Abstract
We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7064-7068 |
| Number of pages | 5 |
| Journal | Analytical Chemistry |
| Volume | 76 |
| Issue number | 23 |
| DOIs | |
| State | Published - Jan 3 2005 |
ASJC Scopus subject areas
- Analytical Chemistry
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Cite this
Talley, C. E., Jusinski, L., Hollars, C. W., Lane, S. M., & Huser, T. R. (2005). Intracellular pH sensors based on surface-enhanced raman scattering. Analytical Chemistry, 76(23), 7064-7068. https://doi.org/10.1021/ac049093j