Abstract
A dual-radioisotope method was used to simultaneously study whole-body manganese retention from a chicken liver based meal intrinsically labeled with 54Mn and extrinsically labeled with 52Mn. Manganese retention was monitored in a sensitive whole-body counter during approximately 30 d in six young adult women. Both radioisotopes were retained to a similar degree and excreted at identical rates. Retention at d 5 was 14.4 ± 10.3 and 14.0 ± 9.9% while retention at d 10 was 5.0 ± 3.1 and 5.0 ± 3.0% (X̄ ± SD) for 54Mn and 52Mn, respectively. From these results we conclude that the intrinsic and extrinsic Mn isotopes did form a common pool before absorption. The results can therefore be regarded as a direct validation of the use of extrinsic labeling for studies of Mn retention for estimating Mn absorption in man.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1517-1521 |
| Number of pages | 5 |
| Journal | Journal of Nutrition |
| Volume | 118 |
| Issue number | 12 |
| State | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
Cite this
Intrinsic and extrinsic labeling for studies of manganese absorption in humans. / Davidsson, L.; Cederblad, A.; Hagebo, E.; Lonnerdal, B.; Sandstrom, B.
In: Journal of Nutrition, Vol. 118, No. 12, 1988, p. 1517-1521.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intrinsic and extrinsic labeling for studies of manganese absorption in humans
AU - Davidsson, L.
AU - Cederblad, A.
AU - Hagebo, E.
AU - Lonnerdal, B.
AU - Sandstrom, B.
PY - 1988
Y1 - 1988
N2 - A dual-radioisotope method was used to simultaneously study whole-body manganese retention from a chicken liver based meal intrinsically labeled with 54Mn and extrinsically labeled with 52Mn. Manganese retention was monitored in a sensitive whole-body counter during approximately 30 d in six young adult women. Both radioisotopes were retained to a similar degree and excreted at identical rates. Retention at d 5 was 14.4 ± 10.3 and 14.0 ± 9.9% while retention at d 10 was 5.0 ± 3.1 and 5.0 ± 3.0% (X̄ ± SD) for 54Mn and 52Mn, respectively. From these results we conclude that the intrinsic and extrinsic Mn isotopes did form a common pool before absorption. The results can therefore be regarded as a direct validation of the use of extrinsic labeling for studies of Mn retention for estimating Mn absorption in man.
AB - A dual-radioisotope method was used to simultaneously study whole-body manganese retention from a chicken liver based meal intrinsically labeled with 54Mn and extrinsically labeled with 52Mn. Manganese retention was monitored in a sensitive whole-body counter during approximately 30 d in six young adult women. Both radioisotopes were retained to a similar degree and excreted at identical rates. Retention at d 5 was 14.4 ± 10.3 and 14.0 ± 9.9% while retention at d 10 was 5.0 ± 3.1 and 5.0 ± 3.0% (X̄ ± SD) for 54Mn and 52Mn, respectively. From these results we conclude that the intrinsic and extrinsic Mn isotopes did form a common pool before absorption. The results can therefore be regarded as a direct validation of the use of extrinsic labeling for studies of Mn retention for estimating Mn absorption in man.
UR - http://www.scopus.com/inward/record.url?scp=0024232103&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024232103&partnerID=8YFLogxK
M3 - Article
C2 - 3145332
AN - SCOPUS:0024232103
VL - 118
SP - 1517
EP - 1521
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 12
ER -