Intracellular dyes mask immunoreactivity of hippocampal interneurons

H. E. Scharfman, D. D. Kunkel, Philip A Schwartzkroin

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


The results of several studies have suggested that local circuit neurons, or interneurons, of area CA1 of hippocampus use γ-aminobutyric acid (GABA) as their neurotransmitter. However, when these cells were labelled by intracellular dye injection, and examined immunocytochemically with antisera raised against GABA, none of the interneurons were immunoreactive. Numerous non-injected interneurons in the same tissue section were clearly immunoreactive. These results suggest that intracellular dyes interfere with immunocytochemical staining of hippocampal interneurons.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalNeuroscience Letters
Issue number1
StatePublished - Jan 2 1989
Externally publishedYes


  • Horseradish peroxidase
  • Immunocytochemistry
  • Interneuron
  • Lucifer yellow
  • Paryalbumin
  • Pyramidal cell
  • Somatostatin
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Neuroscience(all)


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