Interplay between DNA sequence and negative superhelicity drives R-loop structures

Robert Stolz, Shaheen Sulthana, Stella R. Hartono, Maika Malig, Craig J. Benham, Frederic Chedin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

R-loops are abundant three-stranded nucleic-acid structures that form in cis during transcription. Experimental evidence suggests that R-loop formation is affected by DNA sequence and topology. However, the exact manner by which these factors interact to determine R-loop susceptibility is unclear. To investigate this, we developed a statistical mechanical equilibrium model of R-loop formation in superhelical DNA. In this model, the energy involved in forming an R-loop includes four terms-junctional and basepairing energies and energies associated with superhelicity and with the torsional winding of the displaced DNA single strand around the RNA:DNA hybrid. This model shows that the significant energy barrier imposed by the formation of junctions can be overcome in two ways. First, base-pairing energy can favor RNA:DNA over DNA:DNA duplexes in favorable sequences. Second, R-loops, by absorbing negative superhelicity, partially or fully relax the rest of the DNA domain, thereby returning it to a lower energy state. In vitro transcription assays confirmed that R-loops cause plasmid relaxation and that negative superhelicity is required for R-loops to form, even in a favorable region. Single-molecule R-loop footprinting following in vitro transcription showed a strong agreement between theoretical predictions and experimental mapping of stable R-loop positions and further revealed the impact of DNA topology on the R-loop distribution landscape. Our results clarify the interplay between base sequence and DNA superhelicity in controlling R-loop stability. They also reveal R-loops as powerful and reversible topology sinks that cells may use to nonenzymatically relieve superhelical stress during transcription.

Original languageEnglish (US)
Pages (from-to)6260-6269
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number13
DOIs
StatePublished - Jan 1 2019

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DNA
RNA
Superhelical DNA
Base Pairing
Nucleic Acids
Plasmids
In Vitro Techniques

Keywords

  • DNA topology
  • Modeling
  • R-loop
  • Transcription

ASJC Scopus subject areas

  • General

Cite this

Interplay between DNA sequence and negative superhelicity drives R-loop structures. / Stolz, Robert; Sulthana, Shaheen; Hartono, Stella R.; Malig, Maika; Benham, Craig J.; Chedin, Frederic.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 13, 01.01.2019, p. 6260-6269.

Research output: Contribution to journalArticle

Stolz, Robert ; Sulthana, Shaheen ; Hartono, Stella R. ; Malig, Maika ; Benham, Craig J. ; Chedin, Frederic. / Interplay between DNA sequence and negative superhelicity drives R-loop structures. In: Proceedings of the National Academy of Sciences of the United States of America. 2019 ; Vol. 116, No. 13. pp. 6260-6269.
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