Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh

Salah Uddin Khan, LaShondra S. Berman, Najmul Haider, Nancy Gerloff, Md Z. Rahman, Bo Shu, Mustafizur Rahman, Tapan Kumar Dey, Todd C. Davis, Bidhan Chandra Das, Amanda Balish, Ausraful Islam, Jens P. Teifke, Nord Zeidner, Steven Lindstrom, Alexander Klimov, Ruben O. Donis, Stephen P. Luby, H L Shivaprasad, Andrea B. Mikolon

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We investigated unusual crow mortality in Bangladesh during January-February 2011 at two sites. Crows of two species, Corvus splendens and C. macrorhynchos, were found sick and dead during the outbreaks. In selected crow roosts, morbidity was ~1 % and mortality was ~4 % during the investigation. Highly pathogenic avian influenza virus H5N1 clade 2.3.2.1 was isolated from dead crows. All isolates were closely related to A/duck/India/02CA10/2011 (H5N1) with 99.8 % and A/crow/Bangladesh/11rs1984-15/2011 (H5N1) virus with 99 % nucleotide sequence identity in their HA genes. The phylogenetic cluster of Bangladesh viruses suggested a common ancestor with viruses found in poultry from India, Myanmar and Nepal. Histopathological changes and immunohistochemistry staining in brain, pancreas, liver, heart, kidney, bursa of Fabricius, rectum, and cloaca were consistent with influenza virus infection. Through our limited investigation in domesticated birds near the crow roosts, we did not identify any samples that tested positive for influenza virus A/H5N1. However, environmental samples collected from live-bird markets near an outbreak site during the month of the outbreaks tested very weakly positive for influenza virus A/H5N1 in clade 2.3.2.1-specific rRT-PCR. Continuation of surveillance in wild and domestic birds may identify evolution of new avian influenza virus and associated public-health risks.

Original languageEnglish (US)
Pages (from-to)509-518
Number of pages10
JournalArchives of Virology
Volume159
Issue number3
DOIs
StatePublished - Mar 2014

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh'. Together they form a unique fingerprint.

  • Cite this

    Khan, S. U., Berman, L. S., Haider, N., Gerloff, N., Rahman, M. Z., Shu, B., Rahman, M., Dey, T. K., Davis, T. C., Das, B. C., Balish, A., Islam, A., Teifke, J. P., Zeidner, N., Lindstrom, S., Klimov, A., Donis, R. O., Luby, S. P., Shivaprasad, H. L., & Mikolon, A. B. (2014). Investigating a crow die-off in January-February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh. Archives of Virology, 159(3), 509-518. https://doi.org/10.1007/s00705-013-1842-0