Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S. F. Hurst, S. E. Kidd, C. O. Morrissey, E. Snelders, W. J G Melchers, M. V. Castelli, E. Mellado, K. Simmon, C. A. Petti, S. Richardson, S. Zhang, Anna M Romanelli, B. L. Wickes, H. A. De Valk, C. H W Klaassen, S. Arunmozhi Balajee

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

Original languageEnglish (US)
Pages (from-to)1562-1564
Number of pages3
JournalJournal of Clinical Microbiology
Volume47
Issue number5
DOIs
StatePublished - May 2009
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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