Interferons as gene activators: A cluster of six interferon-activatable genes is linked to the erythroid α-spectrin locus on murine chromosome 1

Ghislain Opdenakker, Jay Snoddy, Divaker Choubey, Elena Toniato, Dimitrina D. Pravtcheva, Michael F Seldin, Frank H. Ruddle, Peter Lengyel

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Abstract

Several interferon-activatable murine genes were mapped to murine chromosomes by hybridizing cDNA probes to Southern blots of genomic DNA samples from a panel of mouse-hamster somatic cell hybrid lines. The 12 gene is located on chromosome 12 and it specifies a 3.6-kb mRNA. The 204 gene (specifying a 2.5-kb mRNA), and three genes of the 203 gene family (hybridizing to five mRNAs of sizes between 2 and 4.5 kb), together with the 202 gene (specifying a 2-kb mRNA) are located on murine chromosome 1. By restriction fragment length polymorphism analysis of DNA samples prepared from a panel of recombinant inbred mouse lines (C57BL/6J × DBA/2J) and from 95 [(C3H/HeJ-gld/gld × Mus spretus) F1 × C3H/HeJ-gld/gld] backcross mice we established a close linkage of the 202, 203, and 204 genes to the erythroid α-spectrin gene (Spna-1) on distal murine chromosome 1. Cosmids containing the 202, 203, and 204 genes were isolated from a library derived from AKR mouse DNA. Southern blot analysis of such cosmids revealed: (a) hybridization of a partial 203 cDNA to three genes of the 203 gene family; (b) cross-hybridization of the 202 and 204 genes with one another and with a third gene (designated as 201 gene), and (c) a close linkage of genes of the 203 family with the 201, 202, and 204 genes. These results indicate the existence of a cluster of at least six closely linked, interferon-activatable genes on distal murine chromosome 1 in the vicinity of the Spna-1 locus and also of the Minor lymphocyte stimulating locus (MIsa).

Original languageEnglish (US)
Pages (from-to)568-578
Number of pages11
JournalVirology
Volume171
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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