Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments

Limin Hao, Melanie Thein, Ingrid Brust-Mascher, Gul Civelekoglu-Scholey, Yun Lu, Seyda Acar, Bram Prevo, Shai Shaham, Jonathan M. Scholey

Research output: Contribution to journalArticle

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Abstract

Sensory cilia are assembled and maintained by kinesin-2-dependent intraflagellar transport (IFT). We investigated whether two Caenorhabditis elegans Î ±- and β-tubulin isotypes, identified through mutants that lack their cilium distal segments, are delivered to their assembly sites by IFT. Mutations in conserved residues in both tubulins destabilize distal singlet microtubules. One isotype, TBB-4, assembles into microtubules at the tips of the axoneme core and distal segments, where the microtubule tip tracker EB1 is found, and localizes all along the cilium, whereas the other, TBA-5, concentrates in distal singlets. IFT assays, fluorescence recovery after photobleaching analysis and modelling indicate that the continual transport of sub-stoichiometric numbers of these tubulin subunits by the IFT machinery can maintain sensory cilia at their steady-state length.

Original languageEnglish (US)
Pages (from-to)790-798
Number of pages9
JournalNature Cell Biology
Volume13
Issue number7
DOIs
StatePublished - Jul 2011

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Cilia
Tubulin
Microtubules
Fluorescence Recovery After Photobleaching
Axoneme
Kinesin
Caenorhabditis elegans
Mutation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hao, L., Thein, M., Brust-Mascher, I., Civelekoglu-Scholey, G., Lu, Y., Acar, S., ... Scholey, J. M. (2011). Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments. Nature Cell Biology, 13(7), 790-798. https://doi.org/10.1038/ncb2268

Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments. / Hao, Limin; Thein, Melanie; Brust-Mascher, Ingrid; Civelekoglu-Scholey, Gul; Lu, Yun; Acar, Seyda; Prevo, Bram; Shaham, Shai; Scholey, Jonathan M.

In: Nature Cell Biology, Vol. 13, No. 7, 07.2011, p. 790-798.

Research output: Contribution to journalArticle

Hao, L, Thein, M, Brust-Mascher, I, Civelekoglu-Scholey, G, Lu, Y, Acar, S, Prevo, B, Shaham, S & Scholey, JM 2011, 'Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments', Nature Cell Biology, vol. 13, no. 7, pp. 790-798. https://doi.org/10.1038/ncb2268
Hao L, Thein M, Brust-Mascher I, Civelekoglu-Scholey G, Lu Y, Acar S et al. Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments. Nature Cell Biology. 2011 Jul;13(7):790-798. https://doi.org/10.1038/ncb2268
Hao, Limin ; Thein, Melanie ; Brust-Mascher, Ingrid ; Civelekoglu-Scholey, Gul ; Lu, Yun ; Acar, Seyda ; Prevo, Bram ; Shaham, Shai ; Scholey, Jonathan M. / Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments. In: Nature Cell Biology. 2011 ; Vol. 13, No. 7. pp. 790-798.
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