Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S. F. Hurst, S. E. Kidd, C. O. Morrissey, E. Snelders, W. J G Melchers, M. V. Castelli, E. Mellado, K. Simmon, C. A. Petti, S. Richardson, S. Zhang, Anna M Romanelli, B. L. Wickes, H. A. De Valk, C. H W Klaassen, S. Arunmozhi Balajee

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

Original languageEnglish (US)
Pages (from-to)1562-1564
Number of pages3
JournalJournal of Clinical Microbiology
Volume47
Issue number5
DOIs
StatePublished - May 2009
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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    Hurst, S. F., Kidd, S. E., Morrissey, C. O., Snelders, E., Melchers, W. J. G., Castelli, M. V., Mellado, E., Simmon, K., Petti, C. A., Richardson, S., Zhang, S., Romanelli, A. M., Wickes, B. L., De Valk, H. A., Klaassen, C. H. W., & Arunmozhi Balajee, S. (2009). Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus. Journal of Clinical Microbiology, 47(5), 1562-1564. https://doi.org/10.1128/JCM.00124-09