Abstract
Analysis of the X-ray crystallographic structure of an 8-methoxypsoralen-thymine monoadduct has led to the suggestion that psoralen cross-links would bend DNA by as much as 70° [Peckler, S., Graves, B., Kanne, D., Rapoport, H., Hearst, J. E., & Kim, S.-H. (1982) J. Mol. Biol. 162, 157-172]. DNA can exist in a stably bent configuration in solution as recently demonstrated from analysis of polyacrylamide gel electrophoresis and differential decay of birefringence. Using these techniques, we have investigated the structure of DNA cross-linked with 8-methoxypsoralen and 4,5′,8-trimethylpsoralen. The results are not consistent with cross-links introducing any appreciable stable bend in double-stranded DNA molecules. Results suggest that photobound 4,5′,8-trimethylpsoralen molecules lengthen DNA by the equivalent of about one base pair per photobound adduct. We have also determined that 4,5′,8-trimethylpsoralen cross-links are introduced preferentially into 5′-TA compared to 5′-AT DNA sequences.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6299-6303 |
| Number of pages | 5 |
| Journal | Biochemistry |
| Volume | 23 |
| Issue number | 26 |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Interstrand psoralen cross-links do not introduce appreciable bends in DNA. / Sinden, Richard R.; Hagerman, Paul J.
In: Biochemistry, Vol. 23, No. 26, 1984, p. 6299-6303.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interstrand psoralen cross-links do not introduce appreciable bends in DNA
AU - Sinden, Richard R.
AU - Hagerman, Paul J
PY - 1984
Y1 - 1984
N2 - Analysis of the X-ray crystallographic structure of an 8-methoxypsoralen-thymine monoadduct has led to the suggestion that psoralen cross-links would bend DNA by as much as 70° [Peckler, S., Graves, B., Kanne, D., Rapoport, H., Hearst, J. E., & Kim, S.-H. (1982) J. Mol. Biol. 162, 157-172]. DNA can exist in a stably bent configuration in solution as recently demonstrated from analysis of polyacrylamide gel electrophoresis and differential decay of birefringence. Using these techniques, we have investigated the structure of DNA cross-linked with 8-methoxypsoralen and 4,5′,8-trimethylpsoralen. The results are not consistent with cross-links introducing any appreciable stable bend in double-stranded DNA molecules. Results suggest that photobound 4,5′,8-trimethylpsoralen molecules lengthen DNA by the equivalent of about one base pair per photobound adduct. We have also determined that 4,5′,8-trimethylpsoralen cross-links are introduced preferentially into 5′-TA compared to 5′-AT DNA sequences.
AB - Analysis of the X-ray crystallographic structure of an 8-methoxypsoralen-thymine monoadduct has led to the suggestion that psoralen cross-links would bend DNA by as much as 70° [Peckler, S., Graves, B., Kanne, D., Rapoport, H., Hearst, J. E., & Kim, S.-H. (1982) J. Mol. Biol. 162, 157-172]. DNA can exist in a stably bent configuration in solution as recently demonstrated from analysis of polyacrylamide gel electrophoresis and differential decay of birefringence. Using these techniques, we have investigated the structure of DNA cross-linked with 8-methoxypsoralen and 4,5′,8-trimethylpsoralen. The results are not consistent with cross-links introducing any appreciable stable bend in double-stranded DNA molecules. Results suggest that photobound 4,5′,8-trimethylpsoralen molecules lengthen DNA by the equivalent of about one base pair per photobound adduct. We have also determined that 4,5′,8-trimethylpsoralen cross-links are introduced preferentially into 5′-TA compared to 5′-AT DNA sequences.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021709188&partnerID=8YFLogxK
M3 - Article
C2 - 6529552
AN - SCOPUS:0021709188
VL - 23
SP - 6299
EP - 6303
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 26
ER -