Intracellular cytokine expression in the CD4+ and CD8+ T cells from intestinal mucosa of simian immunodeficiency virus infected macaques

Zeljka McBride, Joseph J. Mattapallil, Francois Villinger, Aftab A. Ansari, Satya Dandekar

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Isolated intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) from jejunum of SIV infected animals were examined for alterations in basal cytokine expression by RT-PCR. Remarkable changes in IFNy and EL-10 RNA levels were observed in IEL and LPL in SIV infection while IL-4 and IL-2 RNA levels remained unaltered. In addition, the CD4+ and CD8+ LPL were examined for intracellular cytokine production following mitogenic activation by flowcytometry. Both CD4+ and CD8+ T lymphocytes in intestinal mucosa retained the potential to produce IFNγ in response to mitogenic stimulation in vitro, without a remarkable change in IL-4 production. The dominant IFNγ cytokine response could be one of the major contributing factors in SIV associated enteropathy.

Original languageEnglish (US)
Pages (from-to)129-140
Number of pages12
JournalJournal of Medical Primatology
Volume27
Issue number2-3
StatePublished - 1998

Keywords

  • AIDS
  • Enteropathy
  • HIV
  • IEL
  • IFNγ
  • IL-10
  • Intracellular flow cytometry
  • LPL
  • Nonhuman primates
  • SIV

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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