Abstract
α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors that lack the glutamate receptor GluR2 subunit are Ca2+-permeable and exhibit inwardly rectifying current responses to kainate and AMPA. A proportion of cultured rat hippocampal neurons show similar Ca2+-permeable inwardly rectifying AMPA receptor currents. Inward rectification in these neurons was lost with intracellular dialysis and was not present in excised outside-out patches but was maintained in perforated-patch whole-cell recordings, suggesting that a diffusible cytoplasmic factor may be responsible for rectification. Inclusion of the naturally occurring polyamines spermine and spermidine in the recording pipette prevented loss of rectification in both whole-cell and excisedpatch recordings; Mg2+ and putrescine were without effect. Inward rectification of Ca2+-permeable AMPA receptors may reflect voltage-dependent channel block by intracellular polyamines.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9298-9302 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 92 |
| Issue number | 20 |
| State | Published - Sep 26 1995 |
| Externally published | Yes |
Fingerprint
Keywords
- Excitatory aniino acid receptor
- Spermidine
- Spermine
ASJC Scopus subject areas
- General
- Genetics
Cite this
Intracellular polyamines mediate inward rectification of Ca2+-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors. / Donevan, Sean D.; Rogawski, Michael A.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 20, 26.09.1995, p. 9298-9302.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intracellular polyamines mediate inward rectification of Ca2+-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors
AU - Donevan, Sean D.
AU - Rogawski, Michael A
PY - 1995/9/26
Y1 - 1995/9/26
N2 - α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors that lack the glutamate receptor GluR2 subunit are Ca2+-permeable and exhibit inwardly rectifying current responses to kainate and AMPA. A proportion of cultured rat hippocampal neurons show similar Ca2+-permeable inwardly rectifying AMPA receptor currents. Inward rectification in these neurons was lost with intracellular dialysis and was not present in excised outside-out patches but was maintained in perforated-patch whole-cell recordings, suggesting that a diffusible cytoplasmic factor may be responsible for rectification. Inclusion of the naturally occurring polyamines spermine and spermidine in the recording pipette prevented loss of rectification in both whole-cell and excisedpatch recordings; Mg2+ and putrescine were without effect. Inward rectification of Ca2+-permeable AMPA receptors may reflect voltage-dependent channel block by intracellular polyamines.
AB - α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors that lack the glutamate receptor GluR2 subunit are Ca2+-permeable and exhibit inwardly rectifying current responses to kainate and AMPA. A proportion of cultured rat hippocampal neurons show similar Ca2+-permeable inwardly rectifying AMPA receptor currents. Inward rectification in these neurons was lost with intracellular dialysis and was not present in excised outside-out patches but was maintained in perforated-patch whole-cell recordings, suggesting that a diffusible cytoplasmic factor may be responsible for rectification. Inclusion of the naturally occurring polyamines spermine and spermidine in the recording pipette prevented loss of rectification in both whole-cell and excisedpatch recordings; Mg2+ and putrescine were without effect. Inward rectification of Ca2+-permeable AMPA receptors may reflect voltage-dependent channel block by intracellular polyamines.
KW - Excitatory aniino acid receptor
KW - Spermidine
KW - Spermine
UR - http://www.scopus.com/inward/record.url?scp=0029056120&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029056120&partnerID=8YFLogxK
M3 - Article
C2 - 7568121
AN - SCOPUS:0029056120
VL - 92
SP - 9298
EP - 9302
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 20
ER -