Interleukin 9 promotes influx and local maturation of eosinophils

J. Louahed, Y. Zhou, W. Lee Maloy, P. U. Rani, C. Weiss, Y. Tomer, A. Vink, J. C. Renauld, J. Van Snick, N. C. Nicolaides, R. C. Levitt, Angela Franciska Haczku

Research output: Contribution to journalArticle

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Abstract

The interleukin 9 (IL-9) pathway has recently been associated with the asthmatic phenotype including an eosinophilic tissue inflammation. The mechanism by which IL-9 affects eosinophils (eos) is not known. To investigate whether this cytokine has a direct activity on the development of eos and eosinophilic inflammation, a model of thioglycolate-induced peritoneal inflammation was used in IL-9 transgenic (TG5) and background strain (FVB) mice. In this model, a transient eosinophilic infiltration in the peritoneal cavity was observed in FVB mice 12 to 24 hours after thioglycolate injection that coincided with peak IL-5 and IL-9 release. In contrast, TG5 mice developed a massive eosinophilia that persisted at high levels (81% of total cells) even 72 hours after thioglycolate injection. Release of eosinophilic major basic protein (MBP), IL-4, and IL-5 to the peritoneal cavity of these mice was significantly increased when compared with the control FVB strain. To study the mechanism by which IL-9 exerts its effect on eos, bone marrow or peritoneal cells were cultured in the presence of IL-5, IL-9, or their combination in vitro. IL-5 alone was able to generate significant numbers of eos in TG5 but not FVB mice, whereas a combination of IL-5 and IL-9 induced marked eosinophilia in both strains indicating a synergism between these 2 cytokines. These data suggest that IL-9 may promote and sustain eosinophilic inflammation via IL-5-driven eos maturation of precursors.

Original languageEnglish (US)
Pages (from-to)1035-1042
Number of pages8
JournalBlood
Volume97
Issue number4
DOIs
StatePublished - Feb 15 2001
Externally publishedYes

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Interleukin-9
Eosinophils
Interleukin-5
Thioglycolates
Inflammation
Peritoneal Cavity
Eosinophilia
Strain control
Cytokines
Infiltration
Injections
Interleukin-4
Bone
Tissue
Cultured Cells
Bone Marrow

ASJC Scopus subject areas

  • Hematology

Cite this

Louahed, J., Zhou, Y., Lee Maloy, W., Rani, P. U., Weiss, C., Tomer, Y., ... Haczku, A. F. (2001). Interleukin 9 promotes influx and local maturation of eosinophils. Blood, 97(4), 1035-1042. https://doi.org/10.1182/blood.V97.4.1035

Interleukin 9 promotes influx and local maturation of eosinophils. / Louahed, J.; Zhou, Y.; Lee Maloy, W.; Rani, P. U.; Weiss, C.; Tomer, Y.; Vink, A.; Renauld, J. C.; Van Snick, J.; Nicolaides, N. C.; Levitt, R. C.; Haczku, Angela Franciska.

In: Blood, Vol. 97, No. 4, 15.02.2001, p. 1035-1042.

Research output: Contribution to journalArticle

Louahed, J, Zhou, Y, Lee Maloy, W, Rani, PU, Weiss, C, Tomer, Y, Vink, A, Renauld, JC, Van Snick, J, Nicolaides, NC, Levitt, RC & Haczku, AF 2001, 'Interleukin 9 promotes influx and local maturation of eosinophils', Blood, vol. 97, no. 4, pp. 1035-1042. https://doi.org/10.1182/blood.V97.4.1035
Louahed J, Zhou Y, Lee Maloy W, Rani PU, Weiss C, Tomer Y et al. Interleukin 9 promotes influx and local maturation of eosinophils. Blood. 2001 Feb 15;97(4):1035-1042. https://doi.org/10.1182/blood.V97.4.1035
Louahed, J. ; Zhou, Y. ; Lee Maloy, W. ; Rani, P. U. ; Weiss, C. ; Tomer, Y. ; Vink, A. ; Renauld, J. C. ; Van Snick, J. ; Nicolaides, N. C. ; Levitt, R. C. ; Haczku, Angela Franciska. / Interleukin 9 promotes influx and local maturation of eosinophils. In: Blood. 2001 ; Vol. 97, No. 4. pp. 1035-1042.
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abstract = "The interleukin 9 (IL-9) pathway has recently been associated with the asthmatic phenotype including an eosinophilic tissue inflammation. The mechanism by which IL-9 affects eosinophils (eos) is not known. To investigate whether this cytokine has a direct activity on the development of eos and eosinophilic inflammation, a model of thioglycolate-induced peritoneal inflammation was used in IL-9 transgenic (TG5) and background strain (FVB) mice. In this model, a transient eosinophilic infiltration in the peritoneal cavity was observed in FVB mice 12 to 24 hours after thioglycolate injection that coincided with peak IL-5 and IL-9 release. In contrast, TG5 mice developed a massive eosinophilia that persisted at high levels (81{\%} of total cells) even 72 hours after thioglycolate injection. Release of eosinophilic major basic protein (MBP), IL-4, and IL-5 to the peritoneal cavity of these mice was significantly increased when compared with the control FVB strain. To study the mechanism by which IL-9 exerts its effect on eos, bone marrow or peritoneal cells were cultured in the presence of IL-5, IL-9, or their combination in vitro. IL-5 alone was able to generate significant numbers of eos in TG5 but not FVB mice, whereas a combination of IL-5 and IL-9 induced marked eosinophilia in both strains indicating a synergism between these 2 cytokines. These data suggest that IL-9 may promote and sustain eosinophilic inflammation via IL-5-driven eos maturation of precursors.",
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