Intracellular signalling mechanism responsible for modulation of sarcolemmal ATP-sensitive potassium channels by nitric oxide in ventricular cardiomyocytes

Dai Min Zhang, Yongping Chai, Jeffrey R. Erickson, Joan Heller Brown, Donald M Bers, Yu-Fung Lin

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Abstract

Key points: Both the ATP-sensitive potassium (KATP) channel and the gaseous messenger nitric oxide (NO) play fundamental roles in protecting the heart from injuries related to ischaemia. NO has previously been suggested to modulate cardiac KATP channels; however, the underlying mechanism remains largely unknown. In this study, by performing electrophysiological and biochemical assays, we demonstrate that NO potentiation of KATP channel activity in ventricular cardiomyocytes is prevented by pharmacological inhibition of soluble guanylyl cyclase (sGC), cGMP-dependent protein kinase (PKG), Ca2+/calmodulin-dependent protein kinase II (CaMKII) and extracellular signal-regulated protein kinase 1/2 (ERK1/2), by removal of reactive oxygen species and by genetic disruption of CaMKIIδ. These results suggest that NO modulates cardiac KATP channels via a novel cGMP-sGC-cGMP-PKG-ROS-ERK1/2-calmodulin-CaMKII (δ isoform in particular) signalling cascade. This novel intracellular signalling pathway may regulate the excitability of heart cells and provide protection against ischaemic or hypoxic injury, by opening the cardioprotective KATP channels. The ATP-sensitive potassium (KATP) channels are crucial for stress adaptation in the heart. It has previously been suggested that the function of KATP channels is modulated by nitric oxide (NO), a gaseous messenger known to be cytoprotective; however, the underlying mechanism remains poorly understood. Here we sought to delineate the intracellular signalling mechanism responsible for NO modulation of sarcolemmal KATP (sarcKATP) channels in ventricular cardiomyocytes. Cell-attached patch recordings were performed in transfected human embryonic kidney (HEK) 293 cells and ventricular cardiomyocytes freshly isolated from adult rabbits or genetically modified mice, in combination with pharmacological and biochemical approaches. Bath application of the NO donor NOC-18 increased the single-channel activity of Kir6.2/SUR2A (i.e. the principal ventricular-type KATP) channels in HEK293 cells, whereas the increase was abated by KT5823 [a selective cGMP-dependent protein kinase (PKG) inhibitor], mercaptopropionyl glycine [MPG; a reactive oxygen species (ROS) scavenger], catalase (an H2O2-degrading enzyme), myristoylated autocamtide-2 related inhibitory peptide (mAIP) selective for Ca2+/calmodulin-dependent protein kinase II (CaMKII) and U0126 [an extracellular signal-regulated protein kinase 1/2 (ERK1/2) inhibitor], respectively. The NO donors NOC-18 and N-(2-deoxy-α,β-d-glucopyranose-2-)-N2-acetyl-S-nitroso-d,l-penicillaminamide

Original languageEnglish (US)
Pages (from-to)971-990
Number of pages20
JournalJournal of Physiology
Volume592
Issue number5
DOIs
StatePublished - Mar 1 2014

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KATP Channels
Cardiac Myocytes
Nitric Oxide
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Mitogen-Activated Protein Kinase 3
Protein Kinases
Cyclic GMP-Dependent Protein Kinases
Reactive Oxygen Species
Nitric Oxide Donors
Pharmacology
Heart Injuries
Cytoprotection
HEK293 Cells
Calmodulin
Protein Kinase Inhibitors
Baths
Catalase
Glycine
Protein Isoforms
Ischemia

ASJC Scopus subject areas

  • Physiology

Cite this

Intracellular signalling mechanism responsible for modulation of sarcolemmal ATP-sensitive potassium channels by nitric oxide in ventricular cardiomyocytes. / Zhang, Dai Min; Chai, Yongping; Erickson, Jeffrey R.; Brown, Joan Heller; Bers, Donald M; Lin, Yu-Fung.

In: Journal of Physiology, Vol. 592, No. 5, 01.03.2014, p. 971-990.

Research output: Contribution to journalArticle

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