Introduction to the purification of antibodies.

Ana Grodzki, Elsa Berenstein

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Antibodies are a powerful and essential tool in scientific laboratories being used in an array of applications such as immuno-histochemistry, immunobloting, immunoprecipitation and enzyme-linked immunosorbent assays (ELISA). The different sources for antibodies include polyclonal antisera from immunized animals and monoclonal antibodies from cells in culture or from ascites in animals. Both polyclonal and monoclonal antibodies have their advantages, and or disadvantages, but in general the production of monoclonal antibodies is more time consuming and requires tissue culture facilities and skills. The use of either monoclonal or polyclonal antibodies in some of the applications may require that the antibody is in a purified form. They can be purified by a variety of methods described in the next few chapters. The availability of commercially available kits primarily designed for the purification of IgG and IgM classes of antibodies derived from all common animal species should also be mentioned.

Original languageEnglish (US)
Pages (from-to)11-13
Number of pages3
JournalMethods in molecular biology (Clifton, N.J.)
Volume588
StatePublished - Mar 4 2010
Externally publishedYes

Fingerprint

Antibodies
Monoclonal Antibodies
Immunoglobulin Isotypes
Immunoprecipitation
Ascites
Immunoglobulin M
Immune Sera
Cell Culture Techniques
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Introduction to the purification of antibodies. / Grodzki, Ana; Berenstein, Elsa.

In: Methods in molecular biology (Clifton, N.J.), Vol. 588, 04.03.2010, p. 11-13.

Research output: Contribution to journalArticle

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