Abstract
Interstitial exclusion, defined as the fraction of interstitial fluid volume inaccessible to a solute, was evaluated for immunoglobulin G (IgG) in selected tissues of rats by a method previously applied to serum albumin (29). IgG distribution volumes were also measured for intestine. 125I- labeled rat IgG was infused for 5 or 7 days (n = 4 rats each) with an implanted osmotic pump (Alzet). At the termination of infusion, the rat was anesthetized, nephrectomized, and injected with 51Cr-labeled EDTA (4 h) to label total extracellular fluid volume and 131I-labeled bovine IgG (5 min) to label plasma volume. Samples of skin, muscle, and tendon were assayed for total and extractable tracer activity. Interstitial fluid from these tissues was sampled postmortem with nylon wicks for assay of 125I-labeled IgG and endogenous albumin and IgG. Exclusion of IgG was calculated from the difference between extravascular 125I-labeled IgG and 51Cr-labeled EDTA distribution volumes. In contrast to our previous experience with tracer albumin, 125I-labeled IgG was not fully extractable from minced skin, muscle, or tendon by isotonic saline; only 71-83% was recovered under conditions that eluted 92-96% of tracer albumin and 94-99% of tracer EDTA. We conclude that ~20% of extravascular 125I-labeled IgG in these tissues is sequestered or bound in the interstitium. Calculation of IgG fractional exclusion from extractable tracer yielded the following values (means ± SE, n = 8 rats): leg muscles 0.37 ± 0.09, leg skin 0.44 ± 0.03, back skin 0.36 ± 0.04, tail skin 0.40 ± 0.08, and tail tendon 0.55 ± 0.04. None of these values is significantly different from the corresponding albumin exclusion fractions. It seems possible that the steric factors, which might be expected to increase IgG exclusion relative to albumin, are balanced by electrostatic differences, which would increase albumin exclusion relative to IgG.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Heart and Circulatory Physiology |
| Volume | 266 |
| Issue number | 1 35-1 |
| State | Published - 1994 |
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Keywords
- bound immunoglobulin G
- creatine-ethylenediaminetetraacetic acid space
- immunoglobulin G space
- interstitial fluid immunoglobulin G concentration
- intestine
- muscle
- skin
- tendon
- total tissue immunoglobulin G
- total water content
ASJC Scopus subject areas
- Physiology
- Agricultural and Biological Sciences(all)
Cite this
Interstitial exclusion of IgG in rat tissues estimated by continuous infusion. / Wiig, H.; Kaysen, George; Al-Bander, H. A.; De Carlo, M.; Sibley, L.; Renkin, E. M.
In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 266, No. 1 35-1, 1994.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interstitial exclusion of IgG in rat tissues estimated by continuous infusion
AU - Wiig, H.
AU - Kaysen, George
AU - Al-Bander, H. A.
AU - De Carlo, M.
AU - Sibley, L.
AU - Renkin, E. M.
PY - 1994
Y1 - 1994
N2 - Interstitial exclusion, defined as the fraction of interstitial fluid volume inaccessible to a solute, was evaluated for immunoglobulin G (IgG) in selected tissues of rats by a method previously applied to serum albumin (29). IgG distribution volumes were also measured for intestine. 125I- labeled rat IgG was infused for 5 or 7 days (n = 4 rats each) with an implanted osmotic pump (Alzet). At the termination of infusion, the rat was anesthetized, nephrectomized, and injected with 51Cr-labeled EDTA (4 h) to label total extracellular fluid volume and 131I-labeled bovine IgG (5 min) to label plasma volume. Samples of skin, muscle, and tendon were assayed for total and extractable tracer activity. Interstitial fluid from these tissues was sampled postmortem with nylon wicks for assay of 125I-labeled IgG and endogenous albumin and IgG. Exclusion of IgG was calculated from the difference between extravascular 125I-labeled IgG and 51Cr-labeled EDTA distribution volumes. In contrast to our previous experience with tracer albumin, 125I-labeled IgG was not fully extractable from minced skin, muscle, or tendon by isotonic saline; only 71-83% was recovered under conditions that eluted 92-96% of tracer albumin and 94-99% of tracer EDTA. We conclude that ~20% of extravascular 125I-labeled IgG in these tissues is sequestered or bound in the interstitium. Calculation of IgG fractional exclusion from extractable tracer yielded the following values (means ± SE, n = 8 rats): leg muscles 0.37 ± 0.09, leg skin 0.44 ± 0.03, back skin 0.36 ± 0.04, tail skin 0.40 ± 0.08, and tail tendon 0.55 ± 0.04. None of these values is significantly different from the corresponding albumin exclusion fractions. It seems possible that the steric factors, which might be expected to increase IgG exclusion relative to albumin, are balanced by electrostatic differences, which would increase albumin exclusion relative to IgG.
AB - Interstitial exclusion, defined as the fraction of interstitial fluid volume inaccessible to a solute, was evaluated for immunoglobulin G (IgG) in selected tissues of rats by a method previously applied to serum albumin (29). IgG distribution volumes were also measured for intestine. 125I- labeled rat IgG was infused for 5 or 7 days (n = 4 rats each) with an implanted osmotic pump (Alzet). At the termination of infusion, the rat was anesthetized, nephrectomized, and injected with 51Cr-labeled EDTA (4 h) to label total extracellular fluid volume and 131I-labeled bovine IgG (5 min) to label plasma volume. Samples of skin, muscle, and tendon were assayed for total and extractable tracer activity. Interstitial fluid from these tissues was sampled postmortem with nylon wicks for assay of 125I-labeled IgG and endogenous albumin and IgG. Exclusion of IgG was calculated from the difference between extravascular 125I-labeled IgG and 51Cr-labeled EDTA distribution volumes. In contrast to our previous experience with tracer albumin, 125I-labeled IgG was not fully extractable from minced skin, muscle, or tendon by isotonic saline; only 71-83% was recovered under conditions that eluted 92-96% of tracer albumin and 94-99% of tracer EDTA. We conclude that ~20% of extravascular 125I-labeled IgG in these tissues is sequestered or bound in the interstitium. Calculation of IgG fractional exclusion from extractable tracer yielded the following values (means ± SE, n = 8 rats): leg muscles 0.37 ± 0.09, leg skin 0.44 ± 0.03, back skin 0.36 ± 0.04, tail skin 0.40 ± 0.08, and tail tendon 0.55 ± 0.04. None of these values is significantly different from the corresponding albumin exclusion fractions. It seems possible that the steric factors, which might be expected to increase IgG exclusion relative to albumin, are balanced by electrostatic differences, which would increase albumin exclusion relative to IgG.
KW - bound immunoglobulin G
KW - creatine-ethylenediaminetetraacetic acid space
KW - immunoglobulin G space
KW - interstitial fluid immunoglobulin G concentration
KW - intestine
KW - muscle
KW - skin
KW - tendon
KW - total tissue immunoglobulin G
KW - total water content
UR - http://www.scopus.com/inward/record.url?scp=0028121553&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028121553&partnerID=8YFLogxK
M3 - Article
C2 - 8304502
AN - SCOPUS:0028121553
VL - 266
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 1 35-1
ER -