Intracellular cytokine expression in the CD4+ and CD8+ T cells from intestinal mucosa of simian immunodeficiency virus infected macaques

Zeljka McBride, Joseph J. Mattapallil, Francois Villinger, Aftab A. Ansari, Satya Dandekar

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Isolated intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) from jejunum of SIV infected animals were examined for alterations in basal cytokine expression by RT-PCR. Remarkable changes in IFNy and EL-10 RNA levels were observed in IEL and LPL in SIV infection while IL-4 and IL-2 RNA levels remained unaltered. In addition, the CD4+ and CD8+ LPL were examined for intracellular cytokine production following mitogenic activation by flowcytometry. Both CD4+ and CD8+ T lymphocytes in intestinal mucosa retained the potential to produce IFNγ in response to mitogenic stimulation in vitro, without a remarkable change in IL-4 production. The dominant IFNγ cytokine response could be one of the major contributing factors in SIV associated enteropathy.

Original languageEnglish (US)
Pages (from-to)129-140
Number of pages12
JournalJournal of Medical Primatology
Volume27
Issue number2-3
StatePublished - 1998

Keywords

  • AIDS
  • Enteropathy
  • HIV
  • IEL
  • IFNγ
  • IL-10
  • Intracellular flow cytometry
  • LPL
  • Nonhuman primates
  • SIV

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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