Intracellular pH sensors based on surface-enhanced raman scattering

Chad E. Talley; Leonard Jusinski; Christopher W. Hollars; Stephen M. Lane; Thomas R Huser

doi:10.1021/ac049093j

Intracellular pH sensors based on surface-enhanced raman scattering

Chad E. Talley, Leonard Jusinski, Christopher W. Hollars, Stephen M. Lane, Thomas R Huser

Endocrinology, Diabetes, and Metabolism

Research output: Contribution to journal › Article › peer-review

278 Scopus citations

Abstract

We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

Original language	English (US)
Pages (from-to)	7064-7068
Number of pages	5
Journal	Analytical Chemistry
Volume	76
Issue number	23
DOIs	https://doi.org/10.1021/ac049093j
State	Published - Jan 3 2005

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac049093j

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abstract = "We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.",

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AU - Talley, Chad E.

AU - Jusinski, Leonard

AU - Hollars, Christopher W.

AU - Lane, Stephen M.

AU - Huser, Thomas R

PY - 2005/1/3

Y1 - 2005/1/3

N2 - We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

AB - We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

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Intracellular pH sensors based on surface-enhanced raman scattering

Abstract

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