Intracellular pH sensors based on surface-enhanced raman scattering

Chad E. Talley; Leonard Jusinski; Christopher W. Hollars; Stephen M. Lane; Thomas R Huser

doi:10.1021/ac049093j

Intracellular pH sensors based on surface-enhanced raman scattering

Chad E. Talley, Leonard Jusinski, Christopher W. Hollars, Stephen M. Lane, Thomas R Huser

Endocrinology, Diabetes, and Metabolism

Research output: Contribution to journal › Article › peer-review

278 Scopus citations

Abstract

We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

Original language	English (US)
Pages (from-to)	7064-7068
Number of pages	5
Journal	Analytical Chemistry
Volume	76
Issue number	23
DOIs	https://doi.org/10.1021/ac049093j
State	Published - Jan 3 2005

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.1021/ac049093j

Fingerprint Dive into the research topics of 'Intracellular pH sensors based on surface-enhanced raman scattering'. Together they form a unique fingerprint.

Cite this

@article{846928ae03864902a43c1d39691b837c,

title = "Intracellular pH sensors based on surface-enhanced raman scattering",

abstract = "We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.",

author = "Talley, {Chad E.} and Leonard Jusinski and Hollars, {Christopher W.} and Lane, {Stephen M.} and Huser, {Thomas R}",

year = "2005",

month = jan,

day = "3",

doi = "10.1021/ac049093j",

language = "English (US)",

volume = "76",

pages = "7064--7068",

journal = "Industrial And Engineering Chemistry Analytical Edition",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "23",

}

TY - JOUR

T1 - Intracellular pH sensors based on surface-enhanced raman scattering

AU - Talley, Chad E.

AU - Jusinski, Leonard

AU - Hollars, Christopher W.

AU - Lane, Stephen M.

AU - Huser, Thomas R

PY - 2005/1/3

Y1 - 2005/1/3

N2 - We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

AB - We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

UR - http://www.scopus.com/inward/record.url?scp=10044246283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10044246283&partnerID=8YFLogxK

U2 - 10.1021/ac049093j

DO - 10.1021/ac049093j

M3 - Article

C2 - 15571360

AN - SCOPUS:10044246283

VL - 76

SP - 7064

EP - 7068

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 23

ER -

Intracellular pH sensors based on surface-enhanced raman scattering

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this