Interleukin-5 expression in the lung epithelium of transgenic mice leads to pulmonary changes pathognomonic of asthma

James J. Lee, Michael P. McGarry, Steven C. Farmer, Karen L. Denzler, Kirsten A. Larson, Patricia E. Carrigan, Ina E. Brenneise, Margaret A. Horton, Angela Franciska Haczku, Erwin W. Gelfand, George D. Leikauf, Nancy A. Lee

Research output: Contribution to journalArticle

439 Citations (Scopus)

Abstract

We have generated transgenic mice that constitutively express murine interleukin (IL)-5 in the lung epithelium. Airway expression of this cytokine resulted in a dramatic accumulation of peribronchial eosinophils and striking pathologic changes including the expansion of bronchus associated lymphoid tissue (BALT), goblet cell hyperplasia, epithelial hypertrophy, and focal collagen deposition. These changes were also accompanied by eosinophil infiltration of the airway lumen. In addition, transgenic animals displayed airway hyperresponsiveness to methacholine in the absence of aerosolized antigen challenge. These findings demonstrate that lung-specific IL-5 expression can induce pathologic changes characteristic of asthma and may provide useful models to evaluate the efficacy of potential respiratory disease therapies or pharmaceuticals.

Original languageEnglish (US)
Pages (from-to)2143-2156
Number of pages14
JournalJournal of Experimental Medicine
Volume185
Issue number12
DOIs
StatePublished - Jun 16 1997
Externally publishedYes

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Interleukin-5
Eosinophils
Transgenic Mice
Focal Epithelial Hyperplasia
Asthma
Epithelium
Respiratory Therapy
Lung
Genetically Modified Animals
Goblet Cells
Methacholine Chloride
Lymphoid Tissue
Bronchi
Hypertrophy
Collagen
Cytokines
Antigens
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Immunology

Cite this

Lee, J. J., McGarry, M. P., Farmer, S. C., Denzler, K. L., Larson, K. A., Carrigan, P. E., ... Lee, N. A. (1997). Interleukin-5 expression in the lung epithelium of transgenic mice leads to pulmonary changes pathognomonic of asthma. Journal of Experimental Medicine, 185(12), 2143-2156. https://doi.org/10.1084/jem.185.12.2143

Interleukin-5 expression in the lung epithelium of transgenic mice leads to pulmonary changes pathognomonic of asthma. / Lee, James J.; McGarry, Michael P.; Farmer, Steven C.; Denzler, Karen L.; Larson, Kirsten A.; Carrigan, Patricia E.; Brenneise, Ina E.; Horton, Margaret A.; Haczku, Angela Franciska; Gelfand, Erwin W.; Leikauf, George D.; Lee, Nancy A.

In: Journal of Experimental Medicine, Vol. 185, No. 12, 16.06.1997, p. 2143-2156.

Research output: Contribution to journalArticle

Lee, JJ, McGarry, MP, Farmer, SC, Denzler, KL, Larson, KA, Carrigan, PE, Brenneise, IE, Horton, MA, Haczku, AF, Gelfand, EW, Leikauf, GD & Lee, NA 1997, 'Interleukin-5 expression in the lung epithelium of transgenic mice leads to pulmonary changes pathognomonic of asthma', Journal of Experimental Medicine, vol. 185, no. 12, pp. 2143-2156. https://doi.org/10.1084/jem.185.12.2143
Lee, James J. ; McGarry, Michael P. ; Farmer, Steven C. ; Denzler, Karen L. ; Larson, Kirsten A. ; Carrigan, Patricia E. ; Brenneise, Ina E. ; Horton, Margaret A. ; Haczku, Angela Franciska ; Gelfand, Erwin W. ; Leikauf, George D. ; Lee, Nancy A. / Interleukin-5 expression in the lung epithelium of transgenic mice leads to pulmonary changes pathognomonic of asthma. In: Journal of Experimental Medicine. 1997 ; Vol. 185, No. 12. pp. 2143-2156.
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