crow/Bangladesh/11rs1984-15/2011 (H5N1) virus with 99 {\%} nucleotide sequence identity in their HA genes. The phylogenetic cluster of Bangladesh viruses suggested a common ancestor with viruses found in poultry from India, Myanmar and Nepal. Histopathological changes and immunohistochemistry staining in brain, pancreas, liver, heart, kidney, bursa of Fabricius, rectum, and cloaca were consistent with influenza virus infection. Through our limited investigation in domesticated birds near the crow roosts, we did not identify any samples that tested positive for influenza virus A/H5N1. However, environmental samples collected from live-bird markets near an outbreak site during the month of the outbreaks tested very weakly positive for influenza virus A/H5N1 in clade 2.3.2.1-specific rRT-PCR. Continuation of surveillance in wild and domestic birds may identify evolution of new avian influenza virus and associated public-health risks.",
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AU - Berman, LaShondra S.

AU - Haider, Najmul

AU - Gerloff, Nancy

AU - Rahman, Md Z.

AU - Shu, Bo

AU - Rahman, Mustafizur

AU - Dey, Tapan Kumar

AU - Davis, Todd C.

AU - Das, Bidhan Chandra

AU - Balish, Amanda

AU - Islam, Ausraful

AU - Teifke, Jens P.

AU - Zeidner, Nord

AU - Lindstrom, Steven

AU - Klimov, Alexander

AU - Donis, Ruben O.

AU - Luby, Stephen P.

AU - Shivaprasad, H L

AU - Mikolon, Andrea B.

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N2 - We investigated unusual crow mortality in Bangladesh during January-February 2011 at two sites. Crows of two species, Corvus splendens and C. macrorhynchos, were found sick and dead during the outbreaks. In selected crow roosts, morbidity was ~1 % and mortality was ~4 % during the investigation. Highly pathogenic avian influenza virus H5N1 clade 2.3.2.1 was isolated from dead crows. All isolates were closely related to A/duck/India/02CA10/2011 (H5N1) with 99.8 % and A/crow/Bangladesh/11rs1984-15/2011 (H5N1) virus with 99 % nucleotide sequence identity in their HA genes. The phylogenetic cluster of Bangladesh viruses suggested a common ancestor with viruses found in poultry from India, Myanmar and Nepal. Histopathological changes and immunohistochemistry staining in brain, pancreas, liver, heart, kidney, bursa of Fabricius, rectum, and cloaca were consistent with influenza virus infection. Through our limited investigation in domesticated birds near the crow roosts, we did not identify any samples that tested positive for influenza virus A/H5N1. However, environmental samples collected from live-bird markets near an outbreak site during the month of the outbreaks tested very weakly positive for influenza virus A/H5N1 in clade 2.3.2.1-specific rRT-PCR. Continuation of surveillance in wild and domestic birds may identify evolution of new avian influenza virus and associated public-health risks.

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