Intravenous and subcutaneous pharmacokinetics of florfenicol in sheep

V Michael Lane, S. Wetzlich, A. Clifford, I. Taylor, A. L. Craigmill

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Pharmacokinetic parameters of florfenicol were determined in 10 adult sheep (five wethers and five ewes) after a single 40 mg/kg intravenous (i.v.) dose, and three daily subcutaneous (s.c.) doses of 40 mg/kg of a commercial preparation (Nuflor®). The concentration of florfenicol in serum samples was assayed using a proprietary HPLC assay method, and pharmacokinetic parameters derived for individual animal data by each route using compartmental and noncompartmental approaches. Two animals (one male and one female) were excluded due to observed i.v. dosing problems, and a biexponential model was found to fit the i.v. data well for six of the other eight animals. Data from two males showed prolonged low concentrations of florfenicol in serum and were better fit by a three-compartment model. The mean ± SD for the half-lives of the distribution and elimination phases for the six sheep best fit with a two-compartment model were 0.069 ± 0.018 and 1.01 ± 0.09 h respectively, and for the V d(ss) and clearances were 0.503 ± 0.035 L/kg and 366 ± 53 mL/h/kg respectively. The data collected during the s.c. multiple dose study were analyzed using noncompartmental methods only. The bioavailability (F%) after s.c. dosing was calculated in three ways to compare estimation methods as steady-state had not been reached and single dose s.c. data were not obtained past 24 h. Using the AUC 0-24 and AUC 0 → ∞ from the first dose, the F% values averaged 27 and 40% respectively. Using the AUC 0 → ∞ for all doses, the F% was 65%. Calculations of the mean time during which the serum concentration exceeded 0.5 and 1.0 μg/mL were 105 ± 3.9 and 74.7 ± 12.2 h respectively.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalJournal of Veterinary Pharmacology and Therapeutics
Volume27
Issue number4
DOIs
StatePublished - Aug 2004

Fingerprint

florfenicol
pharmacokinetics
Area Under Curve
Sheep
Pharmacokinetics
sheep
dosage
Serum
Biological Availability
High Pressure Liquid Chromatography
animals
half life
bioavailability
ewes
methodology
assays

ASJC Scopus subject areas

  • Pharmacology
  • veterinary(all)

Cite this

Intravenous and subcutaneous pharmacokinetics of florfenicol in sheep. / Lane, V Michael; Wetzlich, S.; Clifford, A.; Taylor, I.; Craigmill, A. L.

In: Journal of Veterinary Pharmacology and Therapeutics, Vol. 27, No. 4, 08.2004, p. 191-196.

Research output: Contribution to journalArticle

Lane, V Michael ; Wetzlich, S. ; Clifford, A. ; Taylor, I. ; Craigmill, A. L. / Intravenous and subcutaneous pharmacokinetics of florfenicol in sheep. In: Journal of Veterinary Pharmacology and Therapeutics. 2004 ; Vol. 27, No. 4. pp. 191-196.
@article{601268a94dff4898b6d3dc0950cee157,
title = "Intravenous and subcutaneous pharmacokinetics of florfenicol in sheep",
abstract = "Pharmacokinetic parameters of florfenicol were determined in 10 adult sheep (five wethers and five ewes) after a single 40 mg/kg intravenous (i.v.) dose, and three daily subcutaneous (s.c.) doses of 40 mg/kg of a commercial preparation (Nuflor{\circledR}). The concentration of florfenicol in serum samples was assayed using a proprietary HPLC assay method, and pharmacokinetic parameters derived for individual animal data by each route using compartmental and noncompartmental approaches. Two animals (one male and one female) were excluded due to observed i.v. dosing problems, and a biexponential model was found to fit the i.v. data well for six of the other eight animals. Data from two males showed prolonged low concentrations of florfenicol in serum and were better fit by a three-compartment model. The mean ± SD for the half-lives of the distribution and elimination phases for the six sheep best fit with a two-compartment model were 0.069 ± 0.018 and 1.01 ± 0.09 h respectively, and for the V d(ss) and clearances were 0.503 ± 0.035 L/kg and 366 ± 53 mL/h/kg respectively. The data collected during the s.c. multiple dose study were analyzed using noncompartmental methods only. The bioavailability (F{\%}) after s.c. dosing was calculated in three ways to compare estimation methods as steady-state had not been reached and single dose s.c. data were not obtained past 24 h. Using the AUC 0-24 and AUC 0 → ∞ from the first dose, the F{\%} values averaged 27 and 40{\%} respectively. Using the AUC 0 → ∞ for all doses, the F{\%} was 65{\%}. Calculations of the mean time during which the serum concentration exceeded 0.5 and 1.0 μg/mL were 105 ± 3.9 and 74.7 ± 12.2 h respectively.",
author = "Lane, {V Michael} and S. Wetzlich and A. Clifford and I. Taylor and Craigmill, {A. L.}",
year = "2004",
month = "8",
doi = "10.1111/j.1365-2885.2004.00580.x",
language = "English (US)",
volume = "27",
pages = "191--196",
journal = "Journal of Veterinary Pharmacology and Therapeutics",
issn = "0140-7783",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Intravenous and subcutaneous pharmacokinetics of florfenicol in sheep

AU - Lane, V Michael

AU - Wetzlich, S.

AU - Clifford, A.

AU - Taylor, I.

AU - Craigmill, A. L.

PY - 2004/8

Y1 - 2004/8

N2 - Pharmacokinetic parameters of florfenicol were determined in 10 adult sheep (five wethers and five ewes) after a single 40 mg/kg intravenous (i.v.) dose, and three daily subcutaneous (s.c.) doses of 40 mg/kg of a commercial preparation (Nuflor®). The concentration of florfenicol in serum samples was assayed using a proprietary HPLC assay method, and pharmacokinetic parameters derived for individual animal data by each route using compartmental and noncompartmental approaches. Two animals (one male and one female) were excluded due to observed i.v. dosing problems, and a biexponential model was found to fit the i.v. data well for six of the other eight animals. Data from two males showed prolonged low concentrations of florfenicol in serum and were better fit by a three-compartment model. The mean ± SD for the half-lives of the distribution and elimination phases for the six sheep best fit with a two-compartment model were 0.069 ± 0.018 and 1.01 ± 0.09 h respectively, and for the V d(ss) and clearances were 0.503 ± 0.035 L/kg and 366 ± 53 mL/h/kg respectively. The data collected during the s.c. multiple dose study were analyzed using noncompartmental methods only. The bioavailability (F%) after s.c. dosing was calculated in three ways to compare estimation methods as steady-state had not been reached and single dose s.c. data were not obtained past 24 h. Using the AUC 0-24 and AUC 0 → ∞ from the first dose, the F% values averaged 27 and 40% respectively. Using the AUC 0 → ∞ for all doses, the F% was 65%. Calculations of the mean time during which the serum concentration exceeded 0.5 and 1.0 μg/mL were 105 ± 3.9 and 74.7 ± 12.2 h respectively.

AB - Pharmacokinetic parameters of florfenicol were determined in 10 adult sheep (five wethers and five ewes) after a single 40 mg/kg intravenous (i.v.) dose, and three daily subcutaneous (s.c.) doses of 40 mg/kg of a commercial preparation (Nuflor®). The concentration of florfenicol in serum samples was assayed using a proprietary HPLC assay method, and pharmacokinetic parameters derived for individual animal data by each route using compartmental and noncompartmental approaches. Two animals (one male and one female) were excluded due to observed i.v. dosing problems, and a biexponential model was found to fit the i.v. data well for six of the other eight animals. Data from two males showed prolonged low concentrations of florfenicol in serum and were better fit by a three-compartment model. The mean ± SD for the half-lives of the distribution and elimination phases for the six sheep best fit with a two-compartment model were 0.069 ± 0.018 and 1.01 ± 0.09 h respectively, and for the V d(ss) and clearances were 0.503 ± 0.035 L/kg and 366 ± 53 mL/h/kg respectively. The data collected during the s.c. multiple dose study were analyzed using noncompartmental methods only. The bioavailability (F%) after s.c. dosing was calculated in three ways to compare estimation methods as steady-state had not been reached and single dose s.c. data were not obtained past 24 h. Using the AUC 0-24 and AUC 0 → ∞ from the first dose, the F% values averaged 27 and 40% respectively. Using the AUC 0 → ∞ for all doses, the F% was 65%. Calculations of the mean time during which the serum concentration exceeded 0.5 and 1.0 μg/mL were 105 ± 3.9 and 74.7 ± 12.2 h respectively.

UR - http://www.scopus.com/inward/record.url?scp=4344601050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344601050&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2885.2004.00580.x

DO - 10.1111/j.1365-2885.2004.00580.x

M3 - Article

C2 - 15305846

AN - SCOPUS:4344601050

VL - 27

SP - 191

EP - 196

JO - Journal of Veterinary Pharmacology and Therapeutics

JF - Journal of Veterinary Pharmacology and Therapeutics

SN - 0140-7783

IS - 4

ER -