Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques

Augusto F. Schmidt, Paranthaman S. Kannan, Claire A. Chougnet, Steve C. Danzer, Lisa Miller, Alan H. Jobe, Suhas G. Kallapur

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Chorioamnionitis is associated with an increased risk of brain injury in preterm neonates. Inflammatory changes in brain could underlie this injury. Here, we evaluated whether neuroinflammation is induced by chorioamnionitis in a clinically relevant model. Methods: Rhesus macaque fetuses were exposed to either intra-amniotic (IA) saline, or IA lipopolysaccharide (LPS) (1 mg) 16 or 48 h prior to delivery at 130 days (85 % of gestation) (n = 4-5 animals/group). We measured cytokines in the cerebrospinal fluid (CSF), froze samples from the left brain for molecular analysis, and immersion fixed the right brain hemisphere for immunohistology. We analyzed the messenger RNA (mRNA) levels of the pro-inflammatory cytokines IL-1β, CCL2, TNF-aα, IL-6, IL-8, IL-10, and COX-2 in the periventricular white matter (PVWM), cortex, thalamus, hippocampus, and cerebellum by RT-qPCR. Brain injury was assessed by immunohistology for myelin basic protein (MBP), IBA1 (microglial marker), GFAP (astrocyte marker), OLIG2 (oligodendrocyte marker), NeuN (neuronal marker), CD3 (T cells), and CD14 (monocytes). Microglial proliferation was assessed by co-immunostaining for IBA1 and Ki67. Data were analyzed by ANOVA with Tukey's post-test. Results: IA LPS increased mRNA expression of pro-inflammatory cytokines in the PVWM, thalamus, and cerebellum, increased IL-6 concentration in the CSF, and increased apoptosis in the periventricular area after 16 h. Microglial proliferation in the white matter was increased 48 h after IA LPS. Conclusions: LPS-induced chorioamnionitis caused neuroinflammation, microglial proliferation, and periventricular apoptosis in a clinically relevant model of chorioamnionitis in fetal rhesus macaques. These findings identify specific responses in the fetal brain and support the hypothesis that neuroinflammatory changes may mediate the adverse neurodevelopmental outcomes associated with chorioamnionitis.

Original languageEnglish (US)
Article number238
JournalJournal of Neuroinflammation
Volume13
Issue number1
DOIs
StatePublished - Sep 6 2016

Fingerprint

Chorioamnionitis
Macaca mulatta
Lipopolysaccharides
Brain
Cytokines
Thalamus
Brain Injuries
Cerebellum
Cerebrospinal Fluid
Interleukin-6
Apoptosis
Messenger RNA
Myelin Basic Protein
Oligodendroglia
Immersion
Interleukin-8
Interleukin-1
Astrocytes
Interleukin-10
Monocytes

Keywords

  • Apoptosis
  • Brain injury
  • Chorioamnionitis
  • Cytokines
  • Microglia
  • Periventricular leukomalacia
  • Prematurity

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Schmidt, A. F., Kannan, P. S., Chougnet, C. A., Danzer, S. C., Miller, L., Jobe, A. H., & Kallapur, S. G. (2016). Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. Journal of Neuroinflammation, 13(1), [238]. https://doi.org/10.1186/s12974-016-0706-4

Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. / Schmidt, Augusto F.; Kannan, Paranthaman S.; Chougnet, Claire A.; Danzer, Steve C.; Miller, Lisa; Jobe, Alan H.; Kallapur, Suhas G.

In: Journal of Neuroinflammation, Vol. 13, No. 1, 238, 06.09.2016.

Research output: Contribution to journalArticle

Schmidt, AF, Kannan, PS, Chougnet, CA, Danzer, SC, Miller, L, Jobe, AH & Kallapur, SG 2016, 'Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques', Journal of Neuroinflammation, vol. 13, no. 1, 238. https://doi.org/10.1186/s12974-016-0706-4
Schmidt, Augusto F. ; Kannan, Paranthaman S. ; Chougnet, Claire A. ; Danzer, Steve C. ; Miller, Lisa ; Jobe, Alan H. ; Kallapur, Suhas G. / Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. In: Journal of Neuroinflammation. 2016 ; Vol. 13, No. 1.
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AU - Kallapur, Suhas G.

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N2 - Background: Chorioamnionitis is associated with an increased risk of brain injury in preterm neonates. Inflammatory changes in brain could underlie this injury. Here, we evaluated whether neuroinflammation is induced by chorioamnionitis in a clinically relevant model. Methods: Rhesus macaque fetuses were exposed to either intra-amniotic (IA) saline, or IA lipopolysaccharide (LPS) (1 mg) 16 or 48 h prior to delivery at 130 days (85 % of gestation) (n = 4-5 animals/group). We measured cytokines in the cerebrospinal fluid (CSF), froze samples from the left brain for molecular analysis, and immersion fixed the right brain hemisphere for immunohistology. We analyzed the messenger RNA (mRNA) levels of the pro-inflammatory cytokines IL-1β, CCL2, TNF-aα, IL-6, IL-8, IL-10, and COX-2 in the periventricular white matter (PVWM), cortex, thalamus, hippocampus, and cerebellum by RT-qPCR. Brain injury was assessed by immunohistology for myelin basic protein (MBP), IBA1 (microglial marker), GFAP (astrocyte marker), OLIG2 (oligodendrocyte marker), NeuN (neuronal marker), CD3 (T cells), and CD14 (monocytes). Microglial proliferation was assessed by co-immunostaining for IBA1 and Ki67. Data were analyzed by ANOVA with Tukey's post-test. Results: IA LPS increased mRNA expression of pro-inflammatory cytokines in the PVWM, thalamus, and cerebellum, increased IL-6 concentration in the CSF, and increased apoptosis in the periventricular area after 16 h. Microglial proliferation in the white matter was increased 48 h after IA LPS. Conclusions: LPS-induced chorioamnionitis caused neuroinflammation, microglial proliferation, and periventricular apoptosis in a clinically relevant model of chorioamnionitis in fetal rhesus macaques. These findings identify specific responses in the fetal brain and support the hypothesis that neuroinflammatory changes may mediate the adverse neurodevelopmental outcomes associated with chorioamnionitis.

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