Intracellular cytokine and chemokine production by peripheral T cell subsets from HIV-infected individuals following antiretroviral therapy

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Abstract

The purpose of this study was to measure expression of IFN-γ, IL-2, IL-4, IL-10 and MIP-1β in CD4+ , CD8+ , γδ1 + and γδ2 + T lymphocytes in peripheral blood and to examine how these responses are affected by the antiretroviral regimen. Two HIV-1 infected patients were given combination therapy (AZT, 3TC and saquinavir). Plasma HIV-1 RNA (quantitative PCR) and CD4+ and CD8+ T cell numbers were monitored. Intracellular chemokine and cytokine production was studied in CD4 + , CD8+ αβ T lymphocytes and γδ T lymphocytes in whole blood samples by flow cytometry. A decline in the viral load was observed at 4 weeks post-treatment. HIV-infected patients had elevated numbers of IFN-γ producing CD4+ and CD8+ T cells prior to treatment as compared to uninfected controls. These cells had increased potential of IFN-γ production that correlated with higher viral load. There was no difference in the number of IL-4 producing cells in patients compared to the controls or during treatment. The percentage of MIP-1β producing cells were higher for both CD4+ and CD8+ T cells in HIV-infected patients compared to the controls. These levels remained high during therapy, MIP-1β expression was detected in T cells although they failed to produce IFN-γ and IL-2 with mitogenic stimulation. The γδ T cells were major producers of both IFN-γ and MIP-1β. Our results indicate that an evaluation of cytokine/chemokine expression in T cells in HIV-1 infected patients could be valuable in assessing the restoration of host immune function.

Original languageEnglish (US)
Pages (from-to)393
Number of pages1
JournalClinical Infectious Diseases
Volume25
Issue number2
StatePublished - 1997

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T-Lymphocyte Subsets
Chemokines
HIV
Cytokines
T-Lymphocytes
HIV-1
Therapeutics
Viral Load
Interleukin-4
Interleukin-2
Saquinavir
Lamivudine
Interleukin-10
Flow Cytometry
Cell Count
RNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

Cite this

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abstract = "The purpose of this study was to measure expression of IFN-γ, IL-2, IL-4, IL-10 and MIP-1β in CD4+ , CD8+ , γδ1 + and γδ2 + T lymphocytes in peripheral blood and to examine how these responses are affected by the antiretroviral regimen. Two HIV-1 infected patients were given combination therapy (AZT, 3TC and saquinavir). Plasma HIV-1 RNA (quantitative PCR) and CD4+ and CD8+ T cell numbers were monitored. Intracellular chemokine and cytokine production was studied in CD4 + , CD8+ αβ T lymphocytes and γδ T lymphocytes in whole blood samples by flow cytometry. A decline in the viral load was observed at 4 weeks post-treatment. HIV-infected patients had elevated numbers of IFN-γ producing CD4+ and CD8+ T cells prior to treatment as compared to uninfected controls. These cells had increased potential of IFN-γ production that correlated with higher viral load. There was no difference in the number of IL-4 producing cells in patients compared to the controls or during treatment. The percentage of MIP-1β producing cells were higher for both CD4+ and CD8+ T cells in HIV-infected patients compared to the controls. These levels remained high during therapy, MIP-1β expression was detected in T cells although they failed to produce IFN-γ and IL-2 with mitogenic stimulation. The γδ T cells were major producers of both IFN-γ and MIP-1β. Our results indicate that an evaluation of cytokine/chemokine expression in T cells in HIV-1 infected patients could be valuable in assessing the restoration of host immune function.",
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