Intragastric and intracoelomic injection challenge models of tilapia lake virus infection in Nile tilapia (Oreochromis niloticus L.) fingerlings

Felipe Pierezan, Susan Yun, Win Surachetpong, Esteban Soto Martinez

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To gain a better understanding of the pathogenesis of tilapia lake virus (TiLV) infections in Nile tilapia (Oreochromis niloticus), fingerlings were challenged with a single dose of 1 × 104 TCID50/fish of TiLV utilizing intracoelomic/intraperitoneal (ICch) or intragastric (IGch) routes. Acute mortalities were present in both groups, reaching 70 and 40% in ICch and IGch after 10 days, respectively. Challenged fish presented erratic swimming, lethargy, anorexia, exophthalmia and cutaneous petechiae and ecchymoses. Histological changes in challenged groups included syncytial formation, intracytoplasmic inclusion bodies and multifocal hepatocellular degeneration and necrosis. In addition, multifocal areas of mild proliferation of glial cells and lymphocytic perivascular cuffing were observed in the brain of exposed challenged groups. TiLV RNA was detected in gills and faeces of challenged fish using quantitative reverse transcriptase–PCR, as well as in the tank water holding challenged fish. Moreover, TiLV RNA was detected in scrolls obtained from formalin-fixed paraffin-embedded tissue blocks from challenged fish. Results from this study suggest that IG methods represent an additional method to study the pathogenesis of the disease in this species, as it results in infection and diseases as in naturally occurring cases and does not bypass important mucosal immune responses as injectable routes do.

Original languageEnglish (US)
JournalJournal of fish diseases
DOIs
StatePublished - Jan 1 2019

Fingerprint

Tilapia (Cichlidae)
Tilapia
Cichlids
Virus Diseases
fingerlings
Lakes
Oreochromis niloticus
virus
Fishes
injection
tilapia (common name)
lakes
viruses
Injections
lake
fish
infection
RNA Viruses
RNA
pathogenesis

Keywords

  • detection
  • faeces
  • intragastric challenge
  • tilapia
  • tilapia lake virus

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Cite this

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title = "Intragastric and intracoelomic injection challenge models of tilapia lake virus infection in Nile tilapia (Oreochromis niloticus L.) fingerlings",
abstract = "To gain a better understanding of the pathogenesis of tilapia lake virus (TiLV) infections in Nile tilapia (Oreochromis niloticus), fingerlings were challenged with a single dose of 1 × 104 TCID50/fish of TiLV utilizing intracoelomic/intraperitoneal (ICch) or intragastric (IGch) routes. Acute mortalities were present in both groups, reaching 70 and 40{\%} in ICch and IGch after 10 days, respectively. Challenged fish presented erratic swimming, lethargy, anorexia, exophthalmia and cutaneous petechiae and ecchymoses. Histological changes in challenged groups included syncytial formation, intracytoplasmic inclusion bodies and multifocal hepatocellular degeneration and necrosis. In addition, multifocal areas of mild proliferation of glial cells and lymphocytic perivascular cuffing were observed in the brain of exposed challenged groups. TiLV RNA was detected in gills and faeces of challenged fish using quantitative reverse transcriptase–PCR, as well as in the tank water holding challenged fish. Moreover, TiLV RNA was detected in scrolls obtained from formalin-fixed paraffin-embedded tissue blocks from challenged fish. Results from this study suggest that IG methods represent an additional method to study the pathogenesis of the disease in this species, as it results in infection and diseases as in naturally occurring cases and does not bypass important mucosal immune responses as injectable routes do.",
keywords = "detection, faeces, intragastric challenge, tilapia, tilapia lake virus",
author = "Felipe Pierezan and Susan Yun and Win Surachetpong and {Soto Martinez}, Esteban",
year = "2019",
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T1 - Intragastric and intracoelomic injection challenge models of tilapia lake virus infection in Nile tilapia (Oreochromis niloticus L.) fingerlings

AU - Pierezan, Felipe

AU - Yun, Susan

AU - Surachetpong, Win

AU - Soto Martinez, Esteban

PY - 2019/1/1

Y1 - 2019/1/1

N2 - To gain a better understanding of the pathogenesis of tilapia lake virus (TiLV) infections in Nile tilapia (Oreochromis niloticus), fingerlings were challenged with a single dose of 1 × 104 TCID50/fish of TiLV utilizing intracoelomic/intraperitoneal (ICch) or intragastric (IGch) routes. Acute mortalities were present in both groups, reaching 70 and 40% in ICch and IGch after 10 days, respectively. Challenged fish presented erratic swimming, lethargy, anorexia, exophthalmia and cutaneous petechiae and ecchymoses. Histological changes in challenged groups included syncytial formation, intracytoplasmic inclusion bodies and multifocal hepatocellular degeneration and necrosis. In addition, multifocal areas of mild proliferation of glial cells and lymphocytic perivascular cuffing were observed in the brain of exposed challenged groups. TiLV RNA was detected in gills and faeces of challenged fish using quantitative reverse transcriptase–PCR, as well as in the tank water holding challenged fish. Moreover, TiLV RNA was detected in scrolls obtained from formalin-fixed paraffin-embedded tissue blocks from challenged fish. Results from this study suggest that IG methods represent an additional method to study the pathogenesis of the disease in this species, as it results in infection and diseases as in naturally occurring cases and does not bypass important mucosal immune responses as injectable routes do.

AB - To gain a better understanding of the pathogenesis of tilapia lake virus (TiLV) infections in Nile tilapia (Oreochromis niloticus), fingerlings were challenged with a single dose of 1 × 104 TCID50/fish of TiLV utilizing intracoelomic/intraperitoneal (ICch) or intragastric (IGch) routes. Acute mortalities were present in both groups, reaching 70 and 40% in ICch and IGch after 10 days, respectively. Challenged fish presented erratic swimming, lethargy, anorexia, exophthalmia and cutaneous petechiae and ecchymoses. Histological changes in challenged groups included syncytial formation, intracytoplasmic inclusion bodies and multifocal hepatocellular degeneration and necrosis. In addition, multifocal areas of mild proliferation of glial cells and lymphocytic perivascular cuffing were observed in the brain of exposed challenged groups. TiLV RNA was detected in gills and faeces of challenged fish using quantitative reverse transcriptase–PCR, as well as in the tank water holding challenged fish. Moreover, TiLV RNA was detected in scrolls obtained from formalin-fixed paraffin-embedded tissue blocks from challenged fish. Results from this study suggest that IG methods represent an additional method to study the pathogenesis of the disease in this species, as it results in infection and diseases as in naturally occurring cases and does not bypass important mucosal immune responses as injectable routes do.

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