Intracranial distribution of the sympathetic system in mice: DiI tracing and immunocytochemical labeling

Dominik R Haudenschild, John M. McPherson, Ross Tubo, Franois Binette

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The intracranial distribution of the cephalic brancnes of the superior cervical ganglion (scg) was studied in mice using indocarbocyanine dye (DiI) anterograde tracing. Two main branches were traced from the scg. The first branch joined the nerve of the pterygoid canal (the vidian nerve), npc, from which several intracranial sympathetic branches passed to the branches of the trigeminal nerve (tgn), abducent nerve (abn), trochlear nerve (trn), and oculomotor nerve (ocn). Most of the second branch joined the abn, from which sympathetic fibers dispersed in the distal region of the trigeminal ganglion (tgg) to forma plexus close to the ganglion's branches. Branches from this plexus joined the branches of the tgn, trn, and ocn. Several minor branches arising from the second branch of the scg were also observed. One formed a sympathetic plexus around the internal carotid artery (ica); a second formed a sympathetic plexus in the proximal region of tgg, close to its root; and a third branch coursed laterally to reach the ear by passing along the greater petrosal nerve (gpn). All of the intracranial trajectories traced from scg were found to be catecholaminergic, and likely sympathetic, using tyrosine hydroxylase (TH) immunocytochemistry.

Original languageEnglish (US)
Pages (from-to)99-111
Number of pages13
JournalAnatomical Record
Volume263
Issue number1
DOIs
StatePublished - May 1 2001
Externally publishedYes

Fingerprint

Superior Cervical Ganglion
nerve tissue
Trochlear Nerve
Abducens Nerve
Oculomotor Nerve
Trigeminal Ganglion
Trigeminal Nerve
mice
canal
dye
plexus
trajectory
Adrenergic Fibers
Tyrosine 3-Monooxygenase
Internal Carotid Artery
Ganglia
Ear
Coloring Agents
Immunohistochemistry
Head

Keywords

  • DiI tracing
  • Intracranial sympathetic nerves
  • Superior cervical ganglion
  • Tyrosine hydroxylase immunocytochemistry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Intracranial distribution of the sympathetic system in mice : DiI tracing and immunocytochemical labeling. / Haudenschild, Dominik R; McPherson, John M.; Tubo, Ross; Binette, Franois.

In: Anatomical Record, Vol. 263, No. 1, 01.05.2001, p. 99-111.

Research output: Contribution to journalArticle

Haudenschild, Dominik R ; McPherson, John M. ; Tubo, Ross ; Binette, Franois. / Intracranial distribution of the sympathetic system in mice : DiI tracing and immunocytochemical labeling. In: Anatomical Record. 2001 ; Vol. 263, No. 1. pp. 99-111.
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