Intracellular pH sensors based on surface-enhanced raman scattering

Chad E. Talley; Leonard Jusinski; Christopher W. Hollars; Stephen M. Lane; Thomas R Huser

doi:10.1021/ac049093j

Intracellular pH sensors based on surface-enhanced raman scattering

Chad E. Talley, Leonard Jusinski, Christopher W. Hollars, Stephen M. Lane, Thomas R Huser

Endocrinology, Diabetes, and Metabolism

Research output: Contribution to journal › Article

262 Scopus citations

Abstract

We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

Original language	English (US)
Pages (from-to)	7064-7068
Number of pages	5
Journal	Analytical Chemistry
Volume	76
Issue number	23
DOIs	https://doi.org/10.1021/ac049093j
State	Published - Jan 3 2005

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ASJC Scopus subject areas

Analytical Chemistry

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Talley, C. E., Jusinski, L., Hollars, C. W., Lane, S. M., & Huser, T. R. (2005). Intracellular pH sensors based on surface-enhanced raman scattering. Analytical Chemistry, 76(23), 7064-7068. https://doi.org/10.1021/ac049093j

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AB - We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

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10.1021/ac049093j