Intracellular proteins of feline immunodeficiency virus and their antigenic relationship with equine infectious anaemia virus proteins

H. F. Egberink, J. Ederveen, R. C. Montelaro, Niels C Pedersen, M. C. Horzinek, M. J M Koolen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Feline immunodeficiency virus (FIV) grown in cat lymphocyte and thymocyte cultures was labelled with L-[35S]methionine or [3H]glucosamine and virus-coded proteins were identified using immunoprecipitation. Polypeptides with apparent M(r) values of 15K, 24K, 43K, 50K, 120K and 160K were detected. An additional polypeptide of 10K was detected by Western blot analysis. The two highest M(r) species sometimes appeared as one band, of which only the 120K polypeptide was glycosylated. In the presence of tunicamycin gp 120 was no longer detectable and a non-glycosylated precursor of 75K was found instead. Pulse-chase experiments suggested that the smaller polypeptides p24 and p15 are cleavage products of both p160 and p50. Western blot analysis using a rabbit serum directed against p26 of equine infectious anaemia virus (EIAV) and an anti-EIAV horse serum from a field case of infection revealed a cross-reactivity with p24 of FIV. Cat sera collected late after experimental FIV infection recognized p26 of EIAV, indicating a reciprocal cross-reactivity.

Original languageEnglish (US)
Pages (from-to)739-743
Number of pages5
JournalJournal of General Virology
Volume71
Issue number3
StatePublished - 1990
Externally publishedYes

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Equine infectious anemia virus
Feline Immunodeficiency Virus
Peptides
Proteins
Cats
Western Blotting
Serum
Tunicamycin
Glucosamine
Virus Diseases
Thymocytes
Immunoprecipitation
Methionine
Horses
Lymphocytes
Rabbits
Viruses
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Intracellular proteins of feline immunodeficiency virus and their antigenic relationship with equine infectious anaemia virus proteins. / Egberink, H. F.; Ederveen, J.; Montelaro, R. C.; Pedersen, Niels C; Horzinek, M. C.; Koolen, M. J M.

In: Journal of General Virology, Vol. 71, No. 3, 1990, p. 739-743.

Research output: Contribution to journalArticle

Egberink, H. F. ; Ederveen, J. ; Montelaro, R. C. ; Pedersen, Niels C ; Horzinek, M. C. ; Koolen, M. J M. / Intracellular proteins of feline immunodeficiency virus and their antigenic relationship with equine infectious anaemia virus proteins. In: Journal of General Virology. 1990 ; Vol. 71, No. 3. pp. 739-743.
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AU - Ederveen, J.

AU - Montelaro, R. C.

AU - Pedersen, Niels C

AU - Horzinek, M. C.

AU - Koolen, M. J M

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