Intracellular pH sensors based on surface-enhanced raman scattering

Chad E. Talley, Leonard Jusinski, Christopher W. Hollars, Stephen M. Lane, Thomas R Huser

Research output: Contribution to journalArticlepeer-review

293 Scopus citations


We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

Original languageEnglish (US)
Pages (from-to)7064-7068
Number of pages5
JournalAnalytical Chemistry
Issue number23
StatePublished - Jan 3 2005

ASJC Scopus subject areas

  • Analytical Chemistry


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