Intracellular calcium oscillations in cell populations of ras- transfected I-7 subline of human HaAaT keratinocytes

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Abstract

We have observed oscillations of intracellular Ca2+ (Ca(i)) concentration in populations of ras-transfected HaCaT keratinocytes of I-7 subline. In postconfluent monolayers of I-7 keratinocytes, an increase in extracellular Ca2+ (Ca(o)) concentration to 0.25-0.5 mM induced sinusoidal Ca(i) oscillations, which persisted longer than 1 h with amplitudes of 50- 150 nM and periods of 5-10 min. Thapsigargin, which depletes internal Ca2+ stores, did not prevent Cat-induced Ca(i) oscillations, and it also induced Ca(i) oscillations in the ras-transfected I-7 line. Removal of extracellular Ca2+ or addition of Ca2+-entry blocker La3+ or SKandF 96365 inhibited Ca(i) oscillations, suggesting that Ca(i) oscillations in ras-transfected HaCaT keratinocytes were dependent on Ca2+ influx across the plasma membrane. Because the Ca(o)-induced Ca(i) oscillations have been observed only in ras-transfected I-7 subline and not in its nontransfected parental HaCaT line, this may provide a partial explanation for the divergent responses of ras-transfected and nontransfected keratinocytes to Ca(o) signal for control of growth and differentiation.

Original languageEnglish (US)
Pages (from-to)765-769
Number of pages5
JournalJournal of Investigative Dermatology
Volume109
Issue number6
StatePublished - 1997

Keywords

  • Ca(i) homeostasis
  • Ca signal
  • Cell growth and differentiation
  • Thapsigargin

ASJC Scopus subject areas

  • Dermatology

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