Inverse zonation of hepatocyte transduction with AAV vectors between mice and non-human primates

Peter Bell, Lili Wang, Guangping Gao, Mark E. Haskins, Alice F Tarantal, Robert J. McCarter, Yanqing Zhu, Hongwei Yu, James M. Wilson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Gene transfer vectors based on adeno-associated virus 8 (AAV8) are highly efficient in liver transduction and can be easily administered by intravenous injection. In mice, AAV8 transduces predominantly hepatocytes near central veins and yields lower transduction levels in hepatocytes in periportal regions. This transduction bias has important implications for gene therapy that aims to correct metabolic liver enzymes because metabolic zonation along the porto-central axis requires the expression of therapeutic proteins within the zone where they are normally localized. In the present study we compared the expression pattern of AAV8 expressing green fluorescent protein (GFP) in liver between mice, dogs, and non-human primates. We confirmed the pericentral dominance in transgene expression in mice with AAV8 when the liver-specific thyroid hormone-binding globulin (TBG) promoter was used but also observed the same expression pattern with the ubiquitous chicken β-actin (CB) and cytomegalovirus (CMV) promoters, suggesting that transduction zonation is not caused by promoter specificity. Predominantly pericentral expression was also found in dogs injected with AAV8. In contrast, in cynomolgus and rhesus macaques the expression pattern from AAV vectors was reversed, i.e. transgene expression was most intense around portal areas and less intense or absent around central veins. Infant rhesus macaques as well as newborn mice injected with AAV8 however showed a random distribution of transgene expression with neither portal nor central transduction bias. Based on the data in monkeys, adult humans treated with AAV vectors are predicted to also express transgenes predominantly in periportal regions whereas infants are likely to show a uniform transduction pattern in liver.

Original languageEnglish (US)
Pages (from-to)395-403
Number of pages9
JournalMolecular Genetics and Metabolism
Volume104
Issue number3
DOIs
StatePublished - Nov 2011

Fingerprint

Dependovirus
Viruses
Primates
Hepatocytes
Liver
Transgenes
Macaca mulatta
Veins
Dogs
Gene transfer
Gene therapy
Globulins
Green Fluorescent Proteins
Cytomegalovirus
Thyroid Hormones
Intravenous Injections
Genetic Therapy
Haplorhini
Actins
Chickens

Keywords

  • AAV
  • Animal models
  • Gene therapy
  • Liver

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Inverse zonation of hepatocyte transduction with AAV vectors between mice and non-human primates. / Bell, Peter; Wang, Lili; Gao, Guangping; Haskins, Mark E.; Tarantal, Alice F; McCarter, Robert J.; Zhu, Yanqing; Yu, Hongwei; Wilson, James M.

In: Molecular Genetics and Metabolism, Vol. 104, No. 3, 11.2011, p. 395-403.

Research output: Contribution to journalArticle

Bell, Peter ; Wang, Lili ; Gao, Guangping ; Haskins, Mark E. ; Tarantal, Alice F ; McCarter, Robert J. ; Zhu, Yanqing ; Yu, Hongwei ; Wilson, James M. / Inverse zonation of hepatocyte transduction with AAV vectors between mice and non-human primates. In: Molecular Genetics and Metabolism. 2011 ; Vol. 104, No. 3. pp. 395-403.
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