Abstract
Intraerythrocytic multiplication of Theileria parva in vitro: an ultrastructural study. International Journal for Parasitology 16: 223-229. Piroplasms of a Theileria parva isolate from Muguga, Kenya were maintained in short-term stationary erythrocyte cultures and the mode of multiplication studied by light and electron microscopy. Division of single piroplasms into four intraerythrocytic merozoites was observed within the first 24 h in vitro. After 8 days of cultivation, 10-20% of the parasitised erythrocytes contained quadruplet merozoite forms resulting from multiplication in vitro. Electronmicrographs showed that intraerythrocytic multiplication occurred by a schizogonous process that proceeded from the initial formation of rhoptries and electron-dense cytoplasmic plaques beneath the parasite's plasmalemmal membrane, to nuclear division and the final separation of a maximum of four differentiated merozoites. The merozoites formed by intra-erythrocytic schizogony in vitro had ultrastructural features identical to merozoites produced by intra-lymphocytic schizonts of T. parva.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 223-229 |
| Number of pages | 7 |
| Journal | International Journal for Parasitology |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1986 |
| Externally published | Yes |
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Keywords
- cultivation
- East Coast fever
- electron microscopy
- erythrocyte
- in vitro
- merozoites
- piroplasms
- schizogony
- Theileria parva
- ultrastructure
ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
Cite this
Intraerythrocytic multiplication of Theileria parva in vitro : An ultrastructural study. / Conrad, Patricia A; Denham, D.; Brown, C. G D.
In: International Journal for Parasitology, Vol. 16, No. 3, 1986, p. 223-229.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intraerythrocytic multiplication of Theileria parva in vitro
T2 - An ultrastructural study
AU - Conrad, Patricia A
AU - Denham, D.
AU - Brown, C. G D
PY - 1986
Y1 - 1986
N2 - Intraerythrocytic multiplication of Theileria parva in vitro: an ultrastructural study. International Journal for Parasitology 16: 223-229. Piroplasms of a Theileria parva isolate from Muguga, Kenya were maintained in short-term stationary erythrocyte cultures and the mode of multiplication studied by light and electron microscopy. Division of single piroplasms into four intraerythrocytic merozoites was observed within the first 24 h in vitro. After 8 days of cultivation, 10-20% of the parasitised erythrocytes contained quadruplet merozoite forms resulting from multiplication in vitro. Electronmicrographs showed that intraerythrocytic multiplication occurred by a schizogonous process that proceeded from the initial formation of rhoptries and electron-dense cytoplasmic plaques beneath the parasite's plasmalemmal membrane, to nuclear division and the final separation of a maximum of four differentiated merozoites. The merozoites formed by intra-erythrocytic schizogony in vitro had ultrastructural features identical to merozoites produced by intra-lymphocytic schizonts of T. parva.
AB - Intraerythrocytic multiplication of Theileria parva in vitro: an ultrastructural study. International Journal for Parasitology 16: 223-229. Piroplasms of a Theileria parva isolate from Muguga, Kenya were maintained in short-term stationary erythrocyte cultures and the mode of multiplication studied by light and electron microscopy. Division of single piroplasms into four intraerythrocytic merozoites was observed within the first 24 h in vitro. After 8 days of cultivation, 10-20% of the parasitised erythrocytes contained quadruplet merozoite forms resulting from multiplication in vitro. Electronmicrographs showed that intraerythrocytic multiplication occurred by a schizogonous process that proceeded from the initial formation of rhoptries and electron-dense cytoplasmic plaques beneath the parasite's plasmalemmal membrane, to nuclear division and the final separation of a maximum of four differentiated merozoites. The merozoites formed by intra-erythrocytic schizogony in vitro had ultrastructural features identical to merozoites produced by intra-lymphocytic schizonts of T. parva.
KW - cultivation
KW - East Coast fever
KW - electron microscopy
KW - erythrocyte
KW - in vitro
KW - merozoites
KW - piroplasms
KW - schizogony
KW - Theileria parva
KW - ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=0022732101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022732101&partnerID=8YFLogxK
U2 - 10.1016/0020-7519(86)90047-0
DO - 10.1016/0020-7519(86)90047-0
M3 - Article
C2 - 3091518
AN - SCOPUS:0022732101
VL - 16
SP - 223
EP - 229
JO - International Journal for Parasitology
JF - International Journal for Parasitology
SN - 0020-7519
IS - 3
ER -