Abstract
RyR1 is an intracellular calcium channel with a central role in muscle contraction. We obtained a three-dimensional reconstruction of the RyR1 in the closed state at a nominal resolution of ∼10 Å using cryo-EM. The cytoplasmic assembly consists of a series of interconnected tubular structures that merge into four columns that extend into the transmembrane assembly. The transmembrane assembly, which has at least six transmembrane α-helices per monomer, has four tilted rods that can be fitted with the inner helices of a closed K+ channel atomic structure. The rods splay out at the lumenal side and converge into a dense ring at the cytoplasmic side. Another set of four rods emerges from this ring and shapes the inner part of the four columns. The resulting constricted axial structure provides direct continuity between cytoplasmic and transmembrane assemblies, and a possible mechanism for control of channel gating through conformational changes in the cytoplasmic assembly.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 539-544 |
| Number of pages | 6 |
| Journal | Nature Structural and Molecular Biology |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Structural Biology
- Molecular Biology
Cite this
Internal structure and visualization of transmembrane domains of the RyR1 calcium release channel by cryo-EM. / Samsó, Montserrat; Wagenknecht, Terence; Allen, P. D.
In: Nature Structural and Molecular Biology, Vol. 12, No. 6, 06.2005, p. 539-544.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Internal structure and visualization of transmembrane domains of the RyR1 calcium release channel by cryo-EM
AU - Samsó, Montserrat
AU - Wagenknecht, Terence
AU - Allen, P. D.
PY - 2005/6
Y1 - 2005/6
N2 - RyR1 is an intracellular calcium channel with a central role in muscle contraction. We obtained a three-dimensional reconstruction of the RyR1 in the closed state at a nominal resolution of ∼10 Å using cryo-EM. The cytoplasmic assembly consists of a series of interconnected tubular structures that merge into four columns that extend into the transmembrane assembly. The transmembrane assembly, which has at least six transmembrane α-helices per monomer, has four tilted rods that can be fitted with the inner helices of a closed K+ channel atomic structure. The rods splay out at the lumenal side and converge into a dense ring at the cytoplasmic side. Another set of four rods emerges from this ring and shapes the inner part of the four columns. The resulting constricted axial structure provides direct continuity between cytoplasmic and transmembrane assemblies, and a possible mechanism for control of channel gating through conformational changes in the cytoplasmic assembly.
AB - RyR1 is an intracellular calcium channel with a central role in muscle contraction. We obtained a three-dimensional reconstruction of the RyR1 in the closed state at a nominal resolution of ∼10 Å using cryo-EM. The cytoplasmic assembly consists of a series of interconnected tubular structures that merge into four columns that extend into the transmembrane assembly. The transmembrane assembly, which has at least six transmembrane α-helices per monomer, has four tilted rods that can be fitted with the inner helices of a closed K+ channel atomic structure. The rods splay out at the lumenal side and converge into a dense ring at the cytoplasmic side. Another set of four rods emerges from this ring and shapes the inner part of the four columns. The resulting constricted axial structure provides direct continuity between cytoplasmic and transmembrane assemblies, and a possible mechanism for control of channel gating through conformational changes in the cytoplasmic assembly.
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U2 - 10.1038/nsmb938
DO - 10.1038/nsmb938
M3 - Article
VL - 12
SP - 539
EP - 544
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
SN - 1545-9993
IS - 6
ER -