Interrogation of N-linked oligosaccharides using infrared multiphoton dissociation in FT-ICR Mass Spectrometry

Katherine S. Lancaster, Hyun Joo An, Bensheng Li, Carlito B Lebrilla

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The structural elucidation of oligosaccharides remains a major challenge. Mass spectrometry provides a rapid and convenient method for structural elucidation based on tandem mass spectrometry. Ions commonly are selected and subjected to collision-induced dissociation (CID) to obtain structural information. Unfortunately, N-linked oligosaccharides are relatively large compounds and are not readily fragmented using CID. In this report, we illustrate the use of infrared multiphoton dissociation (IRMPD) to obtain structural information for large N-linked oligosaccharides. The IRMPD and CID behavior of oligosaccharides were compared for high-mannose-type oligosaccharides. Fragmentation that could not be obtained through conventional CID in Fourier transform ion cyclotron resonance mass spectrometry was observed with N-linked oligosaccharides. O-Linked and N-linked glycans of similarly large sizes were compared. It was found that internal cross-ring cleavages were observed only for N-linked oligosaccharides. The mannose branch points of N-linked oligosaccharides are apparently more susceptible to cross-ring cleavages.

Original languageEnglish (US)
Pages (from-to)4990-4997
Number of pages8
JournalAnalytical Chemistry
Volume78
Issue number14
DOIs
StatePublished - Jul 15 2006

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Oligosaccharides
Mass spectrometry
Infrared radiation
Mannose
Ions
Cyclotron resonance
Polysaccharides
Fourier transforms

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Interrogation of N-linked oligosaccharides using infrared multiphoton dissociation in FT-ICR Mass Spectrometry. / Lancaster, Katherine S.; An, Hyun Joo; Li, Bensheng; Lebrilla, Carlito B.

In: Analytical Chemistry, Vol. 78, No. 14, 15.07.2006, p. 4990-4997.

Research output: Contribution to journalArticle

Lancaster, Katherine S. ; An, Hyun Joo ; Li, Bensheng ; Lebrilla, Carlito B. / Interrogation of N-linked oligosaccharides using infrared multiphoton dissociation in FT-ICR Mass Spectrometry. In: Analytical Chemistry. 2006 ; Vol. 78, No. 14. pp. 4990-4997.
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