Interlaboratory trial for the measurement of total cobalt in equine urine and plasma by ICP-MS

Marie Agnes Popot, Emmie N.M. Ho, Natali Stojiljkovic, Florian Bagilet, Pierre Remy, Pascal Maciejewski, Benoit Loup, George H.M. Chan, Sabine Hargrave, Rick Arthur, Charlie Russo, James White, Pamela Hincks, Clive Pearce, George Ganio, Paul Zahra, David Batty, Mark Jarrett, Lydia Brooks, Lise Anne PrescottLudovic Bailly-Chouriberry, Yves Bonnaire, Terence S.M. Wan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Cobalt is an essential mineral micronutrient and is regularly present in equine nutritional and feed supplements. Therefore, cobalt is naturally present at low concentrations in biological samples. The administration of cobalt chloride is considered to be blood doping and is thus prohibited. To control the misuse of cobalt, it was mandatory to establish an international threshold for cobalt in plasma and/or in urine. To achieve this goal, an international collaboration, consisting of an interlaboratory comparison between 5 laboratories for the urine study and 8 laboratories for the plasma study, has been undertaken. Quantification of cobalt in the biological samples was performed by inductively coupled plasma-mass spectrometry (ICP-MS). Ring tests were based on the analysis of 5 urine samples supplemented at concentrations ranging from 5 up to 500 ng/mL and 5 plasma samples spiked at concentrations ranging from 0.5 up to 25 ng/mL. The results obtained from the different laboratories were collected, compiled, and compared to assess the reproducibility and robustness of cobalt quantification measurements. The statistical approach for the ring test for total cobalt in urine was based on the determination of percentage deviations from the calculated means, while robust statistics based on the calculated median were applied to the ring test for total cobalt in plasma. The inter-laboratory comparisons in urine and in plasma were successful so that 97.6% of the urine samples and 97.5% of the plasma samples gave satisfactory results. Threshold values for cobalt in plasma and urine were established from data only obtained by laboratories involved in the ring test.

Original languageEnglish (US)
Pages (from-to)1400-1406
Number of pages7
JournalDrug Testing and Analysis
Volume9
Issue number9
DOIs
StatePublished - Sep 1 2017

Keywords

  • inductively coupled plasma-mass spectrometry
  • interlaboratory reproducibility
  • plasma
  • total cobalt
  • urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Pharmaceutical Science
  • Spectroscopy

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