Interstitial exclusion of IgG in rat tissues estimated by continuous infusion

H. Wiig, George Kaysen, H. A. Al-Bander, M. De Carlo, L. Sibley, E. M. Renkin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Interstitial exclusion, defined as the fraction of interstitial fluid volume inaccessible to a solute, was evaluated for immunoglobulin G (IgG) in selected tissues of rats by a method previously applied to serum albumin (29). IgG distribution volumes were also measured for intestine. 125I- labeled rat IgG was infused for 5 or 7 days (n = 4 rats each) with an implanted osmotic pump (Alzet). At the termination of infusion, the rat was anesthetized, nephrectomized, and injected with 51Cr-labeled EDTA (4 h) to label total extracellular fluid volume and 131I-labeled bovine IgG (5 min) to label plasma volume. Samples of skin, muscle, and tendon were assayed for total and extractable tracer activity. Interstitial fluid from these tissues was sampled postmortem with nylon wicks for assay of 125I-labeled IgG and endogenous albumin and IgG. Exclusion of IgG was calculated from the difference between extravascular 125I-labeled IgG and 51Cr-labeled EDTA distribution volumes. In contrast to our previous experience with tracer albumin, 125I-labeled IgG was not fully extractable from minced skin, muscle, or tendon by isotonic saline; only 71-83% was recovered under conditions that eluted 92-96% of tracer albumin and 94-99% of tracer EDTA. We conclude that ~20% of extravascular 125I-labeled IgG in these tissues is sequestered or bound in the interstitium. Calculation of IgG fractional exclusion from extractable tracer yielded the following values (means ± SE, n = 8 rats): leg muscles 0.37 ± 0.09, leg skin 0.44 ± 0.03, back skin 0.36 ± 0.04, tail skin 0.40 ± 0.08, and tail tendon 0.55 ± 0.04. None of these values is significantly different from the corresponding albumin exclusion fractions. It seems possible that the steric factors, which might be expected to increase IgG exclusion relative to albumin, are balanced by electrostatic differences, which would increase albumin exclusion relative to IgG.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume266
Issue number1 35-1
StatePublished - 1994

Fingerprint

immunoglobulin G
Immunoglobulin G
rats
albumins
Albumins
skin (animal)
tracer techniques
extracellular fluids
Extracellular Fluid
Skin
tendons
Edetic Acid
Tendons
Muscles
tissues
muscles
Tail
Leg
legs
tail

Keywords

  • bound immunoglobulin G
  • creatine-ethylenediaminetetraacetic acid space
  • immunoglobulin G space
  • interstitial fluid immunoglobulin G concentration
  • intestine
  • muscle
  • skin
  • tendon
  • total tissue immunoglobulin G
  • total water content

ASJC Scopus subject areas

  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Interstitial exclusion of IgG in rat tissues estimated by continuous infusion. / Wiig, H.; Kaysen, George; Al-Bander, H. A.; De Carlo, M.; Sibley, L.; Renkin, E. M.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 266, No. 1 35-1, 1994.

Research output: Contribution to journalArticle

Wiig, H. ; Kaysen, George ; Al-Bander, H. A. ; De Carlo, M. ; Sibley, L. ; Renkin, E. M. / Interstitial exclusion of IgG in rat tissues estimated by continuous infusion. In: American Journal of Physiology - Heart and Circulatory Physiology. 1994 ; Vol. 266, No. 1 35-1.
@article{c2c998308e6045059cf9b229997ee744,
title = "Interstitial exclusion of IgG in rat tissues estimated by continuous infusion",
abstract = "Interstitial exclusion, defined as the fraction of interstitial fluid volume inaccessible to a solute, was evaluated for immunoglobulin G (IgG) in selected tissues of rats by a method previously applied to serum albumin (29). IgG distribution volumes were also measured for intestine. 125I- labeled rat IgG was infused for 5 or 7 days (n = 4 rats each) with an implanted osmotic pump (Alzet). At the termination of infusion, the rat was anesthetized, nephrectomized, and injected with 51Cr-labeled EDTA (4 h) to label total extracellular fluid volume and 131I-labeled bovine IgG (5 min) to label plasma volume. Samples of skin, muscle, and tendon were assayed for total and extractable tracer activity. Interstitial fluid from these tissues was sampled postmortem with nylon wicks for assay of 125I-labeled IgG and endogenous albumin and IgG. Exclusion of IgG was calculated from the difference between extravascular 125I-labeled IgG and 51Cr-labeled EDTA distribution volumes. In contrast to our previous experience with tracer albumin, 125I-labeled IgG was not fully extractable from minced skin, muscle, or tendon by isotonic saline; only 71-83{\%} was recovered under conditions that eluted 92-96{\%} of tracer albumin and 94-99{\%} of tracer EDTA. We conclude that ~20{\%} of extravascular 125I-labeled IgG in these tissues is sequestered or bound in the interstitium. Calculation of IgG fractional exclusion from extractable tracer yielded the following values (means ± SE, n = 8 rats): leg muscles 0.37 ± 0.09, leg skin 0.44 ± 0.03, back skin 0.36 ± 0.04, tail skin 0.40 ± 0.08, and tail tendon 0.55 ± 0.04. None of these values is significantly different from the corresponding albumin exclusion fractions. It seems possible that the steric factors, which might be expected to increase IgG exclusion relative to albumin, are balanced by electrostatic differences, which would increase albumin exclusion relative to IgG.",
keywords = "bound immunoglobulin G, creatine-ethylenediaminetetraacetic acid space, immunoglobulin G space, interstitial fluid immunoglobulin G concentration, intestine, muscle, skin, tendon, total tissue immunoglobulin G, total water content",
author = "H. Wiig and George Kaysen and Al-Bander, {H. A.} and {De Carlo}, M. and L. Sibley and Renkin, {E. M.}",
year = "1994",
language = "English (US)",
volume = "266",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "1 35-1",

}

TY - JOUR

T1 - Interstitial exclusion of IgG in rat tissues estimated by continuous infusion

AU - Wiig, H.

AU - Kaysen, George

AU - Al-Bander, H. A.

AU - De Carlo, M.

AU - Sibley, L.

AU - Renkin, E. M.

PY - 1994

Y1 - 1994

N2 - Interstitial exclusion, defined as the fraction of interstitial fluid volume inaccessible to a solute, was evaluated for immunoglobulin G (IgG) in selected tissues of rats by a method previously applied to serum albumin (29). IgG distribution volumes were also measured for intestine. 125I- labeled rat IgG was infused for 5 or 7 days (n = 4 rats each) with an implanted osmotic pump (Alzet). At the termination of infusion, the rat was anesthetized, nephrectomized, and injected with 51Cr-labeled EDTA (4 h) to label total extracellular fluid volume and 131I-labeled bovine IgG (5 min) to label plasma volume. Samples of skin, muscle, and tendon were assayed for total and extractable tracer activity. Interstitial fluid from these tissues was sampled postmortem with nylon wicks for assay of 125I-labeled IgG and endogenous albumin and IgG. Exclusion of IgG was calculated from the difference between extravascular 125I-labeled IgG and 51Cr-labeled EDTA distribution volumes. In contrast to our previous experience with tracer albumin, 125I-labeled IgG was not fully extractable from minced skin, muscle, or tendon by isotonic saline; only 71-83% was recovered under conditions that eluted 92-96% of tracer albumin and 94-99% of tracer EDTA. We conclude that ~20% of extravascular 125I-labeled IgG in these tissues is sequestered or bound in the interstitium. Calculation of IgG fractional exclusion from extractable tracer yielded the following values (means ± SE, n = 8 rats): leg muscles 0.37 ± 0.09, leg skin 0.44 ± 0.03, back skin 0.36 ± 0.04, tail skin 0.40 ± 0.08, and tail tendon 0.55 ± 0.04. None of these values is significantly different from the corresponding albumin exclusion fractions. It seems possible that the steric factors, which might be expected to increase IgG exclusion relative to albumin, are balanced by electrostatic differences, which would increase albumin exclusion relative to IgG.

AB - Interstitial exclusion, defined as the fraction of interstitial fluid volume inaccessible to a solute, was evaluated for immunoglobulin G (IgG) in selected tissues of rats by a method previously applied to serum albumin (29). IgG distribution volumes were also measured for intestine. 125I- labeled rat IgG was infused for 5 or 7 days (n = 4 rats each) with an implanted osmotic pump (Alzet). At the termination of infusion, the rat was anesthetized, nephrectomized, and injected with 51Cr-labeled EDTA (4 h) to label total extracellular fluid volume and 131I-labeled bovine IgG (5 min) to label plasma volume. Samples of skin, muscle, and tendon were assayed for total and extractable tracer activity. Interstitial fluid from these tissues was sampled postmortem with nylon wicks for assay of 125I-labeled IgG and endogenous albumin and IgG. Exclusion of IgG was calculated from the difference between extravascular 125I-labeled IgG and 51Cr-labeled EDTA distribution volumes. In contrast to our previous experience with tracer albumin, 125I-labeled IgG was not fully extractable from minced skin, muscle, or tendon by isotonic saline; only 71-83% was recovered under conditions that eluted 92-96% of tracer albumin and 94-99% of tracer EDTA. We conclude that ~20% of extravascular 125I-labeled IgG in these tissues is sequestered or bound in the interstitium. Calculation of IgG fractional exclusion from extractable tracer yielded the following values (means ± SE, n = 8 rats): leg muscles 0.37 ± 0.09, leg skin 0.44 ± 0.03, back skin 0.36 ± 0.04, tail skin 0.40 ± 0.08, and tail tendon 0.55 ± 0.04. None of these values is significantly different from the corresponding albumin exclusion fractions. It seems possible that the steric factors, which might be expected to increase IgG exclusion relative to albumin, are balanced by electrostatic differences, which would increase albumin exclusion relative to IgG.

KW - bound immunoglobulin G

KW - creatine-ethylenediaminetetraacetic acid space

KW - immunoglobulin G space

KW - interstitial fluid immunoglobulin G concentration

KW - intestine

KW - muscle

KW - skin

KW - tendon

KW - total tissue immunoglobulin G

KW - total water content

UR - http://www.scopus.com/inward/record.url?scp=0028121553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028121553&partnerID=8YFLogxK

M3 - Article

C2 - 8304502

AN - SCOPUS:0028121553

VL - 266

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 1 35-1

ER -