Interleukin-4 stimulates androgen-independent growth in LNCaP human prostate cancer cells

Ok Lee Soo, Elaine Pinder, Yeon Chun Jae, Wei Lou, Meng Sun, Allen C Gao

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

BACKGROUND. Clinical data showed that the levels of interleukin-4 (IL-4) are significantly elevated in serum of patients with ablation resistant prostate cancer. Previous studies demonstrated that IL-4 enhances androgen receptor (AR) activation mediated by NF-κB in the absence or in the very low levels of androgen in prostate cancer cells. In this study, the role of IL-4 in promoting the growth of androgen-independent prostate cancer cells was examined. METHODS. LNCaP cells were transfected with a full-length IL-4 cDNA and stable clones expressing IL-4 were selected. The growth of LNCaP cells expressing IL-4 was analyzed in vitro and in vivo both in the presence and absence of androgen. RESULTS. Overexpression of IL-4 enhances the growth of androgen-sensitive LNCaP cells in culture media containing charcoal-stripped FBS condition (CS-FBC), and increases the sensitivity of LNCaP cells in response to androgen stimulation. The DHT-mediated cell growth could not be blocked by bicalutamide in IL-4 overexpressing LNCaP cells, but can be neutralized by bicalutamide in parental LNCaP and neo control cells. Furthermore, overexpression of IL-4 stimulates tumor growth of androgen-sensitive LNCaP cells both in intact and castrated male mice. CONCLUSIONS. Overexpression of IL-4 increases the sensitivity of androgen-sensitive LNCaP prostate cancer cells in response to androgen stimulation and enhances the growth of LNCaP cells both in the presence and absence of androgen in vitro and in vivo. These studies suggest that IL-4 plays an important role in promoting androgen-independent prostate cancer growth.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalProstate
Volume68
Issue number1
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Fingerprint

Interleukin-4
Androgens
Prostatic Neoplasms
Growth
Charcoal
Androgen Receptors
Culture Media
Complementary DNA
Clone Cells
Cell Culture Techniques

Keywords

  • AR
  • IL-4
  • Prostate cancer

ASJC Scopus subject areas

  • Urology

Cite this

Interleukin-4 stimulates androgen-independent growth in LNCaP human prostate cancer cells. / Soo, Ok Lee; Pinder, Elaine; Jae, Yeon Chun; Lou, Wei; Sun, Meng; Gao, Allen C.

In: Prostate, Vol. 68, No. 1, 01.01.2008, p. 85-91.

Research output: Contribution to journalArticle

Soo, Ok Lee ; Pinder, Elaine ; Jae, Yeon Chun ; Lou, Wei ; Sun, Meng ; Gao, Allen C. / Interleukin-4 stimulates androgen-independent growth in LNCaP human prostate cancer cells. In: Prostate. 2008 ; Vol. 68, No. 1. pp. 85-91.
@article{e2e62de6e54d407e9f62e39d818be749,
title = "Interleukin-4 stimulates androgen-independent growth in LNCaP human prostate cancer cells",
abstract = "BACKGROUND. Clinical data showed that the levels of interleukin-4 (IL-4) are significantly elevated in serum of patients with ablation resistant prostate cancer. Previous studies demonstrated that IL-4 enhances androgen receptor (AR) activation mediated by NF-κB in the absence or in the very low levels of androgen in prostate cancer cells. In this study, the role of IL-4 in promoting the growth of androgen-independent prostate cancer cells was examined. METHODS. LNCaP cells were transfected with a full-length IL-4 cDNA and stable clones expressing IL-4 were selected. The growth of LNCaP cells expressing IL-4 was analyzed in vitro and in vivo both in the presence and absence of androgen. RESULTS. Overexpression of IL-4 enhances the growth of androgen-sensitive LNCaP cells in culture media containing charcoal-stripped FBS condition (CS-FBC), and increases the sensitivity of LNCaP cells in response to androgen stimulation. The DHT-mediated cell growth could not be blocked by bicalutamide in IL-4 overexpressing LNCaP cells, but can be neutralized by bicalutamide in parental LNCaP and neo control cells. Furthermore, overexpression of IL-4 stimulates tumor growth of androgen-sensitive LNCaP cells both in intact and castrated male mice. CONCLUSIONS. Overexpression of IL-4 increases the sensitivity of androgen-sensitive LNCaP prostate cancer cells in response to androgen stimulation and enhances the growth of LNCaP cells both in the presence and absence of androgen in vitro and in vivo. These studies suggest that IL-4 plays an important role in promoting androgen-independent prostate cancer growth.",
keywords = "AR, IL-4, Prostate cancer",
author = "Soo, {Ok Lee} and Elaine Pinder and Jae, {Yeon Chun} and Wei Lou and Meng Sun and Gao, {Allen C}",
year = "2008",
month = "1",
day = "1",
doi = "10.1002/pros.20691",
language = "English (US)",
volume = "68",
pages = "85--91",
journal = "Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Interleukin-4 stimulates androgen-independent growth in LNCaP human prostate cancer cells

AU - Soo, Ok Lee

AU - Pinder, Elaine

AU - Jae, Yeon Chun

AU - Lou, Wei

AU - Sun, Meng

AU - Gao, Allen C

PY - 2008/1/1

Y1 - 2008/1/1

N2 - BACKGROUND. Clinical data showed that the levels of interleukin-4 (IL-4) are significantly elevated in serum of patients with ablation resistant prostate cancer. Previous studies demonstrated that IL-4 enhances androgen receptor (AR) activation mediated by NF-κB in the absence or in the very low levels of androgen in prostate cancer cells. In this study, the role of IL-4 in promoting the growth of androgen-independent prostate cancer cells was examined. METHODS. LNCaP cells were transfected with a full-length IL-4 cDNA and stable clones expressing IL-4 were selected. The growth of LNCaP cells expressing IL-4 was analyzed in vitro and in vivo both in the presence and absence of androgen. RESULTS. Overexpression of IL-4 enhances the growth of androgen-sensitive LNCaP cells in culture media containing charcoal-stripped FBS condition (CS-FBC), and increases the sensitivity of LNCaP cells in response to androgen stimulation. The DHT-mediated cell growth could not be blocked by bicalutamide in IL-4 overexpressing LNCaP cells, but can be neutralized by bicalutamide in parental LNCaP and neo control cells. Furthermore, overexpression of IL-4 stimulates tumor growth of androgen-sensitive LNCaP cells both in intact and castrated male mice. CONCLUSIONS. Overexpression of IL-4 increases the sensitivity of androgen-sensitive LNCaP prostate cancer cells in response to androgen stimulation and enhances the growth of LNCaP cells both in the presence and absence of androgen in vitro and in vivo. These studies suggest that IL-4 plays an important role in promoting androgen-independent prostate cancer growth.

AB - BACKGROUND. Clinical data showed that the levels of interleukin-4 (IL-4) are significantly elevated in serum of patients with ablation resistant prostate cancer. Previous studies demonstrated that IL-4 enhances androgen receptor (AR) activation mediated by NF-κB in the absence or in the very low levels of androgen in prostate cancer cells. In this study, the role of IL-4 in promoting the growth of androgen-independent prostate cancer cells was examined. METHODS. LNCaP cells were transfected with a full-length IL-4 cDNA and stable clones expressing IL-4 were selected. The growth of LNCaP cells expressing IL-4 was analyzed in vitro and in vivo both in the presence and absence of androgen. RESULTS. Overexpression of IL-4 enhances the growth of androgen-sensitive LNCaP cells in culture media containing charcoal-stripped FBS condition (CS-FBC), and increases the sensitivity of LNCaP cells in response to androgen stimulation. The DHT-mediated cell growth could not be blocked by bicalutamide in IL-4 overexpressing LNCaP cells, but can be neutralized by bicalutamide in parental LNCaP and neo control cells. Furthermore, overexpression of IL-4 stimulates tumor growth of androgen-sensitive LNCaP cells both in intact and castrated male mice. CONCLUSIONS. Overexpression of IL-4 increases the sensitivity of androgen-sensitive LNCaP prostate cancer cells in response to androgen stimulation and enhances the growth of LNCaP cells both in the presence and absence of androgen in vitro and in vivo. These studies suggest that IL-4 plays an important role in promoting androgen-independent prostate cancer growth.

KW - AR

KW - IL-4

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=37449002885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37449002885&partnerID=8YFLogxK

U2 - 10.1002/pros.20691

DO - 10.1002/pros.20691

M3 - Article

C2 - 18008330

AN - SCOPUS:37449002885

VL - 68

SP - 85

EP - 91

JO - Prostate

JF - Prostate

SN - 0270-4137

IS - 1

ER -