Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy

Pramod V. Butte, Qiyin Fang, Javier A. Jo, William H. Yong, Brian K. Pikul, Keith L. Black, Laura Marcu

Research output: Contribution to journalArticle

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Abstract

The goal of this study is to determine the potential of timeresolved laser-induced fluorescence spectroscopy (TR-LIFS) as an adjunctive tool for delineation of brain tumor from surrounding normal tissue in order to assist the neurosurgeon in near-complete tumor excision. A time-domain TR-LIFS prototype apparatus (gated photomultiplier detection, fast digitizer) was used for recording tissue autofluorescence in normal cortex (NC), normal white matter (NWM), and various grades of gliomas intraoperatively. Tissue fluorescence was induced with a pulsed nitrogen laser (337 nm, 700 ps), and the intensity decay profiles were recorded in the 360-to 550-nm spectral range (10-nm interval). Histopathological analysis (hematoxylin & eosin) of the biopsy samples taken from the site of TR-LIFS measurements was used for validation of spectroscopic results. Preliminary results on 17 patients demonstrate that normal cortex (N=16) and normal white matter (N=3) show two peaks of fluorescence emission at 390 nm (lifetime = 1.8±0.3 ns) and 460 nm (lifetime = 0.8±0.1 ns). The 390-nm emission peak is absent in low-grade glioma (N=5; lifetime=1.1 ns) and reduced in high-grade glioma (N=9; lifetime = 1.7±0.4 ns). The emission characteristics at 460 nm in all tissues correlated with the nicotinamide adenine dinucleotide fluorescence (peak: 440 to 460 nm; lifetime: 0.8 to 1.0 ns). These findings demonstrate the potential of using TR-LIFS as a tool for enhanced delineation of brain tumors during surgery. In addition, this study evaluates similarities and differences between TR-LIFS signatures of brain tumors obtained in vivo and those previously reported in ex vivo brain tumor specimens.

Original languageEnglish (US)
Article number027008
JournalJournal of Biomedical Optics
Volume15
Issue number2
DOIs
StatePublished - 2010

Fingerprint

delineation
Fluorescence spectroscopy
laser induced fluorescence
brain
Tumors
Brain
tumors
life (durability)
fluorescence
Lasers
grade
Tissue
spectroscopy
cortexes
Fluorescence
nicotinamide
nitrogen lasers
adenines
analog to digital converters
surgery

Keywords

  • Autofluorescence
  • Fluorescence
  • Glioma
  • Intraoperative
  • Spectroscopy
  • Time resolved

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Butte, P. V., Fang, Q., Jo, J. A., Yong, W. H., Pikul, B. K., Black, K. L., & Marcu, L. (2010). Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy. Journal of Biomedical Optics, 15(2), [027008]. https://doi.org/10.1117/1.3374049

Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy. / Butte, Pramod V.; Fang, Qiyin; Jo, Javier A.; Yong, William H.; Pikul, Brian K.; Black, Keith L.; Marcu, Laura.

In: Journal of Biomedical Optics, Vol. 15, No. 2, 027008, 2010.

Research output: Contribution to journalArticle

Butte, PV, Fang, Q, Jo, JA, Yong, WH, Pikul, BK, Black, KL & Marcu, L 2010, 'Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy', Journal of Biomedical Optics, vol. 15, no. 2, 027008. https://doi.org/10.1117/1.3374049
Butte, Pramod V. ; Fang, Qiyin ; Jo, Javier A. ; Yong, William H. ; Pikul, Brian K. ; Black, Keith L. ; Marcu, Laura. / Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy. In: Journal of Biomedical Optics. 2010 ; Vol. 15, No. 2.
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