Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments

Limin Hao, Melanie Thein, Ingrid Brust-Mascher, Gul Civelekoglu-Scholey, Yun Lu, Seyda Acar, Bram Prevo, Shai Shaham, Jonathan M. Scholey

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

Sensory cilia are assembled and maintained by kinesin-2-dependent intraflagellar transport (IFT). We investigated whether two Caenorhabditis elegans Î ±- and β-tubulin isotypes, identified through mutants that lack their cilium distal segments, are delivered to their assembly sites by IFT. Mutations in conserved residues in both tubulins destabilize distal singlet microtubules. One isotype, TBB-4, assembles into microtubules at the tips of the axoneme core and distal segments, where the microtubule tip tracker EB1 is found, and localizes all along the cilium, whereas the other, TBA-5, concentrates in distal singlets. IFT assays, fluorescence recovery after photobleaching analysis and modelling indicate that the continual transport of sub-stoichiometric numbers of these tubulin subunits by the IFT machinery can maintain sensory cilia at their steady-state length.

Original languageEnglish (US)
Pages (from-to)790-798
Number of pages9
JournalNature Cell Biology
Volume13
Issue number7
DOIs
StatePublished - Jul 2011

ASJC Scopus subject areas

  • Cell Biology

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    Hao, L., Thein, M., Brust-Mascher, I., Civelekoglu-Scholey, G., Lu, Y., Acar, S., Prevo, B., Shaham, S., & Scholey, J. M. (2011). Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments. Nature Cell Biology, 13(7), 790-798. https://doi.org/10.1038/ncb2268