Intracellular calcium and sodium activity in sheep heart Purkinje fibres - Effect of changes of external sodium and intracellular pH

Donald M Bers, David Ellis

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112 Citations (Scopus)

Abstract

Intracellular Ca, Na and H selective microelectrodes were used to study the effects of reduction of the extracellular Na concentration, [Na]0, on the free intracellular Ca concentration, [Ca]i, Na activity, (aNa i), and intracellular pH (pHi) in sheep heart Purkinje fibres. 1. Reduction of [Na]0 from 140 mM to 14 mM produced a decrease of aNa i, and increase of free [Ca]i, and normally an increase of resting tension. 2. Inhibition of the Na-K pump by 10-5 M acetylstrophanthidin produced a slow rise of [Ca]i and resting tension. 3. The magnitude of the increase of free [Ca]i (and tension) produced by [Na]0 reduction was greatly enhanced when the Na-K pump is inhibited by either acetylstrophanthidin or K-free solutions. 4. We suggest that this enhanced rise of free [Ca]i in the presence of Na-K pump inhibition is due to Ca loading of intracellular Ca buffering systems during the pump inhibition. 5. Addition of NH4Cl produced a transient decrease of free [Ca]i that accompanied an alkaline change in pHi. Removal of NH4Cl (which produced a transient intracellular acidification) produced a transient increase of free [Ca]i. We conclude that a close relationship exists between the control of free [Ca]i and pHi which may be due to competition at, or common use of, intracellular buffering systems.

Original languageEnglish (US)
Pages (from-to)171-178
Number of pages8
JournalPflügers Archiv European Journal of Physiology
Volume393
Issue number2
DOIs
StatePublished - Apr 1982
Externally publishedYes

Fingerprint

Purkinje Fibers
Sheep
Sodium
Pumps
Calcium
Fibers
Microelectrodes
Acidification
acetylstrophanthidin

Keywords

  • Calcium
  • Cardiac glycoside
  • Heart
  • pH
  • Purkinje fibres
  • Sodium

ASJC Scopus subject areas

  • Physiology

Cite this

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abstract = "Intracellular Ca, Na and H selective microelectrodes were used to study the effects of reduction of the extracellular Na concentration, [Na]0, on the free intracellular Ca concentration, [Ca]i, Na activity, (aNa i), and intracellular pH (pHi) in sheep heart Purkinje fibres. 1. Reduction of [Na]0 from 140 mM to 14 mM produced a decrease of aNa i, and increase of free [Ca]i, and normally an increase of resting tension. 2. Inhibition of the Na-K pump by 10-5 M acetylstrophanthidin produced a slow rise of [Ca]i and resting tension. 3. The magnitude of the increase of free [Ca]i (and tension) produced by [Na]0 reduction was greatly enhanced when the Na-K pump is inhibited by either acetylstrophanthidin or K-free solutions. 4. We suggest that this enhanced rise of free [Ca]i in the presence of Na-K pump inhibition is due to Ca loading of intracellular Ca buffering systems during the pump inhibition. 5. Addition of NH4Cl produced a transient decrease of free [Ca]i that accompanied an alkaline change in pHi. Removal of NH4Cl (which produced a transient intracellular acidification) produced a transient increase of free [Ca]i. We conclude that a close relationship exists between the control of free [Ca]i and pHi which may be due to competition at, or common use of, intracellular buffering systems.",
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AU - Ellis, David

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