Intracellular forms of adenovirus DNA: integrated form of adenovirus DNA appears early in productive infection

J. Schick, K. Baczko, E. Fanning, J. Groneberg, Harold Burger, W. Doerfler

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Abstract

In KB cells productively infected with adenovirus type 2, alkali stable >100S and 40-100S viral DNAs are synthesized starting 2-4 hr postinfection, i.e., before unit length (34 S) viral DNA is made. The amount of >100S and 40-100S viral DNA increases when 34S viral DNA synthesis begins, and at 16-18 hr postinfection, the 40-100S viral DNA represents 5-20% of the total intracellular viral DNA. The 40-100S viral DNA is synthesized throughout infection. Part of the 40-100S DNA synthesized 5-8 hr postinfection has a density in alkaline CsCl gradients intermediate between those of viral and cellular DNAs. This finding indicates that newly synthesized viral DNA is covalently linked to cellular DNA. Viral sequences can be excised from the cellular DNA of infected cells with the EcoRI restriction endonuclease. Fragments of viral DNA are detected in polyacrylamide agarose gels by DNA DNA hybridization, and these fragments correspond in size to most of the known EcoRI fragments of adenovirus 2 DNA. Viral DNA sequences in size classes between the EcoRI and C fragments are also found and probably represent viral DNA linked to cellular sequences.

Original languageEnglish (US)
Pages (from-to)1043-1047
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number4
DOIs
StatePublished - Dec 1 1976
Externally publishedYes

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