Intracellular cytokine production by fetal and adult monocytes

André Hebra, Phillip Strange, J. Michael Egbert, Mohamed R Ali, Ashley Mullinax, Edward Buchanan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background/Purpose: In light of the neonate's increased susceptibility to systemic infection, the authors hypothesized that adult and fetal monocytes have different cytokine expression profiles in response to lipopolysaccharide (LPS), and interleukin (IL)-11, a counter-inflammatory cytokine. Methods: Samples of cord blood (n = 30) and adult blood (n = 30) were obtained and treated as follows: control (baseline expression), LPS exposure, and IL-11 or IL-11+LPS exposure. After incubation with a protein transport inhibitor, mononuclear cells were stained for intracellular tumor necrosis factor (TNF)-α, IL-1β, IL-6, and IL-8. Each sample was then analyzed by flow cytometry for cytokine expression. Cytokine production was measured by the percent positive as well as the fluorescence index for each cytokine. Analysis of variance (ANOVA) and Students t tests were used for statistical analysis. Results: Baseline levels of IL-8 were significantly higher for fetal monocytes (P < .0001). After LPS exposure, fetal monocytes produced less TNF-α (P = .0105) and more IL-8 (P < .0007) relative to adult cells. IL-11 treatment reduced baseline production of IL-8 in fetal and adult monocytes (P < .05). Conclusions: These results suggest that neonatal monocytes portray a different cytokine expression profile compared with adult monocytes. IL-11 treatment appears to alter the IL-8 expression of resting fetal and adult monocytes.

Original languageEnglish (US)
Pages (from-to)1321-1326
Number of pages6
JournalJournal of Pediatric Surgery
Volume36
Issue number9
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Interleukin-11
Monocytes
Interleukin-8
Cytokines
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Protein Transport
Fetal Blood
Interleukin-1
Interleukin-6
Analysis of Variance
Flow Cytometry
Fluorescence
Newborn Infant
Students
Therapeutics
Infection

Keywords

  • Cytokines
  • Fetal
  • Interleukin-11
  • Monocytes

ASJC Scopus subject areas

  • Surgery

Cite this

Hebra, A., Strange, P., Egbert, J. M., Ali, M. R., Mullinax, A., & Buchanan, E. (2001). Intracellular cytokine production by fetal and adult monocytes. Journal of Pediatric Surgery, 36(9), 1321-1326. https://doi.org/10.1053/jpsu.2001.26359

Intracellular cytokine production by fetal and adult monocytes. / Hebra, André; Strange, Phillip; Egbert, J. Michael; Ali, Mohamed R; Mullinax, Ashley; Buchanan, Edward.

In: Journal of Pediatric Surgery, Vol. 36, No. 9, 2001, p. 1321-1326.

Research output: Contribution to journalArticle

Hebra, A, Strange, P, Egbert, JM, Ali, MR, Mullinax, A & Buchanan, E 2001, 'Intracellular cytokine production by fetal and adult monocytes', Journal of Pediatric Surgery, vol. 36, no. 9, pp. 1321-1326. https://doi.org/10.1053/jpsu.2001.26359
Hebra, André ; Strange, Phillip ; Egbert, J. Michael ; Ali, Mohamed R ; Mullinax, Ashley ; Buchanan, Edward. / Intracellular cytokine production by fetal and adult monocytes. In: Journal of Pediatric Surgery. 2001 ; Vol. 36, No. 9. pp. 1321-1326.
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